Methylated, blocked 5' termini of yeast mRNA.

Sripati, Conjeevaram E.; Groner, Yoram; Warner, Jonathan R.

doi:10.1016/s0021-9258(17)33475-0

Cited by 107 publications

(6 citation statements)

References 29 publications

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“…The absence of m6A in duck globin mRNA (Perry and Scherrer, 1975), as well as poly(A)-containing RNA from slime molds (Dottin et al, 1976) and yeast (Sripati et al, 1976) also suggests that the presence of m6A is not required for posttranscriptional cleavage or transport of mRNA. However, since it appears that the poly(A)-containing nuclear RNA from slime molds is only slightly larger than the mRNA from this organism (Firtel and Lodish, 1973), whereas the hn RNA of higher organisms can be several times larger than mRNA (Holmes and Bonner, 1973;Derman and Darnell, 1974), the steps involved in the conversion on nuclear RNA to mRNA may be different in these two cases.…”

Section: Discussionmentioning

confidence: 99%

“…3'-terminal poly(A) sequences (Lai and Duesberg, 1972;Quade et al, 1974) and a capped and methylated S'-terminal structure m7G(5')ppp(5')Gm' (Furuichi et al, 1975c; and Fraenkel-Conrat, 1975; Stoltzfus and Dimock, 1976); both of these structural properties characterize many viral (Furuichi et al, 1975b;Furuichi and Miura, 1975; Lavi and Shatkin, 1975;Moyer et al, 1975;Sommer et al, 1976; and cellular mRNAs (Adams and Cory, 1975;Dottin et al, 1976;Furuichi et al, 1975a;Lavi and Shatkin, 1975;Sripati et al, 1976;Yang et al, 1976). In addition, viral-specific 35S RNA from oncornavirus-infected cells is found associated with polysomes (Fan and Baltimore, 1973) and the genome RNA has been shown to direct the synthesis of viral polypeptides in cell-free translation systems (Siegert et al, 1972;Twardzik et al, 1973; von der Helm and Duesberg, 1975).…”

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confidence: 99%

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Sequence specificity of internal methylation in B77 avian sarcoma virus RNA subunits

Dimock¹,

Stoltzfus²

1977

Biochemistry

117

151

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Following ribonuclease digestion of methyl-3H-labeled B77 avian sarcoma virus RNA subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. Partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. In addition to methylated nucleosides in the 5'-terminal cap structure, m7G(5')GmpCp, N6-methyladenosine(m6A) was found to be present in only two internal sequences of the RNA molecule, Gpm6ApC and Apm6ApC. The average numbers of methylated nucleosides per RNA subunit are about 12-13 in Gpm6ApC, 1-2 in Apm6ApC, and 2 in m7GpppGmpCp. The sequences containing m6A in B77 sarcoma virus RNA are identical to m6A-containing sequences previously reported for the bulk mRNA from HeLa cells (Wei, C.M., Gershowitz, A., and Moss, B. (1976), Biochemistry 15, 397-401). Analysis of the oligonucleotides produced by RNase A digestion indicated that the sequence of bases on the 5' side of these trinucleotides is not specific. The oligonucleotide profile, however, was highly reproducible in different virus preparations. This suggests that the methylations occur at specific positions on the RNA molecule. Some of the methylated oligonucleotides produced by RNase A digestion appear to be present in less than molar amounts. Several hypotheses are proposed to explain this result.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Sequence specificity of internal methylation in B77 avian sarcoma virus RNA subunits

Dimock¹,

Stoltzfus²

1977

Biochemistry

117

151

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“…m 6 A has not previously been observed in the mRNA of the yeast Saccharomyces cerevisiae (16), even though this organism does contain a gene (IME4/SPO8) with striking sequence similarity to the mammalian gene encoding the N 6 -adenosine methyltransferase subunit, MT-A70 (17). This sequence similarity raised the possibility that yeast mRNA does contain m 6 A, but only under nutritional conditions conducive to high expression of the IME4 gene.…”

Section: Introductionmentioning

confidence: 96%

Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene

Clancy¹

2002

Nucleic Acids Research

271

286

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N6-methyladenosine (m6A) is present at internal sites in mRNA isolated from all higher eukaryotes, but has not previously been detected in the mRNA of the yeast Saccharomyces cerevisiae. This nucleoside modification occurs only in a sequence- specific context that appears to be conserved across diverse species. The function of this modification is not fully established, but there is some indirect evidence that m6A may play a role in the efficiency of mRNA splicing, transport or translation. The S.cerevisiae gene IME4, which is important for induction of sporulation, is very similar to the human gene MT-A70, which has been shown to be a critical subunit of the human mRNA [N6-adenosine]-methyltransferase. This observation led to the hypothesis that yeast sporulation may be dependent upon methylation of yeast mRNA, mediated by Ime4p. In this study we show that induction of sporulation leads to the appearance of low levels of m6A in yeast mRNA and that this modification requires IME4. Moreover, single amino acid substitutions in the putative catalytic residues of Ime4p lead to severe sporulation defects in a strain whose sporulation ability is completely dependent on this protein. Collectively, these data suggest very strongly that the activation of sporulation by Ime4p is the result of its proposed methyltransferase activity and provide the most direct evidence to date of a physiologic role of m6A in a gene regulatory pathway.

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“…In higher eukaryotes, the 2′O of the ribose of the first base or first and second base is methylated, creating the predominant cap 1 and cap 2 structures, respectively (Furuichi et al ., 1975; Bélanger et al ., 2010; Werner et al ., 2011; Furuichi, 2015); the responsible human methylases are hMtr1 and hMtr2 (Bélanger et al ., 2010; Werner et al ., 2011). Ribose methylations are absent in yeast (Mager et al ., 1976; Sripati et al ., 1976). In metazoans, the 5′ m 7 G cap is important for the export of spliced mRNAs but not of intron-less mRNAs, probably because it is involved in recruiting the TREX complex upstream to the first exon−exon junction (Cheng et al ., 2006).…”

Section: Nuclear Mrna Processing Steps In Opisthokonts and Trypanosomesmentioning

confidence: 99%

Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts

Krämer

2021

Parasitology

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Methylated, blocked 5' termini of yeast mRNA.

Cited by 107 publications

References 29 publications

Sequence specificity of internal methylation in B77 avian sarcoma virus RNA subunits

Sequence specificity of internal methylation in B77 avian sarcoma virus RNA subunits

Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene

Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts

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