Methyl Vitamin B12 but not methylfolate rescues a motor neuron-like cell line from homocysteine-mediated cell death

Hemendinger, Richelle; Armstrong, Edward J.; Brooks, Benjamin Rix

doi:10.1016/j.taap.2011.01.003

Cited by 23 publications

(16 citation statements)

References 85 publications

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“…In the cells, vit B 12 is transferred to the cytoplasm by the lysosomal membrane vit B 12 transporter (LMBRD1) and serves as cofactor for methyl malonylCoA mutase (Mut) and methionine synthase (MS) [21]. Futhermore, vit B 12 deficiency leads to methionine deficiency, which is required for the synthesis of both phospholipids and myelin.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Neuropathological and neuroprotective features of vitamin B12 on the dorsal spinal ganglion of rats after the experimental crush of sciatic nerve: an experimental study

et al. 2013

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BackgroundSpinal motoneuron neuroprotection by vitaminB12 was previously reported; the present study was carried out to evaluate neuroprotectivity in the dorsal root ganglion sensory neuron.MethodsIn present study thirty-six Wister-Albino rats (aged 8–9 weeks and weighing 200–250 g) were tested. The animals were randomly divided into 6 groups which every group contained 6 rats. Group A: received normal saline (for 42 days); Group B: vitamin B12 was administered (0.5 mg/kg/day for 21 days); Group C: received vitamin B12 (1 mg/kg/day for 21days); Group D: received vitamin B12 (0.5 mg/kg/day for 42 days); Group E; received vitamin B12 (1 mg/kg/day for 42 days); Group F; received no treatment. The L5 Dorsal Root Ganglion (DRG) neurons count compared to the number of left and right neurons .Furthermore, DRG sensory neurons for regeneration were evaluated 21 or 42 days after injury (each group was analyzed by One-Way ANOVA test).Results(1): The comparison of left crushed neurons (LCN) number with right non-crushed neurons in all experimental groups (B, C, D and C), indicating a significant decline in their neurons enumeration (p<0/05). (2): The comparison of test group’s LCN with the control group’s LCN revealed a significant rise in the number of experimental group neurons (p<0/05). (3): Moreover, comparing the number of right neurons in experimental groups with the number of neurons in crushed neurons indicated that the average number of right neurons showed a significant increase in experimental groups (p<0/05).ConclusionConsequently, the probability of nerve regeneration will be increased by the increment of the administered drug dosage and duration. On the other hand, the regeneration and healing in Dorsal Spinal Ganglion will be improved by increase of administration time and vitamin B12 dose, indicating that such vitamin was able to progress recovery process of peripheral nerves damage in experimental rats. Finally, our results have important implications for elucidating the mechanisms of nerve regeneration. Moreover, the results showed that vitaminB12 had a proliferative effect on the dorsal root ganglion sensory neuron.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7395141841009256

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Neuropathological and neuroprotective features of vitamin B12 on the dorsal spinal ganglion of rats after the experimental crush of sciatic nerve: an experimental study

et al. 2013

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“…High-dose methylcobalamin was also reported to protect cortical neurons against glutamate toxicity [24]. Elevated homocysteine levels have been suggested to be toxic for motor neurons [25], and methylcobalamin protected motor neurons from homocysteine-induced toxicity [26]. These profiles are relevant to ALS patients because plasma homocysteine levels were significantly increased in patients who have shorter time to diagnosis of ALS [27].…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effect of ultra-high dose methylcobalamin in wobbler mouse model of amyotrophic lateral sclerosis

Ikeda

Iwasaki

Kaji

2015

Journal of the Neurological Sciences

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“…However, in one study, B12 alone did not show any effect on homocysteine levels, onset of disease or survival. However, a more recent in vitro study had opposite results, showing better survival against homocysteine toxicity in vitro, in cells pretreated with B12 but not in those treated with folate (75). Furthermore, in one small pre-clinical study, ALS mice were supplemented with Galactooligosaccharides (GOS), a prebiotic that is thought to improve the absorption and synthesis of B Vitamins.…”

Section: Types Of Cam That Pals Commonly Ask Aboutmentioning

confidence: 93%

Complementary and Alternative Therapies in Amyotrophic Lateral Sclerosis

Bedlack

Joyce

Carter³

et al. 2015

Neurologic Clinics

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Synopsis Given the severity of their illness and lack of effective disease modifying agents, it is not surprising that most patients with ALS consider trying complementary and alternative therapies. Some of the most commonly considered alternative therapies include special diets, nutritional supplements, cannabis, acupuncture, chelation and energy healing. This chapter reviews these in detail. We also describe 3 models by which physicians may frame discussions about alternative therapies: paternalism, autonomy and shared decision making. Finally, we review a program called ALSUntangled which using shared shared decision making to review alternative therapies for ALS.

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Methyl Vitamin B12 but not methylfolate rescues a motor neuron-like cell line from homocysteine-mediated cell death

Cited by 23 publications

References 85 publications

RETRACTED ARTICLE: Neuropathological and neuroprotective features of vitamin B12 on the dorsal spinal ganglion of rats after the experimental crush of sciatic nerve: an experimental study

RETRACTED ARTICLE: Neuropathological and neuroprotective features of vitamin B12 on the dorsal spinal ganglion of rats after the experimental crush of sciatic nerve: an experimental study

Neuroprotective effect of ultra-high dose methylcobalamin in wobbler mouse model of amyotrophic lateral sclerosis

Complementary and Alternative Therapies in Amyotrophic Lateral Sclerosis

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