Methyl Salicylate Level Increase in Flax after Fusarium oxysporum Infection Is Associated with Phenylpropanoid Pathway Activation

Boba, Aleksandra; Kostyń, Kamil; Kostyn, Anna; Wojtasik, Wioleta; Dziadas, Mariusz; Preisner, Marta; Szopa, Jan; Kulma, Anna

doi:10.3389/fpls.2016.01951

Cited by 30 publications

(35 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This overlap included the cytokinin oxidase Ckx3 (Lj5g3v0692300) that is involved in cytokinin breakdown during nodulation ( Reid et al, 2016 ) and the sulfate transporter Sst1 (Lj2g3v0776860.1) that is required for nitrogen-fixation within nodules. In addition, putative defense-related genes identified were a PR10-like gene (Lj0g3v0286359), the WAT1-related gene (Lj1g3v0913340) and a gene encoding a salicylate O -methyltransferase-like protein (Lj6g3v0509430) that is involved in the biosynthesis of methyl salicylate which was found to participate in root responses against fungal pathogens ( Ament et al, 2010 ; Boba et al, 2016 ). Further studies will be required to determine the roles of the symbiosis-related genes in the Lotus – Ralstonia interaction and of the putative defense genes in Lotus –rhizobium interactions.…”

Section: Resultsmentioning

confidence: 99%

Distinct Lotus japonicus Transcriptomic Responses to a Spectrum of Bacteria Ranging From Symbiotic to Pathogenic

et al. 2018

View full text Add to dashboard Cite

Lotus japonicus is a well-studied nodulating legume and a model organism for the investigation of plant-microbe interactions. The majority of legume transcriptome studies have focused on interactions with compatible symbionts, whereas responses to non-adapted rhizobia and pathogenic bacteria have not been well-characterized. In this study, we first characterized the transcriptomic response of L. japonicus to its compatible symbiont, Mesorhizobium loti R7A, through RNA-seq analysis of various plant tissues. Early symbiotic signaling was largely Nod factor-dependent and enhanced within root hairs, and we observed large-scale transcriptional reprogramming in nodule primordia and mature nitrogen-fixing nodules. We then characterized root transcriptional responses to a spectrum of L. japonicus interacting bacteria ranging from semi-compatible symbionts to pathogens. M. loti R7A and the semi-compatible strain Sinorhizobium fredii HH103 showed remarkably similar responses, allowing us to identify a small number of genes potentially involved in differentiating between fully and semi-compatible symbionts. The incompatible symbiont Bradyrhizobium elkanii USDA61 induced a more attenuated response, but the weakest response was observed for the foliar pathogen Pseudomonas syringae pv. tomato DC3000, where the affected genes also responded to other tested bacteria, pointing to a small set of common bacterial response genes. In contrast, the root pathogen Ralstonia solanacearum JS763 induced a pronounced and distinct transcriptomic pathogen response, which we compared to the results of the other treatments. This comparative analysis did not support the concept that an early defense-like response is generally evoked by compatible rhizobia during establishment of symbiosis.

show abstract

Section: Resultsmentioning

confidence: 99%

Distinct Lotus japonicus Transcriptomic Responses to a Spectrum of Bacteria Ranging From Symbiotic to Pathogenic

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The plant employs a number of secondary metabolites in the defense against the pathogen [28]. It has already been verified that phenylpropanoids are part of the chemical defense system of plants against parasitic nematodes [29].…”

Section: Discussionmentioning

confidence: 99%

Soybean root transcriptome profiling reveals a nonhost resistant response during Heterodera glycines infection

Song

Liang

et al. 2019

PLoS ONE

View full text Add to dashboard Cite

Heterodera glycines (soybean cyst nematode, SCN) is one of the most devastating pathogens of soybean worldwide. The compatible and in compatible interactions between soybean and SCN have well documented. Nevertheless, the molecular mechanism of a nonhost resistant response in soybean against SCN infection remains obscure. Toward this end, a global transcriptional comparison was conducted between susceptible and resistant reactions of soybean roots infected by taking advantage of finding a new pathotype of SCN (SCN T ). The soybean cultivar Lee, which exhibits resistant to SCN T and susceptible to HG 1.2.3.4.7 (SCN s ) was utilized in the expriments. The results highlighted a nonhost resistant response of soybean. Transcriptome analysis indicated that the number of differentially expressed genes (DEGs) in the resistant interaction (3746) was much larger than that in the susceptible interaction (602). A great number of genes acting as intrinsic component of membrane, integral component of membrane, cell periphery and plasma membrance were remarkably enriched only in the resistant interaction, while the taurine and hypotaurine, phenylpropanoid pathway, plant-pathogen interaction and transcript factors were modulated in both interactions. This is the first study to examine genes expression patterns in a soybean genotype in response to invasion by a virulent and avirulent SCN population at the transcriptional level, which will provide insights into the complicate molecular mechanism of the nonhost resistant interaction.

show abstract

“…These secondary metabolites with antioxidative properties can be divided into two groups. Those produced via the phenylpropanoid pathway (phenolic acids, flavonoids, lignin and lignans) have been proven to participate in plant defense against pathogen invasion (Kostyn et al 2012;Boba et al 2016). The second group consists of isoprenoids, also called terpenoids.…”

Section: Introductionmentioning

confidence: 99%

Fusarium oxysporum infection activates the plastidial branch of the terpenoid biosynthesis pathway in flax, leading to increased ABA synthesis

Boba

Kostyń

Kozak

et al. 2020

Planta

Self Cite

View full text Add to dashboard Cite

Main conclusion Upregulation of the terpenoid pathway and increased ABA content in flax upon Fusarium infection leads to activation of the early plant's response (PR genes, cell wall remodeling, and redox status).Abstract Plants have developed a number of defense strategies against the adverse effects of fungi such as Fusarium oxysporum. One such defense is the production of antioxidant secondary metabolites, which fall into two main groups: the phenylpropanoids and the terpenoids. While functions and biosynthesis of phenylpropanoids have been extensively studied, very little is known about the genes controlling the terpenoid synthesis pathway in flax. They can serve as antioxidants, but are also substrates for a plethora of different compounds, including those of regulatory functions, like ABA. ABA's function during pathogen attack remains obscure and often depends on the specific plant-pathogen interactions. In our study we showed that in flax the non-mevalonate pathway is strongly activated in the early hours of pathogen infection and that there is a redirection of metabolites towards ABA synthesis. The elevated synthesis of ABA correlates with flax resistance to F. oxysporum, thus we suggest ABA to be a positive regulator of the plant's early response to the infection.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

show abstract

Methyl Salicylate Level Increase in Flax after Fusarium oxysporum Infection Is Associated with Phenylpropanoid Pathway Activation

Cited by 30 publications

References 59 publications

Distinct Lotus japonicus Transcriptomic Responses to a Spectrum of Bacteria Ranging From Symbiotic to Pathogenic

Distinct Lotus japonicus Transcriptomic Responses to a Spectrum of Bacteria Ranging From Symbiotic to Pathogenic

Soybean root transcriptome profiling reveals a nonhost resistant response during Heterodera glycines infection

Fusarium oxysporum infection activates the plastidial branch of the terpenoid biosynthesis pathway in flax, leading to increased ABA synthesis

Contact Info

Product

Resources

About