Methylcobalamin, the active form of vitamin B 12 , is a vital supplement for vitamin B 12 deficiency. Hydrotropic antisolvent crystallization was utilized for reducing methylcobalamin particle size as it offers advantages like enhancing the processing capacity, using solvents with low toxicity and low risk to human health. Recently, environmental awareness has fostered the use of life cycle assessment (LCA) for evaluating the environmental impacts of various processes. In the present study, a comparative study of the LCA approach has been adopted for producing methylcobalamin nanoparticles using hydrotropic antisolvent crystallization and antisolvent crystallization. In both processes, production of 1 mg of methylcobalamin nanoparticles was selected as a functional unit to conduct the impact assessment. SimaPro software was used to carry out the LCA study. The analysis has indicated that the centrifuging and washing, and drying stages have significant impact on the environmental categories under consideration. The incorporation of a hydrotrope in the antisolvent crystallization process has reduced the processing cost and environmental impact. The hydrotropic antisolvent crystallization is more sustainable as it lowers the environmental impact by ∼35% compared to antisolvent crystallization.