Methyl jasmonate treatment regulates α-linolenic acid metabolism and jasmonate acid signaling pathway to improve chilling tolerance in both stony hard and melting flesh peaches

Chen, Huan; Yang, Xiaohan; Wang, Lufan; Kebbeh, Mariama; Wang, Yuxuan; Dai, Binger; Shen, Shuling; Zheng, Xiaolin; Zhou, Huijuan

doi:10.1016/j.postharvbio.2022.111960

Cited by 32 publications

(7 citation statements)

References 44 publications

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“…This protects the integrity of SlLOX5 and SlAOS2 and promotes the JA biosynthesis. Studies have suggested that MeJA can improve putrescine biosynthesis to enhance tomato cold tolerance, while inhibition of MeJA biosynthesis significantly reduces the cold tolerance of tomato plants. , In addition, MeJA treatment activated the activities of PpLOX, PpAOS, and PpAOC under cold stress . In this study, the SlCPase -silenced plants showed stronger tolerance than the control plants under low temperatures (Figure A).…”

Section: Discussionsupporting

confidence: 53%

“…51,52 In addition, MeJA treatment activated the activities of PpLOX, PpAOS, and PpAOC under cold stress. 53 In this study, the SlCPase-silenced plants showed stronger tolerance than the control plants under low temperatures (Figure 6A). Moreover, exogenous galactinol has been demonstrated to improve tomato cold tolerance.…”

Section: Angols1 Overexpression Affects Gene Transcription Levels In ...supporting

confidence: 48%

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Galactinol Regulates JA Biosynthesis to Enhance Tomato Cold Tolerance

Liu,

Zhang,

Meng

et al. 2024

J. Agric. Food Chem.

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Low temperatures can inhibit plant growth and development and reduce fruit yield. This study demonstrated that the expression of AnGolS1 from Ammopiptanthus nanus (A. nanus) encoding a galactinol synthase enhanced tomato cold tolerance. In AnGolS1-overexpressing plants, the jasmonic acid (JA) biosynthesis substrates 13-hydroperoxylinolenicacid and 12,13epoxylinolenicacid were significantly accumulated, and the expression levels of the ethylene response factor (SlERF4-7) and serine protease inhibitor (SlSPI5) were increased. We speculated that there may be correlations among galactinol, ethylene signaling, the protease inhibitor, protease, and JA levels. The expression levels of SlERF4-7 and SlSPI5 as well as the JA content were significantly increased under exogenous galactinol treatment. Additionally, the expression of SlSPI5 was reduced in SlERF4-7silenced plants, and SlERF4-7 was confirmed to bind to the dehydration-responsive element (DRE) of the SlSPI5 promoter. These results suggest that SlSPI5 is a target gene of the SlERF4-7 transcription factor. In addition, SlSPI5 interacted with cysteine protease (SlCPase), while SlCPase interacted with lipoxygenase (SlLOX5) and allene oxide synthase (SlAOS2). When SlCPase was silenced, JA levels increased and plant cold tolerance was enhanced. Therefore, galactinol regulates JA biosynthesis to enhance tomato cold tolerance through the SlERF4-7-SlSPI5-SlCPase-SlLOX5/SlAOS2 model. Overall, our study provides new perspectives on the role of galactinol in the JA regulatory network in plant adaptation to low-temperature stress.

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Section: Discussionsupporting

confidence: 53%

Section: Angols1 Overexpression Affects Gene Transcription Levels In ...supporting

confidence: 48%

Galactinol Regulates JA Biosynthesis to Enhance Tomato Cold Tolerance

Liu,

Zhang,

Meng

et al. 2024

J. Agric. Food Chem.

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“…Moreover, ALA is a precursor for n-3 polyunsaturated FAs (PUFA), including DHA, usually called ‘a fatty acid of adaptation’ [ 52 , 58 ]. Considering that exogenous MJ increases the activity of lipoxygenase, the key enzyme of FA synthesis [ 59 ], and the content of endogenous MJ [ 19 ], it can be supposed that MJ is capable of self-biosynthesis regulation by promoting MJ synthesis de novo or its demethylation. However, this assumption needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

Does Methyl Jasmonate Effectively Protect Plants under Heavy Metal Contamination? Fatty Acid Content in Wheat Leaves Exposed to Cadmium with or without Exogenous Methyl Jasmonate Application

et al. 2023

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The effect of methyl jasmonate (MJ) (1 µM) on wheat (Triticum aestivum L. cv. Moskovskaya 39), seedlings and the fatty acid (FA) content of leaves under optimal and cadmium (Cd) (100 µM) stress conditions wasinvestigated. Height and biomass accumulation was studied traditionally; the netphotosynthesis rate (Pn) was studied using a photosynthesis system, FAs’profile—GS-MS. No effect on the height and Pn rate of the MJ pre-treatment wheat at optimum growth conditions was found. MJ pre-treatment led to a decrease in the total amount of saturated (about 11%) and unsaturated (about 17%) identified FAs, except α-linoleic FA (ALA), which is probably associated with its involvement in energy-dependent processes. Under Cd impact, the MJ-treated plants had a higher biomass accumulation and Pn rate compared to untreated seedlings. Both MJ and Cd caused stress-induced elevation of palmitic acid (PA) versus an absence of myristic acid (MA), which is used for elongation. It is suggested that PA participates in alternative adaptation mechanisms (not only as a constituent of the lipid bilayer of biomembrane) of plants under stress. Overall, the dynamics of FAs showed an increase in the saturated FA that is important in the packing of the biomembrane. It is supposed that the positive effect of MJ is associated with lower Cd content in plants and a higher ALA content in leaves.

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“…KEGG enrichment and WGCNA analysis of MeJA-treated P. grandiflorus root transcriptomic data ( Figs. 2A–C, 3A–C ) identified the alpha-linolenic acid metabolism pathway, which was involved in the MeJA biosynthesis pathway [ 36 ], the glycolysis/gluconeogenesis, and starch and sucrose metabolism, which provide the key substrates pyruvic acid, glyceraldehyde 3-phosphate, and acetyl-coenzyme A for the MVA and MEP pathways [ 1 ]. Significant enrichment was also observed in the triterpene saponin backbone biosynthesis and the ABC transporters pathways, which were involved in saponin transportation and biosynthesis [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

RNA sequencing analysis reveals PgbHLH28 as the key regulator in response to methyl jasmonate-induced saponin accumulation in Platycodon grandiflorus

Zhang,

Fan

et al. 2024

Horticulture Research

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Platycodon grandiflorus (Jacq.) A. DC, known for its saponin content, can potentially prevent and treat cerebrovascular diseases and COVID-19. Triterpenoid saponin biosynthesis in plants is enhanced by methyl jasmonate (MeJA) application. However, the underlying molecular mechanisms of MeJA-induced saponin biosynthesis remain unknown in P. grandiflorus. In the current study, exogenous application of 100 μmol/L MeJA was identified to be optimal for promoting saponin accumulation. RNA sequencing analysis demonstrated the PgbHLH28 gene as a key regulatory factor responding to MeJA during saponin accumulation. Overexpression of PgbHLH28 in P. grandiflorus increased saponin content, while silencing of PgbHLH28 significantly inhibited saponin synthesis, suggesting that PgbHLH28 acts as a positive regulator of saponin biosynthesis. Yeast one-hybrid and dual luciferase assays demonstrated that PgbHLH28 directly bound to the promoters of PgHMGR2 and PgDXS2 to activate gene expression. PgHMGR2 and PgDXS2 transformation promoted saponin accumulation, while silencing of these genes inhibited saponin biosynthesis. This study determined that MeJA promoted saponin accumulation in P. grandiflorus by inducing PgbHLH28 gene expression and activating downstream genes (PgHMGR2 and PgDXS2) involved in saponin biosynthesis. In conclusion, a complex regulatory network governing saponin biosynthesis following MeJA treatment was elucidated, offering a theoretical foundation for enhancing saponin content and biosynthesis efficacy in P. grandiflorus.

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Methyl jasmonate treatment regulates α-linolenic acid metabolism and jasmonate acid signaling pathway to improve chilling tolerance in both stony hard and melting flesh peaches

Cited by 32 publications

References 44 publications

Galactinol Regulates JA Biosynthesis to Enhance Tomato Cold Tolerance

Galactinol Regulates JA Biosynthesis to Enhance Tomato Cold Tolerance

Does Methyl Jasmonate Effectively Protect Plants under Heavy Metal Contamination? Fatty Acid Content in Wheat Leaves Exposed to Cadmium with or without Exogenous Methyl Jasmonate Application

RNA sequencing analysis reveals PgbHLH28 as the key regulator in response to methyl jasmonate-induced saponin accumulation in Platycodon grandiflorus

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