Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions

Abstract

Vitamin C (L-ascorbic acid) is an important primary metabolite of plants that functions as an antioxidant, an enzyme cofactor, and a cell-signalling modulator in a wide array of crucial physiological processes, including biosynthesis of the cell wall, secondary metabolites and phytohormones, stress resistance, photoprotection, cell division, and growth. Plants synthesize ascorbic acid via de novo and salvage pathways, but the regulation of its biosynthesis and the mechanisms behind ascorbate homeostasis are largely unknown. Jasmonic acid and its methyl ester (jasmonates) mediate plant responses to many biotic and abiotic stresses by triggering a transcriptional reprogramming that allows cells to cope with pathogens and stress. By using 14C-mannose radiolabelling combined with HPLC and transcript profiling analysis, it is shown that methyl jasmonate treatment increases the de novo synthesis of ascorbic acid in Arabidopsis and tobacco Bright Yellow-2 (BY-2) suspension cells. In BY-2 cells, this stimulation coincides with enhanced transcription of at least two late methyl jasmonate-responsive genes encoding enzymes for vitamin C biosynthesis: the GDP-mannose 3'',5''-epimerase and a putative L-gulono-1,4-lactone dehydrogenase/oxidase. As far as is known, this is the first report of a hormonal regulation of vitamin C biosynthesis in plants. Finally, the role of ascorbic acid in jasmonate-regulated stress responses is reviewed.

Branch girdling at fruit green mature stage affects fruit ascorbic acid contents and expression of genes involved inl-galactose pathway in citrus

“…The response of carbohydrate-related gene expression to girdling might be subjected to the regulation of sugar as a signal (Li et al 2003a, b). Some reports in other crops pointed out that the expression of AA-related genes was stimulated by feeding sucrose or methyl jasmonate (Nishikawa et al 2005;Wolucka et al 2005). In this study, we found that the sucrose content was significantly increased at 20 DAT (Fig.…”

TIME FOR COFFEE Represses Accumulation of the MYC2 Transcription Factor to Provide Time-of-Day Regulation of Jasmonate Signaling in Arabidopsis

“…Such a mechanism may allow plants to promote growth in the evening with less-active JA signaling and restrict growth in the morning when JA response is more active. Oxidative stress caused by the dark-tolight transition is one example of a daily event in which JA signaling is vital to plants (Sasaki-Sekimoto et al, 2005;Wolucka et al, 2005;Dombrecht et al, 2007). In previous genomic analysis, clock-regulated genes responsive to the reactive oxygen species or to oxidative damage were shown to be expressed early in the morning phase (Covington et al, 2008).…”

Response of Salt-Stressed Wheat ( Triticum aestivum L.) to Potassium Humate Treatment and Potassium Silicate Foliar Application

“…Damaging of membranes fatty acids could produce small hydrocarbon fragments including MDA which is an important sign of membrane system injury (Masood et al, 2006 andMoussa, 2008). Ascorbic acid is an important water-soluble antioxidant and cell moderator in physiological processes including photoprotection (Wolucka, 2005 andKhan &Ashraf, 2008) where, it can rapidly react with radical intermediates of an autoxidation chain and stop it (Khan, 2006). Latef (2014) reported that salt stress induced oxidative stress by increasing malondialdehyde (MDA) content and activity of peroxidase.…”