Methyl jasmonate mediates melatonin-induced cold tolerance of grafted watermelon plants

Li, Hao; Guo, Yanliang; Lan, Zhixiang; Xu, Kai; Chang, Jianmei; Ahammed, Golam Jalal; Ma, Junsheng; Wei, Chunhua; Zhang, Xian

doi:10.1038/s41438-021-00496-0

Cited by 100 publications

(81 citation statements)

References 49 publications

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“…S2). This supported by a previous study that showed the initial H 2 O 2 burst is followed by a burst of melatonin, which further triggers downstream signaling to respond to diverse stress (Gong et al 2017;Li et al 2021). Chemical scavenging of H 2 O 2 partially blocked the induction of seed germination and root growth induced by melatonin or BRs following exposure to cold and drought stress (Figs.…”

Section: Brassinosteroid Biosynthesis and Its Signaling Pathway Are Induced By Melatoninsupporting

confidence: 84%

Melatonin-induced cold and drought tolerance requires brassinosteroids and hydrogen peroxide signaling in perennial ryegrass

Fu¹,

Zhang²,

Jiang³

et al. 2021

Preprint

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Aims The interplay between melatonin and brassinosteroids in enhancing tolerance to cold and drought and the underlying molecular mechanisms of this relationship still remain unknown. Methods Combined physiological and transcriptomic analyses were used to clarify the crosstalk of melatonin and brassinosteroids in perennial ryegrass. Brassinosteroids biosynthesis inhibitor propiconazole, Arabidopsis brassinosteroids-receptor mutant bri1-9, bak1, and H2O2 deficient rbohC and rbohF mutants were used to analyzed the role of H2O2 in melatonin and brassinosteroids-mediated tolerance to the stress. Results Melatonin-enhanced cold and drought tolerance of perennial ryegrass depends on the duration of dose and stress that were applied. Melatonin activates the expression of NADPH oxidase-related genes to promote H2O2 production when exposed to short-term cold/drought stresses but reduced accumulation of H2O2 under long-term stress. This was associated with an increase in enzymatic and non-enzymatic antioxidant activities by regulation of their gene expression and modulation of the ascorbate-glutathione cycle. Moreover, exogenous melatonin increased the biosynthesis of melatonin and brassinosteroids or the expression of signaling-related genes. However, the effects of melatonin on the expression of BR biosynthesis and signaling-related genes were inhibited in the rbohC and rbohF mutants. Chemical scavenging of H2O2 attenuated melatonin-mediated growth in the Arabidopsis wild-type, but the bak1 and bri1.9 mutants were relatively insensitive. Consistently, Inhibition of H2O2 production impaired the effect of melatonin and brassinosteroids on seed germination and the root growth of perennial ryegrass. Conclusions This research reveals a novel regulatory mechanism of the crosstalk of H2O2 and brassinosteroid signaling in melatonin-induced cold/drought tolerance in perennial ryegrass.

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Section: Brassinosteroid Biosynthesis and Its Signaling Pathway Are Induced By Melatoninsupporting

confidence: 84%

Melatonin-induced cold and drought tolerance requires brassinosteroids and hydrogen peroxide signaling in perennial ryegrass

Fu¹,

Zhang²,

Jiang³

et al. 2021

Preprint

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“…Therefore, flavonol biosynthesis and BR signaling have important functions in plant resistance, and during this process, ROS act as plant signals to respond to environmental changes 48 . In watermelon, the H 2 O 2 content increased and improved adaptation to 4 °C treatment 49 . In our study, the flavonol levels in the McMYB4 -OE lines grown at 28 °C and 18 °C were increased by 493 μg/g and 2715 μg/g, respectively, compared with those in the control treatment, and the BR contents were increased by 14 μg/L and 18 μg/L in these lines, respectively.…”

Section: Discussionmentioning

confidence: 97%

McMYB4 improves temperature adaptation by regulating phenylpropanoid metabolism and hormone signaling in apple

Hao

Peng

et al. 2021

Hortic Res

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Temperature changes affect apple development and production. Phenylpropanoid metabolism and hormone signaling play a crucial role in regulating apple growth and development in response to temperature changes. Here, we found that McMYB4 is induced by treatment at 28 °C and 18 °C, and McMYB4 overexpression results in flavonol and lignin accumulation in apple leaves. Yeast one-hybrid (Y1H) assays and electrophoretic mobility shift assays (EMSAs) further revealed that McMYB4 targets the promoters of the flavonol biosynthesis genes CHS and FLS and the lignin biosynthesis genes CAD and F5H. McMYB4 expression resulted in higher levels of flavonol and lignin biosynthesis in apple during growth at 28 °C and 18 °C than during growth at 23 °C. At 28 °C and 18 °C, McMYB4 also binds to the AUX/ARF and BRI/BIN promoters to activate gene expression, resulting in acceleration of the auxin and brassinolide signaling pathways. Taken together, our results demonstrate that McMYB4 promotes flavonol biosynthesis and brassinolide signaling, which decreases ROS contents to improve plant resistance and promotes lignin biosynthesis and auxin signaling to regulate plant growth. This study suggests that McMYB4 participates in the abiotic resistance and growth of apple in response to temperature changes by regulating phenylpropanoid metabolism and hormone signaling.

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“…Many studies have shown that H 2 O 2 plays an essential signaling role in melatonin-mediated regulation of various physiological processes, including stomatal closure, seed germination, lateral root formation, and response to environmental stimulus [ 19 , 29 , 60 , 61 ]. A recent report revealed that H 2 O 2 mediates melatonin-induced cold tolerance in grafted watermelon plants [ 24 ]. In this study, melatonin induced H 2 O 2 accumulation and ClRBOHD expression in early response (within 6 h) to chilling stress in watermelon ( Figure 1 A).…”

Section: Discussionmentioning

confidence: 99%

“…To evaluate the influences of melatonin, H 2 O 2 , or Ca 2+ on watermelon response to cold stress, the seedlings were sprayed with 150 µM melatonin [ 24 ], H 2 O 2 (0.04, 0.2, 1, 5 mM), 20 mM CaCl 2 [ 30 , 31 ], or distilled water (as the control). Melatonin (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in ethanol and then diluted with distilled water at a ratio of 1:10,000 [ethanol:water; v : v ].…”

Section: Methodsmentioning

confidence: 99%

“…Exogenous melatonin has been shown to strengthen chilling tolerance in diverse plant species by scavenging excessive ROS and improving the photosynthesis system [ 21 , 22 , 23 ]. A recent study has shown that melatonin, as a potential root-to-shoot signal, induces the increases in methy jasmonate and H 2 O 2 in rootstock-scion communication in response to cold stress [ 24 ]. Melatonin induces the expression of CBFs and COR genes under cold stress, suggesting that the CBF pathway may participate in melatonin-induced cold tolerance [ 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Positive Interaction between H2O2 and Ca2+ Mediates Melatonin-Induced CBF Pathway and Cold Tolerance in Watermelon (Citrullus lanatus L.)

Chang

Guo

et al. 2021

Antioxidants

Self Cite

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Cold stress is a major environmental factor that detrimentally affects plant growth and development. Melatonin has been shown to confer plant tolerance to cold stress through activating the C-REPEAT BINDING FACTOR (CBF) pathway; however, the underlying modes that enable this function remain obscure. In this study, we investigated the role of H2O2 and Ca2+ signaling in the melatonin-induced CBF pathway and cold tolerance in watermelon (Citrullus lanatus L.) through pharmacological, physiological, and genetic approaches. According to the results, melatonin induced H2O2 accumulation, which was associated with the upregulation of respiratory burst oxidase homolog D (ClRBOHD) during the early response to cold stress in watermelon. Besides, melatonin and H2O2 induced the accumulation of cytoplasmic free Ca2+ ([Ca2+]cyt) in response to cold. This was associated with the upregulation of cyclic nucleotide-gated ion channel 2 (ClCNGC2) in watermelon. However, blocking of Ca2+ influx channels abolished melatonin- or H2O2-induced CBF pathway and cold tolerance. Ca2+ also induced ClRBOHD expression and H2O2 accumulation in early response to cold stress in watermelon. Inhibition of H2O2 production in watermelon by RBOH inhibitor or in Arabidopsis by AtRBOHD knockout compromised melatonin-induced [Ca2+]cyt accumulation and melatonin- or Ca2+-induced CBF pathway and cold tolerance. Overall, these findings indicate that melatonin induces RBOHD-dependent H2O2 generation in early response to cold stress. Increased H2O2 promotes [Ca2+]cyt accumulation, which in turn induces H2O2 accumulation via RBOHD, forming a reciprocal positive-regulatory loop that mediates melatonin-induced CBF pathway and subsequent cold tolerance.

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Methyl jasmonate mediates melatonin-induced cold tolerance of grafted watermelon plants

Cited by 100 publications

References 49 publications

Melatonin-induced cold and drought tolerance requires brassinosteroids and hydrogen peroxide signaling in perennial ryegrass

Melatonin-induced cold and drought tolerance requires brassinosteroids and hydrogen peroxide signaling in perennial ryegrass

McMYB4 improves temperature adaptation by regulating phenylpropanoid metabolism and hormone signaling in apple

Positive Interaction between H2O2 and Ca2+ Mediates Melatonin-Induced CBF Pathway and Cold Tolerance in Watermelon (Citrullus lanatus L.)

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