Methyl Jasmonate Induces Genes Involved in Linalool Accumulation and Increases the Content of Phenolics in Two Iranian Coriander (Coriandrum sativum L.) Ecotypes

Abstract

The medicinal herb coriander (Coriandrum sativum L.), with a high linalool (LIN) content, is widely recognized for its therapeutic benefits. As a novel report, the goals of this study were to determine how methyl jasmonate (MeJA) affects total phenolic content (TPC), LIN content, flavonoid content (TFC), and changes in gene expression involved in the linalool biosynthesis pathway (CsγTRPS and CsLINS). Our findings showed that, in comparison to the control samples, MeJA treatment substantially enhanced the TPC, LIN, and TFC content in both ecotypes. Additionally, for both Iranian coriander ecotypes, treatment-induced increases in CsγTRPS and CsLINS expression were connected to LIN accumulation in all treatments. A 24 h treatment with 150 µM MeJA substantially increased the LIN content in the Mashhad and Zanjan ecotypes, which was between 1.48 and 1.69 times greater than that in untreated plants, according to gas chromatography–mass spectrometry (GC-MS) analysis. Our findings demonstrated that MeJA significantly affects the accumulation of LIN, TPC, and TFC in Iranian C. sativum treated with MeJA, which is likely the consequence of gene activation from the monoterpene biosynthesis pathway. Our discoveries have improved the understanding of the molecular mechanisms behind LIN synthesis in coriander plants.

Molecular and Physiological Evaluation of Bread Wheat (Triticum aestivum L.) Genotypes for Stay Green under Drought Stress

“…The reaction was terminated by heating at 70 °C for 5 min, and the synthesized cDNA was stored at −20–42 °C for RT–PCR analysis. The whole process of RNA extraction and cDNA synthesis was accomplished according to the extraction and cDNA synthesis discussed in Kianersi et al [ 42 ].…”

Intraspecific divergence in essential oil content, composition and genes expression patterns of monoterpene synthesis in Origanum vulgare subsp. vulgare and subsp. gracile under salinity stress

“…Also, the highest expression of the studied genes and carvacrol content was observed in vulgare subspecies at 50 mM salt stress. The gene expression levels may be variable depending on the stress and plant species [ 69 ]. The higher expression of these genes in retort to moderate salinity stress may reverberate the elevation of phenolic monoterpenes such as carvacrol.…”

Coriander (Coriandrum sativum) Polyphenols and Their Nutraceutical Value against Obesity and Metabolic Syndrome

“…It has been shown that the preharvest treatment of coriander leaves with malic, oxalic and acetylsalicylic acids increases the polyphenolic content and hence the antioxidant activity of coriander extract [40]. Kianersi et al (2022) [41] report that a notable plant hormone, methyl jasmonate (MeJA), has been shown to impact the accumulation of these secondary metabolites by modulating signal transduction and defence gene expression. The study by Kianersi et al (2022) [42] further investigates the effects of MeJA on the expression of key genes involved in linalool and γ-terpinene biosynthesis, phenolic compound accumulation and linalool content in two Iranian Coriandrum sativum genotypes (Mashhad and Zanjan).…”

“…Kianersi et al (2022) [41] report that a notable plant hormone, methyl jasmonate (MeJA), has been shown to impact the accumulation of these secondary metabolites by modulating signal transduction and defence gene expression. The study by Kianersi et al (2022) [42] further investigates the effects of MeJA on the expression of key genes involved in linalool and γ-terpinene biosynthesis, phenolic compound accumulation and linalool content in two Iranian Coriandrum sativum genotypes (Mashhad and Zanjan). The study results indicate that MeJA treatment enhances the expression of the linalool synthase (CsLINS) and γ-terpinene synthase (CsγTRPS) genes, leading to the increased biosynthesis of linalool and total phenolic content in the two genotypes.…”