Methyl jasmonate-induced accumulation of metabolites and transcriptional responses involved in triterpene biosynthesis in Siraitia grosvenorii fruit at different growing stages

Zhang, Kailun; Luo, Zuliang; Guo, Yu‐Hua; Mo, Changming; Tu, Dongping; Ma, Xiaojun; Bai, Liang

doi:10.5586/asbp.3503

Cited by 8 publications

(5 citation statements)

References 48 publications

(60 reference statements)

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“…Further analysis revealed that Cu in the soil was significantly correlated with the ginsenosides content and the activity of CYP450 in ginseng of different plant ages, suggesting that Cu has a positive effect on ginseng growth [ 10 ]. These results were consistent with the important role of copper in CYP450, which could activate the CYP450 enzyme [ 37 , 38 ]. Moreover, a linear relationship suggested that heavy metal content in soil could predict ginsenosides, Cu and As content in ginseng.…”

Section: Discussionsupporting

confidence: 87%

Ginseng of different ages is affected by the accumulation of heavy metals in ginseng soil

et al. 2022

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Heavy-metal pollution has been established to affect ginseng quality. However, this effect is still unknown in ginseng of different ages, emphasizing the need to investigate the effects of heavy metals in soils on ginseng growth. Herein, we determined the content of heavy metals (Cu, Cd, Pb, Hg, and As) in ginseng of different ages (2 to 6-year-old) and the corresponding soil samples. Then, the total ginsenosides content of ginseng and rate-limiting enzyme (HMGR, SQE, CYP450) activity in the synthesis of ginsenosides were assessed. Results from 200 differently-aged Chinese ginseng showed that increased ginsenoside content in 3 to 5-year-old ginseng was paralleled by increased heavy metal element content in ginseng and its soil. The activity of rate-limiting enzymes increased in the first four years of ginseng growth and then exhibited a steady or downward trend. Further analysis suggested that heavy metal elements in soils could directly affect ginsenoside content. Moreover, we found that Cu significantly affected the rate-limiting enzyme CYP450 activity. Further principal component analysis and correlation analysis found that heavy metals could obviously inhibit ginseng growth during the 5th and 6th years. Heavy metal content in soils has huge prospects for predicting ginsenoside, Cu and As content in ginseng. This study provided support for ginseng cultivation, quality research and quality assessment.

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Section: Discussionsupporting

confidence: 87%

Ginseng of different ages is affected by the accumulation of heavy metals in ginseng soil

et al. 2022

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“…It has been found that exogenous plant hormone, like methyl jasmonate, abscisic acid, and salicylic acid, could affect the accumulation of secondary metabolites in Salvia miltiorrhiza [30], Glycyrrhiza uralensis root [31], and Uncaria tomentosa [32]. Exogenous plant hormone applied in the S. grosvenorii might be an appropriate approach for good production, and Zhang et al demonstrated that methyl jasmonate applied in different development stages of S. grosvenorii fruit significantly promotes the accumulation of squalene, cucurbitadienol, and mogrosides IIE [33].…”

Section: Discussionmentioning

confidence: 99%

“…Three intermediates (squalene, cucurbitadienol, and cycloartenol) were measured. The fruit samples for GC-MS analysis were prepared and the basic operational conditions were as described previously [13,33]. Helium gas was used as the carrier gas, and the carrier flow rate was 1.5 mL min −1 .…”

Section: Methodsmentioning

confidence: 99%

“…Twenty-four structural genes in the mogroside biosynthetic pathway were measured by qRT-PCR. Total RNA extraction, quality and concentration assessment, and the expression profiles of the 24 structural genes determined using the BIO-RAD CFX system (BIO-RAD, USA) were performed as described previously [33]. First-strand cDNA was synthesized by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (Transgen Biotech, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

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Effects of Forchlorfenuron on the Morphology, Metabolite Accumulation, and Transcriptional Responses of Siraitia grosvenorii Fruit

et al. 2019

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Siraitia grosvenorii fruit, called luo-han-guo (LHG), have been used as a traditional Chinese medicine (TCM) and dietary supplements for many years. Mogrosides, the main bioactive ingredients in LHG, are commercially available worldwide as a non-sugar-based and noncaloric sweetener. However, the production cannot meet the increasing market demand because of the low content of mogrosides and the small size of LHG. Therefore, some advanced technologies have been applied for improving the quality of LHG. Forchlorfenuron (CPPU), a plant growth regulator, is widely applied to promote plant yield and the secondary metabolite synthesis. Here, the content of nine mogrosides and three intermediates in LHG that were treated with three different concentrations of CPPU were determined by LC-MS/MS and GC-MS, respectively. The total content of mogrosides in LHG treated with CPPU was not enhanced, and the proportion of some main bioactive ingredients, including mogroside V (MV), were decreased relative to that of the control treatment. Morphological and cytological observations showed CPPU could make an early lignification in fruit epidermal cells, and 5 or 25 mg L−1 CPPU could inhibit LHG growth. The expression levels of 24 key genes in the mogroside biosynthesis pathway were measured and revealed that genes downregulated in upstream, and different expressions of SgUGTs would affect the accumulations and proportions of mogrosides in LHG induced by CPPU. This was the first study that applied CPPU individually on LHG, and assessed effects of CPPU on the morphology, the accumulation of metabolites, and expression profiles of 24 structural genes. The CPPU effects on LHG were undesirable, including development inhibition and the decrease of main mogroside content. These will provide guidance for the rational application of CPPU.

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“…Based on the gene annotation of the transcriptome, 104 unigenes were identified and their responses to MeJA induction were investigated involved in the biosynthesis of indole, terpenoid and phenylpropanoid [ 30 ]. The results showed that transcriptional levels of SgHMGR , SgSQS , SgCS and SgCYP450 were up-regulated and their responses in the presence of MeJA were related to the concentration and timing of MeJA treatment in Siraitia grosvenorii [ 31 ]. MeJA-regulated rubber biosynthesis, based on a differential expression analysis, showed that the expression of HMGCR , FPPS , IDI , GGPPS , REF/SRPP and transcription factors (bHLH, MYB, AP2/EREBP and WRKY) increased with MeJA treatment in TKS [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals Differentially Expressed Genes That Regulate Biosynthesis of the Active Compounds with Methyl Jasmonate in Rosemary Suspension Cells

Yao

Zhang

Chen

et al. 2021

Genes

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To study the effects of Methyl jasmonates (MeJA) on rosemary suspension cells, the antioxidant enzymes’ change of activities under different concentrations of MeJA, including 0 (CK), 10 (M10), 50 (M50) and 100 μM MeJA (M100). The results demonstrated that MeJA treatments increased the activities of phenylalanine ammonla-lyase (PAL), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and polyphenol oxidase (PPO) and reduced the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA), thus accelerating the ROS scavenging. Comparative transcriptome analysis of different concentrations of MeJA showed that a total of 7836, 6797 and 8310 genes were differentially expressed in the comparisons of CKvsM10, CKvsM50, CKvsM100, respectively. The analysis of differentially expressed genes (DEGs) showed phenylpropanoid biosynthesis, vitamin B6, ascorbate and aldarate metabolism-related genes were significantly enriched. The transcripts of flavonoid and terpenoid metabolism pathways and plant hormone signal transduction, especially the jasmonic acid (JA) signal-related genes, were differentially expressed in CKvsM50 and CKvsM100 comparisons. In addition, the transcription factors (TFs), e.g., MYC2, DELLA, MYB111 played a key role in rosemary suspension cells under MeJA treatments. qRT-PCR of eleven DEGs showed a high correlation between the RNA-seq and the qRT-PCR result. Taken together, MeJA alleviated peroxidative damage of the rosemary suspension cells in a wide concentration range via concentration-dependent differential expression patterns. This study provided a transcriptome sequence resource responding to MeJA and a valuable resource for the genetic and genomic studies of the active compounds engineering in rosemary.

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Methyl jasmonate-induced accumulation of metabolites and transcriptional responses involved in triterpene biosynthesis in Siraitia grosvenorii fruit at different growing stages

Cited by 8 publications

References 48 publications

Ginseng of different ages is affected by the accumulation of heavy metals in ginseng soil

Ginseng of different ages is affected by the accumulation of heavy metals in ginseng soil

Effects of Forchlorfenuron on the Morphology, Metabolite Accumulation, and Transcriptional Responses of Siraitia grosvenorii Fruit

Transcriptome Analysis Reveals Differentially Expressed Genes That Regulate Biosynthesis of the Active Compounds with Methyl Jasmonate in Rosemary Suspension Cells

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