Methyl Jasmonate Increases Isoflavone Production in Soybean Cell Cultures by Activating Structural Genes Involved in Isoflavonoid Biosynthesis

Abstract

Isoflavonoids are a class of biologically active natural products that accumulate in soybean ( Glycine max L.) seeds during development, play vital roles in plant defense, and act as phytoestrogens with important human health benefits. Plant cell suspension cultures represent an excellent source of biologically important secondary metabolites. We found that methyl jasmonate (MJ) treatment increased isoflavone production in soybean suspension cell cultures. To investigate the underlying mechanism, we examined the expression of structural genes ( CHS6, CHS7, CHI1, IFS1, IFS2, IFMaT, and HID) in the isoflavonoid biosynthesis pathways in soybean suspension cells under various abiotic stress conditions. MJ treatment had the most significant effect on gene expression and increased the production of three glycosidic isoflavones (daidzin, malonyldaidzin, and malonylgenistin), with the maximum total isoflavone production (∼10-fold increase) obtained on day 9 after MJ application. MJ treatment significantly increased total phenolic contents and upregulated isoflavonoid biosynthesis genes, shedding light on the underlying mechanism.

Induced extracellular production of viniferins in grapevine cell culture medium by elicitation with methyl jasmonate and stevioside

“…Cell suspension cultures were sub-cultured into 100 mL MS1D liquid medium in 500 mL Erlenmeyer flasks and placed on a rotary shaker (90 rpm) at 24°C under dark conditions for 7 days as described by Jeong et al (2018). For RT-PCR analysis, pre-cultured cells (6 mL aliquots) were transferred into each well of a 6-well tissue plate.…”

“…Cell viability was monitored by staining cell suspensions with 0.1% Evans blue (Sigma-Aldrich) as previously described by Jeong et al (2018).…”

“…Total RNA isolation, cDNA synthesis, and RT-PCR were performed as previously described (An et al 2015;Jeong et al 2018). The beta-actin (VvActin7, XM_002282480.4) gene in grapevines was used as an internal control in RT-PCR.…”

“…The bio-production of resveratrol and its derivatives in grapevine cell cultures has been reported as a promising biotechnological alternative to their plant extraction and chemical synthesis. Plant cell cultures have been used extensively for bio-production of valuable secondary metabolites under controlled conditions independent of climatic changes or soil conditions (Giri and Zaheer 2016;Hussain et al 2012;Jeandet et al 2016;Jeong et al 2018;Mulabagal and Tsay 2004;Murthy et al 2014). To date, elicitation with MJ combined with dimethyl-b-cyclodextrins (CD-M) has proven the most efficient approach for high production of resveratrol and its derivatives in grapevine cell cultures (Bru et al 2006;Lijavetzky et al 2008;Martinez-Esteso et al 2009;Martínez-Márquez et al 2016) (Donnez et al 2009;Lambert et al 2019).…”

Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum

“…Jasmonic acid (JA) and its derivative, methyl jasmonate (MeJA), are hormonal cues released by plants that signal defense responses to curb damage from biotic and abiotic stresses [16]. In plants, JA and its derivatives are involved in the regulation of many developmental processes, including male fertility [17], fruit ripening [18], and root growth [19].…”