Methyl jasmonate enhances phlorotannin production in the brown seaweed Ecklonia cava

Chowdhury, Muhammad Tanvir Hossain; Cho, Ji-Young; Ahn, Dong-Hyun; Hong, Yong‐Ki

doi:10.1007/s10811-014-0456-6

Cited by 5 publications

(4 citation statements)

References 32 publications

(35 reference statements)

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“…The timing and magnitude of the induced increase in phlorotanin concentration was similar to those caused by real or simulated herbivory, indicating the putative role of MeJA in antiherbivore responses in algae. Recently, Chowdhury et al (2014) also showed that the addition of 2 mM MeJA for 24 h to post-harvest cultures of Ecklonia cava increased the crude phlorotanin (brown algal polyphenol) content by 156%. In contrast, Collén et al (2006) found that only the transcripts for DHAP (3-deoxy-D-arabino-heptulosonic acid-7-phosphate) synthase in a C. crispus microarray was overexpressed at 6 h, while no other transcripts involved in the shikimate pathway were identified.…”

Section: Discussionmentioning

confidence: 99%

Methyl Jasmonate-Induced Lipidomic and Biochemical Alterations in the Intertidal MacroalgaGracilaria dura(Gracilariaceae, Rhodophyta)

2015

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The role of exogenously added methyl jasmonate (MeJA), a lipid-derived signaling compound, in inducing oxidative stress in the marine red macroalga Gracilaria dura was investigated. MeJA at a concentration of 1-100 µM was a strong stimulant of reactive oxygen species (H(2)O(2), HO· and O(2) (·-)) (P < 0.05) causing considerable oxidative stress in G. dura. This further led to lipid peroxidation and degradation of the pigments Chl a and phycocyanin, with a concomitant increase in phycoerythrin. The MeJA-induced oxidative burst also led to the induction of a fatty acid oxidation cascade, resulting in the synthesis of hydroxy-oxylipins and the up-regulation of the 13-lipoxygenase pathway. Electrospray ionization-mass spectrometry-based shotgun lipidomic analysis revealed that monogalactosyldiacylglycerol (a chloroplastic glycerolipid) and phosphatidylcholine (extrachloroplastidic phopholipid) were the most affected lipid classes. The degradation of 18:3-fatty acid-containing monogalactosyldiacylglycerol inferred that it provided fatty acyl chains for the biosynthesis of 13-hydroperoxylinolenic acid, which was further directed towards either the jasmonate pathway or other alternative pathways of the fatty acid oxidation cascade, analogous to higher plants. Also, G. dura modulated the lipid acyl chains in such a way that no significant change was observed in the fatty acid profile of the treated thalli as compared with those of the control, except for C16:0, C16:1 (n-9), C20:3 (n-6) and C20:4 (n-6) (P < 0.05). Furthermore, MeJA caused the accumulation of phenolic compounds and the up-regulation of enzymes involved in secondary metabolism such as polyphenol oxidase, shikimate dehydrogenase and phenylalanine ammonia-lyase, indicating a shift towards secondary metabolism as a defense strategy to combat the induced oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Methyl Jasmonate-Induced Lipidomic and Biochemical Alterations in the Intertidal MacroalgaGracilaria dura(Gracilariaceae, Rhodophyta)

2015

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“…Isolation of dieckol, phlorotannin 974B, phlorotannin 974A, and phlorofucofuroeckol-A from ethyl ether fraction of crude extract [188].…”

Section: Luna-c18 (-)mentioning

confidence: 99%

“…Reversed-phase (RP) HPLC columns have been the most used in macroalgae phenolic rich extracts [32,39,62,73,157,159,169,173,180,182,[186][187][188][189]. Octadecyl ("C18" or "ODS") has been the most used stationary phase, with a particle size of 5 µm.…”

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confidence: 99%

“…Elution of these molecules from reversed phases has been performed using either water, methanol, or acetonitrile in different combinations. Although water to acetonitrile has been the most frequently used eluent, water to methanol gradients have been also used with success [21,157,187,188], which is an advantage since methanol is relatively easy to evaporate, and is much cheaper than acetonitrile. Nonetheless, acetonitrile might provide greater resolution at lower percentages, and might be adequate to use in columns of lower maximum pressures [183].…”

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The Quest for Phenolic Compounds from Macroalgae: A Review of Extraction and Identification Methodologies

et al. 2019

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The current interest of the scientific community for the exploitation of high-value compounds from macroalgae is related to the increasing knowledge of their biological activities and health benefits. Macroalgae phenolic compounds, particularly phlorotannins, have gained particular attention due to their specific bioactivities, including antioxidant, antiproliferative, or antidiabetic. Notwithstanding, the characterization of macroalgae phenolic compounds is a multi-step task, with high challenges associated with their isolation and characterization, due to the highly complex and polysaccharide-rich matrix of macroalgae. Therefore, this fraction is far from being fully explored. In fact, a critical revision of the extraction and characterization methodologies already used in the analysis of phenolic compounds from macroalgae is lacking in the literature, and it is of uttermost importance to compile validated methodologies and discourage misleading practices. The aim of this review is to discuss the state-of-the-art of phenolic compounds already identified in green, red, and brown macroalgae, reviewing their structural classification, as well as critically discussing extraction methodologies, chromatographic separation techniques, and the analytical strategies for their characterization, including information about structural identification techniques and key spectroscopic profiles. For the first time, mass spectrometry data of phlorotannins, a chemical family quite exclusive of macroalgae, is compiled and discussed.

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Physiological acclimation of Lessonia spicata to diurnal changing PAR and UV radiation: differential regulation among down-regulation of photochemistry, ROS scavenging activity and phlorotannins as major photoprotective mechanisms

et al. 2016

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Intertidal macroalgae are constantly subjected to high variations in the quality and quantity of incident irradiance that can eventually generate detrimental effect on the photosynthetic apparatus. The success of these organisms to colonize the stressful coastal habitat is mainly associated with the complexity of their morphological structures and the efficiency of the anti-stress mechanisms to minimize the physiological stress. Lessonia spicata (Phaeophyceae), a brown macroalga, that inhabits the intertidal zone in central-southern Chile was studied in regard to their physiological (quantum yield, electron transport rate, pigments) and biochemical (phlorotannins content, antioxidant metabolism, oxidative stress) responses during a daily light cycle under natural solar radiation. Major findings were that F /F, photosynthetic parameters (ETR, alpha, E ) and pigments in L. spicata showed an inverse relationship to the diurnal changes in solar radiation. Phlorotannins levels and antioxidant activity showed their highest values in treatment that included UV radiation. There was an increase in SOD and APX in relation at light stress, with a peak in activity between 5.2 and 10.1 W m of biologically effective dose. The increase in peroxidative damage was proportional to light dose. These results indicated that different light doses can trigger a series of complementary mechanisms of acclimation in L. spicata based on: (i) down-regulation of photochemistry activity and decrease in concentration of photosynthetic pigments; (ii) induction of phenolic compounds with specific UV-screening functions; and (iii) reactive oxygen species (ROS) scavenging activity via complementary repair of the oxidative damage through increased activity of antioxidant enzymes and potentially increased amounts of phenolic compounds.

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Methyl jasmonate enhances phlorotannin production in the brown seaweed Ecklonia cava

Cited by 5 publications

References 32 publications

Methyl Jasmonate-Induced Lipidomic and Biochemical Alterations in the Intertidal MacroalgaGracilaria dura(Gracilariaceae, Rhodophyta)

Methyl Jasmonate-Induced Lipidomic and Biochemical Alterations in the Intertidal MacroalgaGracilaria dura(Gracilariaceae, Rhodophyta)

The Quest for Phenolic Compounds from Macroalgae: A Review of Extraction and Identification Methodologies

Physiological acclimation of Lessonia spicata to diurnal changing PAR and UV radiation: differential regulation among down-regulation of photochemistry, ROS scavenging activity and phlorotannins as major photoprotective mechanisms

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