Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and non-enzymatic antioxidant in suspension culture Panax ginseng roots in bioreactors

Abstract

The effects of methyl jasmonate (MJ) and salicylic acid (SA) on changes of the activities of major antioxidant enzymes, superoxide anion accumulation (O2-), ascorbate, total glutathione (TG), malondialdehyde (MDA) content and ginsenoside accumulation were investigated in ginseng roots (Panax ginseng L.) in 4 l (working volume) air lift bioreactors. Single treatment of 200 microM MJ and SA to P. ginseng roots enhanced ginsenoside accumulation compared to the control and harvested 3, 5, 7 and 9 days after treatment. MJ and SA treatment induced an oxidative stress in P. ginseng roots, as shown by an increase in lipid peroxidation due to rise in O2- accumulation. Activity of superoxide dismutase (SOD) was inhibited in MJ-treated roots, while the activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), SOD, guaiacol peroxidase (G-POD), glutathione peroxidase (GPx) and glutathione reductase (GR) were induced in SA-treated roots. A strong decrease in the activity of catalase (CAT) was obtained in both MJ- and SA-treated roots. Activities of ascorbate peroxidase (APX) and glutathione S transferase (GST) were higher in MJ than SA while the contents of reduced ascorbate (ASC), redox state (ASC/(ASC+DHA)) and TG were higher in SA- than MJ-treated roots while oxidized ascorbate (DHA) decreased in both cases. The result of these analyses suggests that roots are better protected against the O2- stress, thus mitigating MJ and SA stress. The information obtained in this work is useful for efficient large-scale production of ginsenoside by plant-root cultures.

daptation of the in vitro Culture of Origanum majorana L. For Production of Phenolic Acids

“…Copper ion significantly inhibited Panax ginseng root growth in a bioreactor and resulted in an increase in protein oxidation (21%) which was measured as carbonyl content at concentration of 50 μM. In addition, Cu 2+ stress resulted in increased activities of glucose-6-phosphate dehydrogenase (G6PDH), shikimate dehydrogenase (SKDH), PAL and cinnamyl alcohol dehydrogenase (CAD) [49]. The induced activities of substrate specific peroxidases (caffeic acid peroxidase, chlorogenic acid peroxidase, polyphenol oxidase and β-glucosidase) indicated that these enzymes play an important role in the synthesis of phenolic compounds.…”

Methyl Jasmonate and Salicylic Acid Induced Oxidative Stress and Accumulation of Phenolics in Panax ginseng Bioreactor Root Suspension Cultures

“…Stock roots of P. ginseng were maintained in Murashighe and Skoog medium (MS, [32]] supplemented with indolebutyric acid (IBA, 5.0 mg L -1 ), kinetin (0.5 mg L -1 ) and sucrose (5%) and cultured as described by Ali et al [13]. These proliferated roots were used as explants and named as Chungbuk National University Line 1 (CBN-1) for further experiments.…”

“…Exogenous application of methyl jasmonate and/or salicylic acid could induce the expression of many defense genes in different plants [11,12]. Our previous studies have shown that exogenous application of MJ and SA increases ginsenoside content in P. ginseng [13]. Phenolic compounds are ubiquitous in all plant organs and are, therefore, an integral part of the human diet.…”

Effect of Shading, Light Quality, and Chemical Elicitation on Growth and Bioactive Compound Content of Potentilla kleiniana Wight et Arnott

“…그러나 식물체의 생리활성물질은 재 배환경, 수확시기 등에 따라 함량 차이가 크며 신약, 화장품, 건 강식품 소재 등 산업적으로 사용하기 위해서는 연중 생산과 생 리활성물질 변화가 적은 원료를 효율적으로 생산하는 시스템의 확립이 필요하다 (Southwell and Bourke, 2001). (Ali et al, 2006;Savitha et al, 2006). 특히 abiotic elicitor 중, salicylic acid (SA)와 methyl jasmonate (MeJA)는 식물체에서 생리활성물질의 생합성을 유도하는 신호물질로 알려져 있다 (Chen and Chen, 1999;Creelman and Mullet, 1997).…”