Methyl jasmonate affects phenolic metabolism and gene expression in blueberry (<i>Vaccinium corymbosum</i>)

Cocetta, Giacomo; Rossoni, Mara; Gardana, Claudio; Mignani, I.; Ferrante, António; Spinardi, A.

doi:10.1111/ppl.12243

Cited by 53 publications

(34 citation statements)

References 58 publications

(65 reference statements)

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“…In fact, there is evidence indicating that MeJA treatments increment anthocyanin accumulation in strawberries [48] and apple fruits [49]. Interestingly, Cocetta et al [50] detected an increased expression of phenylalanine ammonium lyase, chalcone synthase, and anthocyanidin synthase genes in blueberry plants, as well as increments of epicatechin and anthocyanin concentrations in plants of blueberry treated with MeJA. In lettuce, the pre-harvest exogenous application of methyl jasmonate (MeJA) increased total phenolic compound content and antioxidant capacity, which was attributed to a caffeic acid derivative and an unknown phenolic compound [27].…”

Section: Discussionmentioning

confidence: 99%

The Anti-Proliferative and Anti-Invasive Effect of Leaf Extracts of Blueberry Plants Treated with Methyl Jasmonate on Human Gastric Cancer In Vitro Is Related to Their Antioxidant Properties

et al. 2020

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Gastric cancer is the third main cause of cancerous tumors in humans in Chile. It is well-accepted that a diet rich in antioxidant plants could help in fighting cancer. Blueberry is a fruit crop with a high content of antioxidants. Methyl jasmonate (MeJA) is a phytohormone involved in plant defenses under stress conditions. The exogenous application of MeJA can improve the antioxidant properties in plants. We studied in vitro and in vivo anticancer action on human gastric cancer (cell line AGS) and the antioxidant properties of extracts from blueberry plants untreated and treated with MeJA. The results demonstrated that leaf extracts displayed a higher inhibition of cancer cell viability as well as greater antioxidant properties compared to fruit extracts. Besides, MeJA applications to plants improved the antioxidant properties of leaf extracts (mainly anthocyanins), increasing their inhibition levels on cell viability and migration. It is noteworthy that leaf extract from MeJA-treated plants significantly decreased cancer cell migration and expression of gastric cancer-related proteins, mainly related to the mitogen-activating protein kinase (MAPK) pathway. Interestingly, in all cases the anticancer and antioxidant properties of leaf extracts were strongly related. Despite highlighted outcomes, in vivo results did not indicate significant differences in Helicobacter pylori colonization nor inflammation levels in Mongolian gerbils unfed and fed with blueberry leaf extract. Our findings demonstrated that MeJA increased antioxidant compounds, mainly anthocyanins, and decreased the viability and migration capacity of AGS cells. In addition, leaf extracts from MeJA-treated plants were also able to decrease the expression of gastric cancer-related proteins. Our outcomes also revealed that the anthocyanin-rich fraction of blueberry leaf extracts showed higher in vitro antiproliferative and anti-invasive effects than the crude leaf extracts. However, it is still uncertain whether the leaf extracts rich in anthocyanins of blueberry plants are capable of exerting a chemopreventive or chemoprotective effect against gastric cancer on an in vivo model.

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Section: Discussionmentioning

confidence: 99%

The Anti-Proliferative and Anti-Invasive Effect of Leaf Extracts of Blueberry Plants Treated with Methyl Jasmonate on Human Gastric Cancer In Vitro Is Related to Their Antioxidant Properties

et al. 2020

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“…Sucrose and total sugars were assayed according to the resorcinol method and anthrone assay, respectively (Cocetta et al, 2015). Absorbance was read at 500 nm for sucrose and at 620 nm for total sugars and the levels were calculated referring to sucrose or glucose calibration curves, respectively.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Borage Extracts As Potential Biostimulant Using a Phenomic, Agronomic, Physiological, and Biochemical Approach

et al. 2017

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Biostimulants are substances able to improve water and nutrient use efficiency and counteract stress factors by enhancing primary and secondary metabolism. Premise of the work was to exploit raw extracts from leaves (LE) or flowers (FE) of Borago officinalis L., to enhance yield and quality of Lactuca sativa ‘Longifolia,’ and to set up a protocol to assess their effects. To this aim, an integrated study on agronomic, physiological and biochemical aspects, including also a phenomic approach, has been adopted. Extracts were diluted to 1 or 10 mL L–1, sprayed onto lettuce plants at the middle of the growing cycle and 1 day before harvest. Control plants were treated with water. Non-destructive analyses were conducted to assess the effect of extracts on biomass with an innovative imaging technique, and on leaf photosynthetic efficiency (chlorophyll a fluorescence and leaf gas exchanges). At harvest, the levels of ethylene, photosynthetic pigments, nitrate, and primary (sucrose and total sugars) and secondary (total phenols and flavonoids) metabolites, including the activity and levels of phenylalanine ammonia lyase (PAL) were assessed. Moreover, a preliminary study of the effects during postharvest was performed. Borage extracts enhanced the primary metabolism by increasing leaf pigments and photosynthetic activity. Plant fresh weight increased upon treatments with 10 mL L–1 doses, as correctly estimated by multi-view angles images. Chlorophyll a fluorescence data showed that FEs were able to increase the number of active reaction centers per cross section; a similar trend was observed for the performance index. Ethylene was three-fold lower in FEs treatments. Nitrate and sugar levels did not change in response to the different treatments. Total flavonoids and phenols, as well as the total protein levels, the in vitro PAL specific activity, and the levels of PAL-like polypeptides were increased by all borage extracts, with particular regard to FEs. FEs also proved efficient in preventing degradation and inducing an increase in photosynthetic pigments during storage. In conclusion, borage extracts, with particular regard to the flower ones, appear to indeed exert biostimulant effects on lettuce; future work will be required to further investigate on their efficacy in different conditions and/or species.

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“…& et al, 2007). Moreover, phenols found a large quantities in plants and have antioxidant activity (Cocetta, G. & et al, 2015). Large inhibition zone found in E.corona followed by K.phenumonia, while no inhibition indicate in P. bulgaris.…”

Section: Resultsmentioning

confidence: 99%

Detection of Total Flavenoids, Reductive Ability, and Anti-microbial in Glycyrrhiza and Achillea Medicinal Plants

Al-Ezzy¹,

Al-Jumaili²,

Al-Ani³

2017

JBLS

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According to WHO, 80% of the medicine in the global market are from plants. The Glycyrrhiza glabra and Achillea mellofolium are very promising plants. Three experiments were done for these plants. The total flavenoids, was higher in G.glabra than A.mellofolium. In the second experiment, reductive ability was very effective in scavenger the free radical specially, in high doses. In the third experiment, the antimicrobial of the three types of bacteria isolated from two sources assess using extracts from plants.

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Methyl jasmonate affects phenolic metabolism and gene expression in blueberry (Vaccinium corymbosum)

Cited by 53 publications

References 58 publications

The Anti-Proliferative and Anti-Invasive Effect of Leaf Extracts of Blueberry Plants Treated with Methyl Jasmonate on Human Gastric Cancer In Vitro Is Related to Their Antioxidant Properties

The Anti-Proliferative and Anti-Invasive Effect of Leaf Extracts of Blueberry Plants Treated with Methyl Jasmonate on Human Gastric Cancer In Vitro Is Related to Their Antioxidant Properties

Evaluation of Borage Extracts As Potential Biostimulant Using a Phenomic, Agronomic, Physiological, and Biochemical Approach

Detection of Total Flavenoids, Reductive Ability, and Anti-microbial in Glycyrrhiza and Achillea Medicinal Plants

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