Methyl Gallate Inhibits the Production of Interleukin-6 and Nitric Oxide via Down-Regulation of Extracellular-Signal Regulated Protein Kinase in RAW 264.7 Cells

Chae, Hee‐Sung; Kang, Ok‐Hwa; Choi, Jang‐Gi; Oh, You-Chang; Lee, Young‐Seob; Brice, Obiang-Obounou; Chong, Myong-Soo; Lee, Ki-Nam; Shin, Dong‐Won; Kwon, Dong‐Yeul

doi:10.1142/s0192415x10008391

Cited by 33 publications

(22 citation statements)

References 21 publications

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“…In addition to ethyl gallate, Galla Rhois contains several gallic acid-derived components possessing antibacterial (Choi et al, 2009) and antitumor properties . A recent study has demonstrated that methyl gallate derived from ethanol extract of Galla Rhois inhibits the production of nitric oxide and interleukin-6 (Chae et al, 2010), suggesting that methyl gallate from Galla Rhois possesses potent anti-inflammatory activities. It would be interesting to investigate the inhibitory effect of ethyl gallate and methyl gallate on the inflammatory responses, which gives us valuable information about what component from Galla Rhois would be more effective for the treatment of inflammation-related diseases.…”

Section: Discussionmentioning

confidence: 99%

Involvement of heme oxygenase-1 induction in inhibitory effect of ethyl gallate isolated from Galla Rhois on nitric oxide production in RAW 264.7 macrophages

et al. 2011

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In the present study, we investigated an anti-inflammatory effect of ethyl gallate (EG) isolated from Galla Rhois as evaluated by inhibition of nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression, and a potential role of heme oxygenase-1 (HO-1) in the inhibition of NO production elicited by EG. Treatment of RAW264.7 macrophages with EG significantly inhibited the production of NO and iNOS expression stimulated by lipopolysaccharide (LPS). We also demonstrated that EG treatment increased HO-1 mRNA and protein expression, as assessed by quantitative RT-PCR and Western blot analysis. EG treatment also increased the levels of nuclear factor-erythroid 2-related factor 2, which is critical for transcriptional induction of HO-1. In addition, treatment with SnPP (tin protoporphyrin IX), a selective HO-1 inhibitor, counteracted the inhibitory effect of EG on nitrite production, suggesting that HO-1 is, at least in part, implicated in the inhibition of NO production induced by EG treatment. Taken together, these results indicate that EG isolated from Galla Rhois suppresses NO production in LPS-stimulated RAW 264.7 macrophages via HO-1 induction.

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Section: Discussionmentioning

confidence: 99%

Involvement of heme oxygenase-1 induction in inhibitory effect of ethyl gallate isolated from Galla Rhois on nitric oxide production in RAW 264.7 macrophages

et al. 2011

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“…The latter is a phytochemical and polyphenolic compound that is widely believed to have medicinal properties such as antioxidant [19], antitumor [20], anti-inflammatory [21], and antimicrobial [22]. In addition, it has been proven that methyl gallate acts as a potent inhibitor of sodium and potassium channels in skeletal muscle cells [23].…”

Section: Introductionmentioning

confidence: 99%

Methyl Gallate Inhibits Osteoclast Formation and Function by Suppressing Akt and Btk-PLCγ2-Ca2+ Signaling and Prevents Lipopolysaccharide-Induced Bone Loss

Baek

Kim

Lee

et al. 2017

IJMS

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Abstract:In the field of bone research, various natural derivatives have emerged as candidates for osteoporosis treatment by targeting abnormally elevated osteoclastic activity. Methyl gallate, a plant-derived phenolic compound, is known to have numerous pharmacological effects against inflammation, oxidation, and cancer. Our purpose was to explore the relation between methyl gallate and bone metabolism. Herein, we performed screening using methyl gallate by tartrate resistant acid phosphatase (TRAP) staining and revealed intracellular mechanisms responsible for methyl gallate-mediated regulation of osteoclastogenesis by Western blotting and quantitative reverse transcription polymerase chain reaction (RT-PCR). Furthermore, we assessed the effects of methyl gallate on the characteristics of mature osteoclasts. We found that methyl gallate significantly suppressed osteoclast formation through Akt and Btk-PLCγ2-Ca 2+ signaling. The blockade of these pathways was confirmed through transduction of cells with a CA-Akt retrovirus and evaluation of Ca 2+ influx intensity (staining with Fluo-3/AM). Indeed, methyl gallate downregulated the formation of actin ring-positive osteoclasts and resorption pit areas. In agreement with in vitro results, we found that administration of methyl gallate restored osteoporotic phenotype stimulated by acute systemic injection of lipopolysaccharide in vivo according to micro-computed tomography and histological analysis. Our data strongly indicate that methyl gallate may be useful for the development of a plant-based antiosteoporotic agent.

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“…Thus, administration of gallic acid to rats (Mohamed & Abd El‐Twab, ) and mice (Pandurangan et al, ) caused inhibition of iNOS expression and decreased NO levels, which is accompanied by free radical scavenging activity (Rajan & Muraleedharan, ). In other reports concerning different gallic acid glycosides, it was shown that propyl gallate inhibited the expression of iNOS in rats (Zheng et al, ), and methyl gallate inhibited NO production and iNOS expression (Chae et al, ; Correa et al, ). In addition, it has been proposed that gallic acid has anticancerogenic activity through different mechanisms (Subramanian et al, ).…”

Section: Resultsmentioning

confidence: 87%

Characterization ofVicia(Fabaceae) seed water extracts with potential immunomodulatory and cell antiproliferative activities

et al. 2018

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Vicia seeds represent an underutilized source of nutritional and functional components. Besides being rich in proteins, they are also rich in polyphenols, free amino acids, and lectins. These components are enriched in a by‐product of the production of protein isolates, a pH 4 soluble extract. Extracts from six Vicia have been analyzed, and NO radical scavenging and antiproliferative activities have been determined. Canavanine was the main free amino acid, while gallic acid derivatives were the most abundant of polyphenols. Fractionation of the extracts by ultrafiltration rendered a lectin‐free fraction that exhibited nitric oxide radical scavenging activity. Based on this activity, and in the presence of high levels of polyphenols and canavanine that are inhibitors of inducible nitric oxide synthase, it is proposed that these extracts may have an immunomodulatory effect. In addition, some extracts inhibited proliferation of cancerous Caco‐2 cells, further supporting the potential of Vicia extracts as functional components. Practical applications During the production of Vicia protein isolates an aqueous soluble fraction, considered a by‐product, is generated which is rich in polar polyphenols, free amino acids, and water soluble proteins that are of interest from a functional point of view. In six Vicia species this aqueous fraction showed NO radical scavenging and cell antiproliferative activity. Hence, these fractions usually considered as a by‐product, could be exploited as a source of functional compounds with potential health promoting properties. This could result in the revalorization of Vicia seeds and decrease in the generation of by‐products from the process of Vicia protein isolate productions.

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Methyl Gallate Inhibits the Production of Interleukin-6 and Nitric Oxide via Down-Regulation of Extracellular-Signal Regulated Protein Kinase in RAW 264.7 Cells

Cited by 33 publications

References 21 publications

Involvement of heme oxygenase-1 induction in inhibitory effect of ethyl gallate isolated from Galla Rhois on nitric oxide production in RAW 264.7 macrophages

Involvement of heme oxygenase-1 induction in inhibitory effect of ethyl gallate isolated from Galla Rhois on nitric oxide production in RAW 264.7 macrophages

Methyl Gallate Inhibits Osteoclast Formation and Function by Suppressing Akt and Btk-PLCγ2-Ca2+ Signaling and Prevents Lipopolysaccharide-Induced Bone Loss

Characterization ofVicia(Fabaceae) seed water extracts with potential immunomodulatory and cell antiproliferative activities

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