Methoxyeugenol regulates the p53/p21 pathway and suppresses human endometrial cancer cell proliferation

Costa, Bruna Pasqualotto; Nassr, Marcella Tornquist; Diz, Fernando Mendonça; Antunes, Krist Helen; Antunes, Géssica Luana; Grün, Lucas Kich; Barbé-Tuana, Florencia Marı́a; Nunes, Fernanda Bordignon; Branchini, Gisele; Oliveira, Jarbas Rodrigues de

doi:10.1016/j.jep.2020.113645

Cited by 13 publications

(9 citation statements)

References 79 publications

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“…It was found that the protein expression level of p-Cdc42 was markedly decreased following the addition of Que; however, its expression after 4 h of Que treatment was slightly increased compared with that after 2 h. This may be caused by the negative feedback regulation mechanism, which requires further investigation. p21 was first discovered as a downstream gene of p53, and subsequently, scientists have conducted numerous studies examining p21 and have shown that it is an important cell cycle government target (52,53). p21 is involved in the process of cell proliferation, differentiation, senescence and death (54).…”

Section: Discussionmentioning

confidence: 99%

EGFR and ERK activation resists flavonoid quercetin‑induced anticancer activities in human cervical cancer cells in vitro

Chen

Zhang

et al. 2021

Oncol Lett

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In the present study, due to the complex and numerous targets of Sarcandrae Herb (also known as Zhong Jie Feng), network pharmacology was performed to analyze its therapeutic effect on 2 cervical cancer cell lines, which could assist with the development of novel therapies. The results suggested that the natural flavonoid quercetin (Que), the effective antitumor ingredient in SH, which is widely present in a variety of plants, may depend on the target, EGFR. Previous studies have shown that EGFR serves a crucial role in the occurrence and development of cervical cancer, but its downstream molecules and regulatory mechanisms remain unknown. The anti-cervical cancer cell properties of Que, which are present in ubiquitous plants, were examined in vitro to identify the association between Que and its underlying pathway using MTT assays, flow cytometry, western blot analysis and Transwell assays. It was found that Que reduced cervical cancer cell viability, promoted G 2 /M phase cell cycle arrest and cell apoptosis, as well as inhibited cell migration and invasion. The Tyr1068 phosphorylation site of EGFR and the corresponding ERK target were also examined and the 2 kinases were markedly activated by Que. Furthermore, the EGFR inhibitor, afatinib and the ERK inhibitor, U0126 blocked the increase of EGFR and ERK phosphorylation, and resulted in a notable enhancement of apoptosis and cell cycle arrest. Therefore, to the best of our knowledge, the current results provided the first evidence that EGFR and ERK activation induced by Que could resist Que-induced anticancer activities. On this basis, the present study determined the role of EGFR and the underlying signaling pathways involved in the anti-cervical cancer malignant behavior induced by Que and identified the negative regulatory association.

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Section: Discussionmentioning

confidence: 99%

EGFR and ERK activation resists flavonoid quercetin‑induced anticancer activities in human cervical cancer cells in vitro

Chen

Zhang

et al. 2021

Oncol Lett

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“…In a previous work by our research group, methoxyeugenol showed an effect in decreasing cell proliferation in human endometrial cancer (Costa et al, 2021). HSCs are responsible for extracellular matrix deposition and synthesis, and their activation and proliferation rate are closely linked to the development of fibrosis.…”

Section: Introductionmentioning

confidence: 79%

Methoxyeugenol deactivates hepatic stellate cells and attenuates liver fibrosis and inflammation through a PPAR-ɣ and NF-kB mechanism

Basso

Haute

Ortega‐Ribera

et al. 2021

Journal of Ethnopharmacology

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“…Many natural phytochemical products can suppress cancerous cell proliferation by halting cell cycle phases [21,23]. In the G0/G1 phase, cells are stopped at the G1 to S checkpoint transition by suppressing the function of the complex formed by combining cyclin E and cyclin dependent kinase-2, which are the main cell cycle regulators, to promote cell transition from the G1 phase to the S control point [24].…”

Section: Discussionmentioning

confidence: 99%

Investigation of the effect of yarrow (Achillea millefolium) on Ehrlich ascites tumor

Nisari

İnanç

Tokpınar

et al. 2022

Postępy Higieny I Medycyny Doświadczalnej

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Introduction One of the most important health problems today is cancer. The aim of this study was to investigate the in vitro effect of yarrow (Y) with known anticarcinogenic effect on Ehrlich ascites tumor (EAT). Materials and Methods The above-ground part (300 g) of Y was macerated with water and extracted three times for 24 hours at 37°C in a shaking water bath. In the study, EAT cells were divided into control, DMSO group 5-FU, 50, 100, 200, 400 and 800 μg/ml YP groups. Results At the end of the hour, it was observed that total apoptosis increased significantly in Y groups (especially 50 μg/ml) compared to the control group (p<0.05). It was observed that Y slowed the division of EAT cells (especially 800 μg/ml) by stopping the cell cycle at the G0/G1 stage. It was concluded that Y (especially at high doses) triggered apoptosis by significantly increasing the percentage of total depolarized cells (p<0.001) in all three time periods. Conclusions The results obtained showed that Y extract may have an antitumoral effect on EAT cells. It is thought that this study will contribute to studies on cancer treatment.

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Methoxyeugenol regulates the p53/p21 pathway and suppresses human endometrial cancer cell proliferation

Cited by 13 publications

References 79 publications

EGFR and ERK activation resists flavonoid quercetin‑induced anticancer activities in human cervical cancer cells in vitro

EGFR and ERK activation resists flavonoid quercetin‑induced anticancer activities in human cervical cancer cells in vitro

Methoxyeugenol deactivates hepatic stellate cells and attenuates liver fibrosis and inflammation through a PPAR-ɣ and NF-kB mechanism

Investigation of the effect of yarrow (Achillea millefolium) on Ehrlich ascites tumor

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