Methoxy VO–salen Stimulates Pancreatic β Cell Survival by Upregulation of eNOS and Downregulation of Apoptosis in STZ-induced Diabetic Rats

Abstract

The present study was designed to investigate the effect of MetVO-salen in ameliorating diabetes and oxidative stress in the pancreas of diabetic rats. Streptozotocin (STZ)-induced diabetic rats were treated with MetVO-salen complex intraperitonially (0.3 and 0.6 mg/kg) thrice a week and continued for 8 weeks. Total cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides in serum, and blood glucose were estimated. Furthermore, oxidative stress in rats was also investigated in terms of superoxide dismutase (SOD), catalase, lipid peroxidation, and glutathione (GSH). In addition, the anti-diabetic activity of MetVO-salen was also investigated by assessing histopathological, immunohistochemical in terms of endothelial nitric oxide synthase expression, and apoptotic events in pancreas. Treatment with MetVO-salen complex reduced the blood glucose level and significantly altered the serum biochemical parameters of diabetic rats. Treatment with above complex decreased the lipid peroxidation and the antioxidant enzymes such as SOD, CAT, and GSH to near-control levels. Histopathological, immunohistochemical, and apoptotic studies also revealed that MetVO-salen-induced amelioration of the diabetic state appears to be significant to the preservation of a functional portion of the pancreatic β cells which initially prevent STZ toxicity. This study provides new direction for the management of diabetes but needs further clinical evaluation.

Naringenin attenuates testicular damage, germ cell death and oxidative stress in streptozotocin induced diabetic rats: naringenin prevents diabetic rat testicular damage

“…Oxidative stress depicts the existence of products called free radicals and reactive oxygen species (ROS) formed under normal physiological conditions but become deleterious when not being quenched by the antioxidant systems (Fang et al 2002). When the balance is disrupted towards an over abundance of ROS, oxidative stress (OS) occurs in STZ induced diabetic rats (Roy et al 2011). Oxidative stress plays a role in the development of diabetic complications (Sexton and Jarow 1997).…”

“…It is one of the most commonly used substances to induce diabetes in experimental animals (Szkudelski 2001). Streptozotocin causes alkylation or breakage of DNA strands and a consequent increase in the activity of poly-ADP-ribose synthetase, an enzyme depleting the NAD + in β cells, leading to energy deprivation and finally apoptosis of the β cells of the pancreas (Roy et al 2011) and in the diabetic rat testis (Guneli et al 2008).…”

A novel role for carbon monoxide as a potent regulator of intracellular Ca2+ and nitric oxide in rat pancreatic acinar cells

“…conducted as reported previously (48). In brief, paraffin sections (3 µM) from the pancreatic tissues originally fixed in buffered formalin were deparaffinized and rehydrated, and heated in citrate buffer (pH 6.0) for 20 min.…”

Assemblies of salen-type oxidovanadium(iv) complexes: substituent effects and in vitro protein tyrosine phosphatase inhibition

“…16 Salen type oxidovanadium complexes were found to exhibit insulin-mimetic and proteintyrosine phosphatase (PTP) inhibiting behavior. [17][18][19][20][21][22] Interestingly, oxidovanadium(IV) salen-type complexes were found to exist in either monomeric or polymeric forms in the solid state. 23,24 Oxidovanadium(IV) species with structures consisting of infinite polymeric chains bonded through V-O-V bridges are orange, brown or even black while the monomeric square pyramidal oxidovanadium(IV) species are usually green.…”