Methotrexate Loaded Self Stabilized Calcium Phosphate Nanoparticles: A Novel Inorganic Carrier for Intracellular Drug Delivery

Ukawala, Mukesh; Kulkarni, Vijay; Tushar, R Patel; Murthy, R. S. R.

doi:10.1166/jbn.2009.026

Cited by 37 publications

(22 citation statements)

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“…401 Methotrexate, another anticancer drug, was also successfully loaded into 250-nm sized CAP particles prepared by reverse micelle technique. 402 CAP nanoparticles conjugated with cis-diamminedichloro-platinum (CDDP, cisplatin) were also prepared, exhibiting twice higher IC 50 values when compared to the free drug in an in vitro cell proliferation assay using a human ovarian cancer cell line. 403 Folic acid with its high affinity for folate receptors that are often overexpressed in cancer cells presents another compound that may be added to CAP nanoparticle carriers.…”

Section: Prospective Applicationsmentioning

confidence: 99%

Nanosized hydroxyapatite and other calcium phosphates: Chemistry of formation and application as drug and gene delivery agents

2010

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The first part of this review looks at the fundamental properties of hydroxyapatite (HAP), the basic mineral constituent of mammalian hard tissues, including the physicochemical features that govern its formation by precipitation. A special emphasis is placed on the analysis of qualities of different methods of synthesis and of the phase transformations intrinsic to the formation of HAP following precipitation from aqueous solutions. This serves as an introduction to the second part and the main subject of this review, which relates to the discourse regarding the prospects of fabrication of ultrafine, nanosized particles based on calcium phosphate carriers with various therapeutic and/or diagnostic agents coated on and/or encapsulated within the particles. It is said that the particles could be either surface-functionalized with amphiphiles, peptides, proteins, or nucleic acids or injected with therapeutic agents, magnetic ions, or fluorescent molecules. Depending on the additive, they could be subsequently used for a variety of applications, including the controlled delivery and release of therapeutic agents (extracellularly or intracellularly), magnetic resonance imaging and hyperthermia therapy, cell separation, blood detoxification, peptide or oligonucleotide chromatography and ultrasensitive detection of biomolecules, and in vivo and in vitro gene transfection. Calcium phosphate nanoparticles as carriers of therapeutic agents that would enable a controlled drug release to treat a given bone infection and at the same be resorbed in the body so as to regenerate hard tissue lost to disease are emphasized hereby as one of the potentially attractive smart materials for the modern medicine.

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Section: Prospective Applicationsmentioning

confidence: 99%

Nanosized hydroxyapatite and other calcium phosphates: Chemistry of formation and application as drug and gene delivery agents

2010

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“…Selected examples and typical applications of each category of nanodimensional materials and their use in biomedical applications are available in literature [116]. It is important to note, that in literature on calcium orthophosphates there are cases, when the prefix "nano" has been applied for the structures, with the minimum dimensions exceeding 100 nm[44, 83,[117][118][119][120][121][122][123][124][125][126][127][128][129][130][131][132][133].…”

Section: General Information On "Nano"mentioning

confidence: 99%

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Dorozhkin¹

2012

AJBE

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Nano-sized particles and crystals play an important role in the formation of calcified tissues of various animals. For example, nano-sized and nanocrystalline calcium orthophosphates in the form of apatites of biological origin represent the basic inorganic building blocks of bones and teeth of mammals. Namely, according the recent developments in biomineralization, tens to hundreds nanodimensional crystals of a biological apatite are self-assembled into these complex structures. This process occurs under a strict control by bioorganic matrixes. Furthermore, both a greater viability and a better proliferation of various types of cells have been detected on smaller crystals of calcium orthophosphates. Thus, the nano-sized and nanocrystalline forms of calcium orthophosphates have a great potential to revolutionize the hard tissue-engineering field, starting from bone repair and augmentation to controlled drug delivery systems. This review reports on current state of the art and recent developments on the subject, starting from synthesis and characterization to biomedical and clinical applications. Furthermore, the review also discusses possible directions for future research and development.

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“…ACCEPTED MANUSCRIPT 4 reasons behind choosing CPNP -i) tetracycline has higher affinity of chelation with divalent metal ions [16,17] and so, it can easily bind with CPNP, ii) CPNP is used as a potential carrier of DNA [18,19,20,21,22], RNA [23,24], proteins [25,26] and various therapeutic drugs [27,28,29,30,31] into different cells and iii) calcium phosphate, being a principal component of human bones and teeth, is highly biocompatible and biodegradable [32,33]. Here, we report that the Tet-CPNP killed different multi-drug resistant diarrhea-causing bacteria viz., Escherichia coli, Salmonella kentuckey and Shigella flexneri in vitro; in vivo killing of Shigella flexneri that infected Zebra fish larvae was also observed.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Tetracycline-loaded calcium phosphate nanoparticle (Tet-CPNP): Rejuvenation of an obsolete antibiotic to further action

Mukherjee

Patra

Dutta

et al. 2016

Biochimica et Biophysica Acta (BBA) - General Subjects

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Methotrexate Loaded Self Stabilized Calcium Phosphate Nanoparticles: A Novel Inorganic Carrier for Intracellular Drug Delivery

Cited by 37 publications

References 0 publications

Nanosized hydroxyapatite and other calcium phosphates: Chemistry of formation and application as drug and gene delivery agents

Nanosized hydroxyapatite and other calcium phosphates: Chemistry of formation and application as drug and gene delivery agents

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Tetracycline-loaded calcium phosphate nanoparticle (Tet-CPNP): Rejuvenation of an obsolete antibiotic to further action

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