Methotrexate-induced toxic effects and the ameliorating effects of astaxanthin on genitourinary tissues in a female rat model

Günyeli, İlker; Saygın, Mustafa; Özmen, Özlem

doi:10.1007/s00404-021-06000-2

Cited by 8 publications

(7 citation statements)

References 34 publications

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“…Although many beneficial biological properties of ASX have been demonstrated in experimental models [8,33], investigation of its ovoprotective effect has become popular in recent years [28]. Our histological findings were consistent with the results of previous studies demonstrating the ovarian protective effect of ASX [11,34,35].…”

Section: Discussionsupporting

confidence: 91%

“…It is thought that ASX's ROS scavenging activity and its potential to bind to DNA play a central role in the elimination of OS and DNA damage exacerbated by 5-FU. Consistently, it was previously reported that ASX could exert tissue protective activity by modulating OS, DNA damage and the capacity of antioxidant system in various experimental models [1,11,12,[14][15][16].…”

Section: Discussionsupporting

confidence: 80%

“…The cooperation of OS and inflammation activates apoptosis, causing permanent tissue damage and tissue death [1]. Since there is no approved treatment protocol or a specific antidote to be used against the toxic effects of chemotherapeutic use, different toxicity prevention strategies are currently being studied meticulously [10,11]. In this context, the therapeutic effects of molecules with antioxidant and antiinflammatory properties against chemotherapeutic-induced tissue damage are frequently investigated [1,3,5].…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic Effect of Astaxanthin on 5-Fluorouracil-Induced Ovarian Damage in Rats

Ayazoglu Demir,

Mentese,

Livaoglu

et al. 2024

Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi

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Although astaxanthin (ASX) is one of the most studied antioxidant molecules, its curative effect against ovarian damage caused by 5-fluorouracil (5-FU) has not been demonstrated to date. It was therefore aimed to investigate whether ASX is therapeutic against 5-FU-induced ovotoxicity in this study. Rats were first exposed to 5-FU (100 mg/kg) and then treated ASX (250 μg/kg) for three days. Oxidative stress (OS), inflammation and apoptosis markers were determined using spectrophotometric methods. Ovarian tissues were also evaluated histologically. The levels of OS, inflammation and apoptosis biomarkers increased by 5-FU administration (p<0.05). Treatment with ASX significantly alleviated these markers (p<0.05). These findings reveal that ASX may exert an ovoprotective effect by reducing pro-inflammatory mediators and enhancing antioxidant status in ovarian tissue.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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Therapeutic Effect of Astaxanthin on 5-Fluorouracil-Induced Ovarian Damage in Rats

Ayazoglu Demir,

Mentese,

Livaoglu

et al. 2024

Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi

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“…TOS and OSI levels increased significantly with MTX application in ovarian tissue, and these values were shown to decrease with AXA application. At the same time, Gunyeli et al (41) reported that TAS levels decreased with MTX and increased with AXA administration. Our findings supported previous reports that MTX administration induces oxidative stress in various tissues (42)(43)(44).…”

Section: Discussionmentioning

confidence: 91%

“…Compared to groups 2 and 3, OSI levels were significantly reduced with RES administration in group 5 and no significant increase in TAS levels was noted. Gunyeli et al (41), in their study with Astaxanthin (AXA) against MTX induced ovarian damege, analyzed TOS, OSI, and TAS levels. TOS and OSI levels increased significantly with MTX application in ovarian tissue, and these values were shown to decrease with AXA application.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Siçanlarda Oksi̇dati̇f Stresi̇ Ve Apoptozu Baskilayarak Metotreksat Kaynakli Yumurtalik Hasarini Hafi̇fleti̇r

AKA

Acar

Özgöçmen

et al. 2022

SDÜ Tıp Fakültesi Dergisi

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Abstract Objective: The study aimed to evaluate the protective effects of resveratrol (RES) against methotrexate (MTX)-induced ovarian damage in female rats and were divided into 7 groups: Material and Methods: Control; MTX (15mg/kg-1st day); MTX (1st and 3rd day); MTX (1st, 3rd and 5th day); MTX (1st day) + RES (20mg/kg); MTX (1st and 3rd day) + RES; MTX (1st, 3rd and 5th day) + RES. Since there was only one animal left in groups 4 and 6 and no animals in group 7, these groups were excluded from the evaluation. Ovarian damage was evaluated by performing hematoxylin-eosin (H-E) and TUNEL staining on ovarian tissues of rats. TAS and TOS activities in ovarian tissues were evaluated spectrophotometrically. In addition, DNA damage in blood was evaluated by comet assay. Results: As a result of biochemical analyzes, TOS levels increased in MTX groups, group 2 and 3, compared to the control group, while TOS level decreased significantly in group 5. On the other hand, TAS level increased significantly in group 5 compared to groups 2 and 3. H-E staining results showed that MTX-induced damage improved with RES (group 5) treatment. Maximum positive staining in TUNEL image; observed in equal amounts in groups 2 and 3, then in group 5. In addition, the comet score, which indicates DNA damage, increased significantly in groups 2 and 3, while it decreased significantly in group 5. Conclusion: In line with the results of this study, it was observed that resveratrol may provide protective effects against methotrexate-induced ovarian damage due to its antioxidant properties.

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