Methotrexate combined with methylprednisolone for the recovery of motor function and differential gene expression in rats with spinal cord injury

Liu, Jiantao; Zhang, Si; Gu, Bing; Li, Huanan; Wang, Shuo-Yu; Shui-yin, Zhang

doi:10.4103/1673-5374.215263

Cited by 12 publications

(3 citation statements)

References 67 publications

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“…TGF-β has a complex character in tissue repair after SCI. previous studies have shown that pathological condition in the brain and spinal cord can alter the level of cytokine/ growth factor such as TGF-β that is considered a pleiotropic cytokine (20,21). Some studies suggest that TGF-β has a key role in modulating infl ammatory responses in SCI.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effect of BMSCs condition media and methylprednisolone in TGF-β expression and functional recovery after an acute spinal cord injury

Khoshsirat¹,

Abbaszadeh²,

Ahrabi³

et al. 2018

BLL

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The purpose of this experimental study is evaluation of the effect of BMSCs Condition Media and Methylprednisolone in TGF-β expression and functional recovery after acute spinal cord injury in adult wistar rat. MATERIAL AND METHODS: After an acute spinal cord injury, MP and BMSC-CM were injected intraperitoneally and the recovery rate was evaluated by BBB and narrow beam test. Real time PCR, H&E staining, cavity formation and stereology was done after 12 weeks post injury. RESULT: There were signifi cant differences in functional recovery between MP+CM group, relative to other groups. TGF-β1 expression decreased in MP+CM group compared to the control group. Cavity size in experimental groups decreased compared to the control group. The mean volume of spinal cord demonstrated a signifi cant increase in the MP+CM group in comparison to the other experimental groups. CONCLUSION: Simultaneous use of MP and BMSC-CM will improve recovery from the spinal cord injury, reduce infl ammation and improve functional recovery (Tab. 1, Fig. 8, Ref. 26).

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Section: Discussionmentioning

confidence: 99%

Evaluation of the effect of BMSCs condition media and methylprednisolone in TGF-β expression and functional recovery after an acute spinal cord injury

Khoshsirat¹,

Abbaszadeh²,

Ahrabi³

et al. 2018

BLL

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“…Eighteen adult female SD rats weighing 270 to 300 g were randomly divided into the model group, BMSC group, and FAS + BMSC group. Each animal was subjected to a moderate contusion model of SCI as previously described, with minor modification [22]. A dorsal laminectomy was performed at the T9-T10 levels under sodium pentobarbital (40 mg/kg, i.p.)…”

Section: Methodsmentioning

confidence: 99%

Fasudil Promotes BMSC Migration via Activating the MAPK Signaling Pathway and Application in a Model of Spinal Cord Injury

Zhan

Chen

et al. 2018

Stem Cells International

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Bone marrow-derived mesenchymal stem cells (BMSCs) are considered as transplants for the treatment of central nervous system (CNS) trauma, but the therapeutic effect is restricted by their finite mobility and homing capacity. Fasudil (FAS), a potent Rho kinase inhibitor, has been reported to alleviate nerve damage and induce the differentiation of BMSCs into neuron-like cells. However, the effect of FAS on the migration of BMSCs remains largely unknown. The present study revealed that FAS significantly enhanced the migration ability and actin stress fiber formation of BMSCs in vitro with an optimal concentration of 30 μmol/L. Moreover, we found that activation of the MAPK signaling pathway was involved in these FAS-mediated phenomena. In vivo, cells pretreated with FAS showed greater homing capacity from the injection site to the spinal cord injury site. Taken together, the present results indicate that FAS acts as a promoting factor of BMSC migration both in vitro and in vivo, possibly by inducing actin stress fiber formation via the MAPK signaling pathway, suggesting that FAS might possess synergistic effect in stem cell transplantation of CNS trauma.

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“…Methylprednisolone (MP) has the anti-inflammatory activity more than three times than that of prednisolone ( 20 ) and is more effective than dexamethasone in reducing complications associated with hospitalized hypoxic COVID-19 patients and following major hepatectomy ( 21 , 22 ). Moreover, studies have indicated that MP can decrease inflammation and aid in the recovery of neurological function in various conditions, including spinal cord injury ( 23 , 24 ), crush-type sciatic nerve injury ( 25 ), and lung injury induced by brain death ( 26 , 27 ). MP exerts multiple anti-inflammatory effects, such as inhibiting the activation of T cells, dampening the inflammatory cytokine cascade, decreasing immune cells’ extravasation into the central nervous system, and promoting apoptosis of activated immune cells ( 28 ).…”

Section: Introductionmentioning

confidence: 99%

Methylprednisolone alleviates cognitive functions through the regulation of neuroinflammation in Alzheimer’s disease

Sun

et al. 2023

Front. Immunol.

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Alzheimer’s disease (AD) is a progressive neurodegenerative disease and linked to abnormal deposition of amyloid-β (Aβ), neurofibrillary tangles (NFTs), synaptic dysfunction, and neuroinflammation. Despite significant progress in unravelling the pathogenesis of AD, currently main therapeutic interventions is limited to symptomatic alleviation. Methylprednisolone (MP), a synthetic glucocorticoid, is recognized for its extensive anti-inflammatory properties. Our study assessed the neuroprotective effect of MP (25 mg/kg) administration to an Aβ1-42-induced AD mouse model. Our findings demonstrate that MP treatment can ameliorate cognitive impairment in Aβ1-42-induced AD mice and suppress microglial activation in the cortex and hippocampus. RNA-Sequencing analysis reveals that MP ultimately rescues cognitive dysfunction through improving the synapse function and inhibiting the immune and inflammatory processes. Our study suggests that MP could be a promising drug alternative for the treatment of AD, either alone or in combination with other existing drugs.

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Methotrexate combined with methylprednisolone for the recovery of motor function and differential gene expression in rats with spinal cord injury

Cited by 12 publications

References 67 publications

Evaluation of the effect of BMSCs condition media and methylprednisolone in TGF-β expression and functional recovery after an acute spinal cord injury

Evaluation of the effect of BMSCs condition media and methylprednisolone in TGF-β expression and functional recovery after an acute spinal cord injury

Fasudil Promotes BMSC Migration via Activating the MAPK Signaling Pathway and Application in a Model of Spinal Cord Injury

Methylprednisolone alleviates cognitive functions through the regulation of neuroinflammation in Alzheimer’s disease

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