“…Different methods based on clinical evaluation [3] and biological tests have been developed to diagnose PROM, among which the detection of proteins such as prolactin [4], a-fetoprotein [5], diamine oxidase [6,7], insulin-like growth factor binding protein-1 (IGFBP-1) [7,8], human choriogonadotrophin [9], fetal fibronectin [10,11], cytokines [12], interleukin-6 [12], interleukin-18 [13], as well as decidual relaxins H1 and H2 proteins [14], usually determined by immunoassays. One of the problems encoun-tered with these proteins is that even if they are present in amniotic fluid (AF) at concentrations higher than those in the maternal plasma, the contamination of vaginal section sample by maternal blood can lead to false-positive results.…”