Methods used for the killing and preservation of blowfly larvae, and their effect on post-mortem larval length

Adams, Z. J. O.; Hall, M. J. R.

doi:10.1016/j.forsciint.2003.08.010

Cited by 159 publications

(145 citation statements)

References 11 publications

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“…One such factor is the method of killing and preservation of the maggots collected from a corpse. Adams and Hall (2003), for example, found that preservatives containing high concentrations of ethanol, such as the KAA Þxative used in our study, can cause signiÞcant postmortem expansion in larval length. Fortunately, all of the specimens collected from ethanoltreated and untreated pigs in our study were treated similarly, and, in addition, our objective was not to estimate PMI but to show that carcass tissue containing substances such as alcohol can alter the development of carrion-feeding insects.…”

Section: Discussionmentioning

confidence: 51%

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Effects of Antemortem Ingestion of Ethanol on Insect Successional Patterns and Development ofPhormia regina(Diptera: Calliphoridae)

et al. 2005

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The effects of antemortem ingestion of ethanol by domestic pigs, Sus scrofa L., on postmortem insect successional patterns and the development of Phormia regina (Meigen) were studied during summer 2003 in Blacksburg, VA. Insect samples were collected from the carcasses of ethanol-treated and untreated pigs for 10 d postmortem during two successional studies. In total, 32 insect taxa were collected during the two studies, with 29 and 27 taxa observed on the carcasses of ethanol-treated and untreated pigs, respectively. The earliest arrivers to both carcass types were dipterans. This group was represented by six families, with P. regina and Phaenicia coeruleiviridis (Macquart) being the most common calliphorids. Beetles in six families were collected on the carcasses of ethanol-treated pigs, but only three of the families were collected on carcasses of the untreated pigs. Permutation analyses to test the null hypothesis of no similarity between successional patterns of insect taxa from carcasses of ethanol-treated and untreated pigs showed that the successional patterns were similar between carcass types in the Þrst (P ϭ 0.003) and the second (P ϭ 0.01) studies. The results of the development study of P. regina maggots in the Þeld show that there was a signiÞcant difference between the distributions of length for maggots reared on loin tissue from ethanol-treated and untreated pigs. Maggots that fed on tissue from ethanol-treated pigs took Ϸ11.9 h longer to reach the pupal stage than maggots that fed on tissue from untreated pigs. The longer developmental time for maggots on tissue from ethanol-treated pigs was due mainly to the longer postfeeding period of the third instar.

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Section: Discussionmentioning

confidence: 51%

“…Factors that affect postmortem larval length, a measure of larval age, also can affect the accuracy of development-based PMI estimates (Adams and Hall 2003). One such factor is the method of killing and preservation of the maggots collected from a corpse.…”

Section: Discussionmentioning

confidence: 99%

Effects of Antemortem Ingestion of Ethanol on Insect Successional Patterns and Development ofPhormia regina(Diptera: Calliphoridae)

et al. 2005

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“…One larva was sampled from ten random cups every 3h for the first 48h and, thereafter, every 6h until they pupated. Each larva sampled was immediately placed in boiling water for 30s and then transferred into 70% ethanol for 1-2h, which killed them but did not allow them to contract or swell [23].…”

Section: Protocolmentioning

confidence: 99%

Effect of temperature on development of the blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae)

2015

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The blowfly Lucilia cuprina is a primary colonizer of decaying vertebrate carrion, and its development provides a temperature-dependent clock that may be used to estimate the post-mortem interval of corpses and carcasses in medicolegal forensic investigations. This study uses the development of L. cuprina raised on a substrate of chicken liver at six constant temperatures from 18 to 33 °C to calibrate a thermal accumulation model of development for forensic applications. Development was optimal near 24 °C; above this temperature, survival of post-feeding life stages was increasingly compromised, while below it, development was increasingly retarded.The lower developmental threshold (~12 °C) and thermal summation constants of L. cuprina are distinct from those reported for Lucilia sericata, verifying that it is essential to identify African Lucilia specimens accurately when using them to estimate post-mortem intervals.

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“…The same methods of collection and preservation described by Lord and Burger (1983) [5], Adams and Hall (2003) [6] and Amendt et al (2006) [7]. Collection kits with an illustrated guide are prepared by the laboratory of Entomology of the NICC and provided to investigators all over the country.…”

Section: Samplingmentioning

confidence: 99%

Identification and Occurrence Records of Lucilia Sericata Meigen (Diptera, Calliphoridae) In Algeria

Taleb¹,

Tail²,

Kara³

et al. 2015

International Conference on Chemical, Agricultural and Biological Sciences (CABS-2015) Sept. 4-5, 2015 Istanbul (Turkey)

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Methods used for the killing and preservation of blowfly larvae, and their effect on post-mortem larval length

Cited by 159 publications

References 11 publications

Effects of Antemortem Ingestion of Ethanol on Insect Successional Patterns and Development ofPhormia regina(Diptera: Calliphoridae)

Effects of Antemortem Ingestion of Ethanol on Insect Successional Patterns and Development ofPhormia regina(Diptera: Calliphoridae)

Effect of temperature on development of the blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae)

Identification and Occurrence Records of Lucilia Sericata Meigen (Diptera, Calliphoridae) In Algeria

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