Methods toward in vivo measurement of zebrafish epithelial and deep cell proliferation

Campana, Matteo; Maury, Benoit; Dutreix, Marie; Peyriéras, Nadine; Sarti, Alessandro

doi:10.1016/j.cmpb.2009.08.008

Cited by 8 publications

(9 citation statements)

References 32 publications

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“…These observations are consistent with the sensitivity to antitumoral activity of zebrafish embryonic cells that share characteristic properties with tumor cells, including mitotic index and, biochemical and phenotypic traits. 15, 24 Consistent with this hypothesis, we recently developed a method for measuring embryo cell proliferation 25 and observed Dbait anti-proliferation activity by direct intracellular injection of Dbait into zebrafish blastomeres (unpublished data).…”

Section: Discussionmentioning

confidence: 70%

Comparison of distribution and activity of nanoparticles with short interfering DNA (Dbait) in various living systems

et al. 2011

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Introducing small DNA molecules (Dbait) impairs the repair of damaged chromosomes and provides a new method for enhancing the efficiency of radiotherapy in radio-resistant tumors. The radiosensitizing activity is dependent upon the efficient delivery of Dbait molecules into the tumor cells. Different strategies have been compared, to improve this key step. We developed a pipeline of assays to select the most efficient nanoparticles and administration protocols before preclinical assays: (i) molecular analyses of complexes formed with Dbait molecules, (ii) cellular tests for Dbait uptake and activity, (iii) live zebrafish embryo confocal microscopy monitoring for in vivo distribution and biological activity of the nanoparticles and (iv) tumor growth and survival measurement on mice with xenografted tumors. Two classes of nanoparticles were compared, polycationic polymers with linear or branched polyethylenimine (PEI) and covalently attached cholesterol (coDbait). The most efficient Dbait transfection was observed with linear PEI complexes, in vitro and in vivo. Doses of coDbait ten-fold higher than PEI/Dbait nanoparticles, and pretreatment with chloroquine, were required to obtain the same antitumoral effect on xenografted melanoma. However, with a 22-fold lower ‘efficacy dose/toxicity dose' ratio as compared with Dbait/PEI, coDbait was selected for clinical trials.

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Section: Discussionmentioning

confidence: 70%

Comparison of distribution and activity of nanoparticles with short interfering DNA (Dbait) in various living systems

et al. 2011

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“…, N θ . In [3,22,18,4,13] the centers detected by LSCD were used for various purposes including cell shape, epithelium and whole embryo segmentation and tracking. …”

Section: Cell Center Detectionmentioning

confidence: 99%

Nonlinear PDE based numerical methods for cell tracking in zebrafish embryogenesis

Mikula

Špir

Smíšek

et al. 2015

Applied Numerical Mathematics

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“…Within the workflow, this step aims at automatically identify and quantify from large imaging datasets morphogenetic events occurring during development, such as cell trajectories or cell shape changes; rate or pattern of cell divisions; cell depth and growth; cell intercalations or tissue invagination. In cell biology, many different features have been computed from imaging data (Carpenter et al .,2006) and can be used in developmental biology. However, some features, such as cell intercalation and tissue invagination are specific to morphogenetic processes and do not have equivalents in cell biology.…”

Section: Feature Computationmentioning

confidence: 99%

“…High‐throughput imaging strategies have been recently developed in cell biology (Pepperkok and Ellenberg,2006). In addition, many studies report imaging of millions of cells, computing of thousands of features and extracting biologically useful information using data classification within the multidimensional space of these features (see (Carpenter et al .,2006; Held et al .,2010; Loo et al .,2007; Perlman et al .,2004), for instance). The investigation of therapeutic effects of drugs based on large scale screens using automated imaging and analysis presented in (Loo et al .,2007) is a striking illustration.…”

Section: Introductionmentioning

confidence: 99%

Toward high‐content/high‐throughput imaging and analysis of embryonic morphogenesis

Truong

Supatto

2011

Genesis

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Summary: In vivo study of embryonic morphogenesis tremendously benefits from recent advances in live microscopy and computational analyses. Quantitative and automated investigation of morphogenetic processes opens the field to high-content and high-throughput strategies. Following experimental workflow currently developed in cell biology, we identify the key challenges for applying such strategies in developmental biology. We review the recent progress in embryo preparation and manipulation, live imaging, data registration, image segmentation, feature computation, and data mining dedicated to the study of embryonic morphogenesis. We discuss a selection of pioneering studies that tackled the current methodological bottlenecks and illustrated the investigation of morphogenetic processes in vivo using quantitative and automated imaging and analysis of hundreds or thousands of cells simultaneously, paving the way for high-content/high-throughput strategies and systems analysis of embryonic morphogenesis. genesis 49:555-569, 2011. V V C 2011 Wiley-Liss, Inc.

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Methods toward in vivo measurement of zebrafish epithelial and deep cell proliferation

Cited by 8 publications

References 32 publications

Comparison of distribution and activity of nanoparticles with short interfering DNA (Dbait) in various living systems

Comparison of distribution and activity of nanoparticles with short interfering DNA (Dbait) in various living systems

Nonlinear PDE based numerical methods for cell tracking in zebrafish embryogenesis

Toward high‐content/high‐throughput imaging and analysis of embryonic morphogenesis

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