Methods to Test Endocrine Disruption in &lt;em&gt;Drosophila melanogaster&lt;/em&gt;

Bovier, Tiziana Francesca; Cavaliere, Daniela; Colombo, Michele; Peluso, Gianfranco; Giordano, Elena; Digilio, Filomena Anna

doi:10.3791/59535

Cited by 7 publications

(5 citation statements)

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“…Animal models, including fruit flies, offer significant potential for studying the physiological and pathological components of diseases or treatments, particularly in the context of antioxidants [ 14 , 15 ]. The life cycle of D. melanogaster , starting from egg to adult, spans approximately 10 days [ 16 ]. Due to their short life cycle, fruit flies can serve as a useful tool for toxicity assessment and evaluating the effectiveness of potential medications in experimental survival studies [ 17 , 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…Fruit fly ( Drosophila melanogaster ) has been proposed as useful animal model for studying the physiological and pathological components of diseases or treatments, including those related to antioxidants [ 14 , 15 ]. With a short lifespan of approximately 10 days from egg to adult, D. melanogaster is an ideal organism for toxicity testing and pharmacological efficacy assessments [ 16 ]. The brief life cycle of D. melanogaster makes them excellent subjects for evaluating toxicity parameters or assessing pharmacological effects [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Phenotypical and molecular assessments on the pharmacological effects of curcumin in Drosophila melanogaster

Rumata¹,

Purwaningsih²,

Asbah³

et al. 2023

Narra J

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Curcumin, a dietary polyphenol derived from turmeric's rhizome, exhibits a range of pharmacological activities, such as antioxidant, analgesic, antipyretic, and anti-inflammatory effects. It has been investigated for its therapeutic potential in chronic obstructive pulmonary disease (COPD) due to its ability to inhibit nuclear factor kappa B (NF-κB), modulate oxidative stress, impact cell viability, and regulate gene expression. However, most studies have been limited to in vitro conditions. To address this gap, we conducted in vivo experiments using the fruit fly Drosophila melanogaster to explore the antioxidant activities and biological significance of curcumin. Several parameters were assessed using different assays, including curcumin toxicity assay, fly survival, locomotor response to curcumin treatment (with or without cigarette smoke), trypan blue staining, larval crawling assays, and gene expression analysis. Our data revealed no significant differences in toxicity and locomotor tests across various curcumin concentrations. D. melanogaster tolerated curcumin at concentrations of 0.5 µM, 5 µM, 50 µM, and 500 µM, suggesting its safety without negatively impacting locomotor. Furthermore, curcumin at 5 µM extended the lifespan of D. melanogasterexposed to cigarette smoke, while reversing the negative effects of smoke exposure on gut cell viability and larval locomotor activity. In conclusion, curcumin administration appeared safe for D. melanogaster, with potential benefits for longevity and locomotory function. These findings support the idea that curcumin possesses in vivo antioxidant properties and may serve as a promising pharmacological agent. However, further study is needed to explore its potential applications in human health and disease management, particularly in the context of COPD.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phenotypical and molecular assessments on the pharmacological effects of curcumin in Drosophila melanogaster

Rumata¹,

Purwaningsih²,

Asbah³

et al. 2023

Narra J

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“…Teglicar (Sigma-Aldrich; Avanti Polar Lipids 870853P; Merk Life Science S.r.l., 20149 Milano, Italy) was added to the surface of the assay fly food (AF, 2% agar, 10% powdered yeast, 10% sucrose, 0.1% Nipagin) at the final concentration of 50 μM, and dried with gentle agitation for 3 h at room temperature [ 64 ]. This food was used for rearing the experimental flies, while the controls were reared in AF without Teglicar.…”

Section: Methodsmentioning

confidence: 99%

The Reversible Carnitine Palmitoyltransferase 1 Inhibitor (Teglicar) Ameliorates the Neurodegenerative Phenotype in a Drosophila Huntington’s Disease Model by Acting on the Expression of Carnitine-Related Genes

et al. 2022

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Huntington’s disease (HD) is a dramatic neurodegenerative disorder caused by the abnormal expansion of a CAG triplet in the huntingtin gene, producing an abnormal protein. As it leads to the death of neurons in the cerebral cortex, the patients primarily present with neurological symptoms, but recently metabolic changes resulting from mitochondrial dysfunction have been identified as novel pathological features. The carnitine shuttle is a complex consisting of three enzymes whose function is to transport the long-chain fatty acids into the mitochondria. Here, its pharmacological modification was used to test the hypothesis that shifting metabolism to lipid oxidation exacerbates the HD symptoms. Behavioural and transcriptional analyses were carried out on HD Drosophila model, to evaluate the involvement of the carnitine cycle in this pathogenesis. Pharmacological inhibition of CPT1, the rate-limiting enzyme of the carnitine cycle, ameliorates the HD symptoms in Drosophila, likely acting on the expression of carnitine-related genes.

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“…Lifespan assay was carried out as previously described [ 74 ]. Briefly, newly emerged adult flies with the desired genotype (P{UASHTT128QFL}f27b/P{w[+mW.hs]=GawB}elav[C155] were collected under cold-induced anesthesia, sorted by sex, grouped into five cohorts of 20 individuals in vials containing 2.0 mL of AF supplemented or not with the different aptamers, and reared at 28 °C.…”

Section: Methodsmentioning

confidence: 99%

Truncated Analogues of a G-Quadruplex-Forming Aptamer Targeting Mutant Huntingtin: Shorter Is Better!

Riccardi

D’Aria

Fasano

et al. 2022

IJMS

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Two analogues of the MS3 aptamer, which was previously shown to have an exquisite capability to selectively bind and modulate the activity of mutant huntingtin (mHTT), have been here designed and evaluated in their physicochemical and biological properties. Featured by a distinctive propensity to form complex G-quadruplex structures, including large multimeric aggregates, the original 36-mer MS3 has been truncated to give a 33-mer (here named MS3-33) and a 17-mer (here named MS3-17). A combined use of different techniques (UV, CD, DSC, gel electrophoresis) allowed a detailed physicochemical characterization of these novel G-quadruplex-forming aptamers, tested in vitro on SH-SY5Y cells and in vivo on a Drosophila Huntington’s disease model, in which these shorter MS3-derived oligonucleotides proved to have improved bioactivity in comparison with the parent aptamer.

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Methods to Test Endocrine Disruption in <em>Drosophila melanogaster</em>

Cited by 7 publications

References 0 publications

Phenotypical and molecular assessments on the pharmacological effects of curcumin in Drosophila melanogaster

Phenotypical and molecular assessments on the pharmacological effects of curcumin in Drosophila melanogaster

The Reversible Carnitine Palmitoyltransferase 1 Inhibitor (Teglicar) Ameliorates the Neurodegenerative Phenotype in a Drosophila Huntington’s Disease Model by Acting on the Expression of Carnitine-Related Genes

Truncated Analogues of a G-Quadruplex-Forming Aptamer Targeting Mutant Huntingtin: Shorter Is Better!

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