Methods to double haploid chromosome numbers

Rao, P. S.; Suprasanna, P.

doi:10.1007/978-94-017-1860-8_18

Cited by 22 publications

(12 citation statements)

References 89 publications

(72 reference statements)

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“…During the normal life cycle of a plant, it is widely accepted that once a cell enters endoreduplication cycles, it irreversibly differentiates and there is no way back to re-enter into a new cell cycle (Joubes and Chevalier, 2000;Larkins et al, 2001). However, under special circumstances, such as in vitro culture conditions, endoreduplicated cells may have the potential to dedifferentiate and re-enter the mitotic cycle (Rao and Suprasanna, 1996). If an endoreduplicated cell re-enters the cell cycle ( Fig.…”

Section: Endoreduplicationmentioning

confidence: 99%

“…It appeared that endoreduplication, alone or combined, is the most likely mechanism to explain ploidy shifts towards triploidy or polyploidy at early stages of microspore androgenic development. Going one step further, endoreduplication was proposed nearly ten years ago as the main mechanism for early chromosome doubling during induction of microspore embryogenesis (Rao and Suprasanna, 1996;Henry, 1998).…”

Section: Endoreduplicationmentioning

confidence: 99%

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Pathways to doubled haploidy: chromosome doubling during androgenesis

Seguí-Simarro

Nuez

2008

Cytogenet Genome Res

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Production of doubled haploid (DH) plants through androgenesis induction is a promising and convenient alternative to conventional selfing techniques for the generation of pure lines for breeding programs. This process comprises two main steps: induction of androgenesis and duplication of the haploid genome. Such duplication is sometimes indirectly induced by the treatments used to promote androgenic development. But usually, an additional step of direct chromosome doubling must be included in the protocol. Duplication of the haploid genome of androgenic individuals has been thought to occur through three mechanisms: endoreduplication, nuclear fusion and c-mitosis. In this review we will revise and analyze the evidences supporting each of the proposed mechanisms and their relevance during androgenesis induction, embryo/callus development and plant regeneration. Special attention will be devoted to nuclear fusion, whose evidences are accumulating in the last years.

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Section: Endoreduplicationmentioning

confidence: 99%

Section: Endoreduplicationmentioning

confidence: 99%

Pathways to doubled haploidy: chromosome doubling during androgenesis

Seguí-Simarro

Nuez

2008

Cytogenet Genome Res

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“…In G. triflora, Doi et al (2010) reported that 105 plants regenerated from anther culture consisted of 4.7 % haploid, 24.8 % diploids, and 70.5 % triploid. A high frequency of triploid and diploid formation is considered to be caused by nuclear fusion, endomitosis, endoreduplication, and unreduced microspores (Keller et al 1987;Rao and Suprasanna 1996). In contrast, of 179 plants regenerated from ovule culture of G. triflora, G. scabra, and their hybrids, 55.9 % were diploid and 31.3 % were haploid, in addition, triploid (5.0 %), tetraploid (6.1 %), hexaploid (0.5 %), and chimeric (1.1 %) plants were also found ).…”

Section: Ploidymentioning

confidence: 99%

Haploid and Doubled Haploid Plant Production in Gentian (Gentiana spp.)

Doi

Takahata

2015

The Gentianaceae - Volume 2: Biotechnology and Applications

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Successful production of haploid and doubled haploid (DH) plants from male and female gametophytic cells by in vitro culture has been established in some gentians (Gentiana triflora, G. scabra, and their hybrid), which are used as ornamental plants. Plant regeneration is obtained by both anther culture (androgenesis) and unfertilized ovule/ovary culture (gynogenesis). Attention is given to culture techniques, factors influencing androgenesis and gynogenesis, the ploidy of regenerants, and utilization of the molecular genetic marker for identification of DH. Although plant regeneration is obtained by both anther culture and unfertilized ovule/ovary culture, the latter has more advantages than the former. In gentian, the establishment of the haploid and DH method has application to F1 breeding and also to basic genetic studies.

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“…Colchicine is known to inhibit mitosis in a wide variety of plant and animal cells by interfering with the structure of the mitotic spindle, but it is highly toxic and often induces chimeric plants (9) and induced embryogenesis. Other antimitotic agents such as trifluralin, oryzalin, pronamide and amiprophosmethyl (APM) have also been tested in sugar beet (8) and their effects were inconsistent in terms of both haploids and doubled haploids.…”

Section: Introductionmentioning

confidence: 99%