Methods to Control the Subcellular Localisation of Fluorescent Sensors

Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life

“…Fluorescent metal ion sensors can be broadly categorized into small molecule indicators and genetically encodable indicators . Recent reviews focused on the former category have highlighted progress in subcellular targeting, , activity based sensing for improving selectivity, two-photon sensing, and application of sensors beyond mammalian systems . Genetically encodable indicators include those based on fluorescent proteins as well as those based on bioluminescent proteins, nucleic acids, and hybrid systems, which combine synthetic with genetically encodable components (chemigenetic sensors). , Here, we focus on fluorescent protein-based sensors, which are the largest and most well-developed class of genetically encoded metal ion sensors.…”