Methods of surgical sperm extraction and implications for assisted reproductive technology success

Miyaoka, Ricardo; Orosz, José Eduardo; Achermann, Arnold Peter Paul; Esteves, Sandro C.

doi:10.23736/s0031-0808.18.03508-5

Cited by 30 publications

(18 citation statements)

References 85 publications

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“…With the evolution of intracytoplasmic sperm injection (ICSI), certain subtypes of azoospermia have become treatable, where spermatozoa are retrieved from testes in the so‐called surgical sperm retrieval techniques (SSR) (Schlegel, 2009; Wosnitzer & Goldstein, 2014). Another breakthrough for azoospermia has been the ability to successfully cryopreserve the retrieved spermatozoa for use in ICSI (Miyaoka, Orosz, Achermann, & Esteves, 2018). However, whether the procedure of cryopreservation for surgically retrieved testicular spermatozoa can induce further stress, such as oxidative stress (OS), remains an open research question.…”

Section: Introductionmentioning

confidence: 99%

Testicular tissue oxidative stress in azoospermic patients: Effect of cryopreservation

et al. 2020

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This study evaluated oxidative stress (OS) in human testicular tissue in obstructive (OA) and nonobstructive azoospermia (NOA) before and after cryopreservation. Azoospermic patients were subjected to surgical sperm retrieval (SSR) and cryopreservation. Assay of catalase activity (CAT) and malondialdehyde level (MDA) using colorimetric methods was done. The number of retrieved spermatozoa and the Johnson score were assessed in fresh testicular samples. The study included 21 OA (group A) and 16 positive sperm retrieval (group B, positive NOA). The basal CAT activity in the positive NOA group was significantly higher than that in the OA group. Basal MDA level was also significantly higher in NOA than in OA group. CAT activity and MDA level correlated negatively with the mean number of retrieved spermatozoa. After cryopreservation, there was a significant increase in CAT activity in OA, while there was no significant difference in MDA level in both OA and positive NOA. We concluded that patients with NOA seem to have increased testicular OS compared to OA. The extent of OS correlated negatively with spermatogenesis grade. Testicular tissue of patients with OA seems to withstand OS induced by cryopreservation more than NOA.

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Section: Introductionmentioning

confidence: 99%

Testicular tissue oxidative stress in azoospermic patients: Effect of cryopreservation

et al. 2020

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“…When no treatable condition is identified, consideration to ICSI with testicular sperm should be given (Figure-1) (24,36). However, given the risks associated with sperm retrieval (37)(38)(39)(40), ICSI with testicular sperm should be reserved for men with confirmed sperm DNA damage or severe oligozoospermia/cryptozoospermia (41); this is one of the reasons why testing is important.…”

Section: Discussionmentioning

confidence: 99%

Are specialized sperm function tests clinically useful in planning assisted reproductive technology?

Esteves

2020

Int. braz j urol.

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40-year-old male patient and 32-year-old female partner, with a history of primary infertility of two years duration. The workup revealed idiopathic mild oligoasthenotheratozoospermia, and no apparent female infertility factors. The couple has failed three intrauterine insemination (IUI) cycles, planning more IUI cycles but also considering in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI).

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“…Nevertheless, sperm retrieval and cryopreservation of testicular spermatozoa should be considered in specific situations of persistent azoospermia when a narrow window of opportunity exists. Sperm retrieval can be performed on an outpatient basis under local/intravenous anesthesia; the procedure is associated with minimal postoperative complications 21 …”

Section: The Impact Of Sars‐cov‐2 For Males In Need Of Sperm Bankingmentioning

confidence: 99%

SARS‐CoV‐2 pandemic and repercussions for male infertility patients: A proposal for the individualized provision of andrological services

Esteves

Lombardo

Garrido³

et al. 2020

Andrology

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The prolonged lockdown of health facilities providing non‐urgent gamete cryopreservation—as currently recommended by many reproductive medicine entities and regulatory authorities due to the SARS‐CoV‐2 pandemic will be detrimental for subgroups of male infertility patients. We believe the existing recommendations should be promptly modified and propose that the same permissive approach for sperm banking granted for men with cancer is expanded to other groups of vulnerable patients. These groups include infertility patients (eg, azoospermic and cryptozoospermic) undergoing medical or surgical treatment to improve sperm quantity and quality, as well as males of reproductive age affected by inflammatory and systemic auto‐immune diseases who are about to start treatment with gonadotoxic drugs or who are under remission. In both scenarios, the “fertility window” may be transitory; postponing diagnostic semen analysis and sperm banking in these men could compromise the prospects of biological parenthood. Moreover, we provide recommendations on how to continue the provision of andrological services in a considered manner and a safe environment. Our opinion is timely and relevant given the fact that fertility services are currently rated as of low priority in most countries.

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Methods of surgical sperm extraction and implications for assisted reproductive technology success

Cited by 30 publications

References 85 publications

Testicular tissue oxidative stress in azoospermic patients: Effect of cryopreservation

Testicular tissue oxidative stress in azoospermic patients: Effect of cryopreservation

Are specialized sperm function tests clinically useful in planning assisted reproductive technology?

SARS‐CoV‐2 pandemic and repercussions for male infertility patients: A proposal for the individualized provision of andrological services

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