Methods of Studying Ultraweak Photon Emission from Biological Objects: I. History, Types and Properties, Fundamental and Application Significance

Naumova, E. V.; Vladimirov, Yu. A.; Beloussov, L. V.; Tuchin, Valery V.; Volodyaev, I.V.

doi:10.1134/s0006350921050158

Cited by 7 publications

(2 citation statements)

References 152 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plants, animals and microbes glow continuously. This spontaneous chemiluminescence (SCL) is attributed to endogenous production of excited states during oxidative metabolic reactions and is therefore closely related to the oxidation status of the emitting organism [ 1 , 2 , 3 , 4 ]. SCL has a very low intensity, typically around 10 −19 to 10 −16 W cm −2 (corresponding to a few hundred photons s −1 cm −2 ), i.e., several orders of magnitude less than bioluminescence which arises from enzymatic reactions in some animals, including fireflies, jellyfishes and fishes [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Imaging of Lipid Peroxidation-Associated Chemiluminescence in Plants: Spectral Features, Regulation and Origin of the Signal in Leaves and Roots

Havaux

Ksas

2022

Antioxidants

View full text Add to dashboard Cite

Plants, like most living organisms, spontaneously emit photons of visible light. This ultraweak endogenous chemiluminescence is linked to the oxidative metabolism, with lipid peroxidation constituting a major source of photons in plants. We imaged this signal using a very sensitive cooled CCD camera and analysed its spectral characteristics using bandpass interference filters. In vitro oxidation of lipids induced luminescence throughout the visible spectrum (450–850 nm). However, luminescence in the red spectral domain (>640 nm) occurred first, then declined in parallel with the appearance of the emission in the blue-green (<600 nm). This temporal separation suggests that the chemical species emitting in the blue-green are secondary products, possibly deriving from the red light-emitting species. This conversion did not seem to occur in planta because spontaneous chemiluminescence from plant tissues (leaves, roots) occurred only in the red/far-red light domain (>640 nm), peaking at 700–750 nm. The spectrum of plant chemiluminescence was independent of chlorophyll. The in vivo signal was modulated by cellular detoxification mechanisms and by changes in the concentration of singlet oxygen in the tissues, although the singlet oxygen luminescence bands did not appear as major bands in the spectra. Our results indicate that the intensity of endogenous chemiluminescence from plant tissues is determined by the balance between the formation of luminescent species through secondary reactions involving lipid peroxide-derived intermediates, including singlet oxygen, and their elimination by metabolizing processes. The kinetic aspects of plant chemiluminescence must be taken into account when using the signal as an oxidative stress marker.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Lipid peroxidation has been identified as a major source of SCL [ 1 , 2 , 3 , 4 , 6 ]. This is particularly true in plants because their lipids are highly unsaturated [ 7 , 8 ] and are therefore very sensitive to oxidation [ 9 ].…”

Section: Introductionmentioning

confidence: 99%