Methods of Pure Culture Study Preliminary Report of the Committee on the Chart for Identification of Bacterial Species

Conn, H. J.; Harding, H. A.; Kligler, I. J.; Frost, W. D.; Prucha, M. J.; Atkins, K. N.

doi:10.1128/jb.3.2.115-128.1918

Cited by 6 publications

(2 citation statements)

References 2 publications

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“…As a substitute sulphanilic acid (or aniline) can be used as the diazo-forming substance, a pink dye being formed on the addition of #-naphthol in absolute alcohol; this has an end point of 1 part in 3,200. Conn, Harding, Kligler, Frost, Prucha, and Atkins (1918), however, advocate the use of sulphanilic acid and c.-naphthol, and it was found that the addition of 1 ml. of 1 % sulphanilic acid in 5N acetic acid and 1 ml.…”

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confidence: 99%

Test for the presence of nitrate not involving carcinogenic reagents

McLean¹,

Henderson²

1966

J Clin Pathol

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The method outlined by McErlean (1963) for concentrating antibodies in serum was modified. In the original technique it was considered necessary to allow the frozen serum to thaw completely during centrifugation. Further experiments have shown that protein and electrolytes can be removed rapidly from frozen serum or urine by centrifugation, at relatively low speeds with little or no visible evidence of thawing.Part of the sample (25 ml.) of urine from a nephritic dog was placed in the barrel of a '20 ml.' plastic syringe suitably plugged at the exit and frozen at -6°C. overnight. A 5 ml. glass bottle was placed in the bottom of a 100 ml. centrifuge bucket and the plastic syringe barrel with frozen urine placed on top after removing the stopper from the exit. Both containers were suitably wrapped. The unit was then centrifuged for 1 minute at 3,500 r.p.m. at room temperature, when 3-47 ml. of yellowish fluid was obtained from the glass bottle and a cylindrical tightly fitting cast of frozen colourless ice was shaken from the syringe barrel.The fluid concentrate and a sample of the original urine were analysed for protein by the Biuret method and for sodiurn using a Beckman spectrophotometer. The ease with which protein and sodium can be removed from frozen urine or serum is due to the fact that water freezes first and the substances suspended or dissolved therein are trapped in the lattice work of ice crystals in a concentrated form and are removed by centrifugal force. A critical temperature must obviously be reached when all the constituents are frozen and this techniquc would not then operate. Technology, 1966). The Griess-Llosvay test detects nitrite when potassium nitrite is present at less than 1 part in 500,O0.

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confidence: 99%

Test for the presence of nitrate not involving carcinogenic reagents

McLean¹,

Henderson²

1966

J Clin Pathol

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“…The 1917 report of this committee (Conn. et al 1918) contained a discussion of methods of pure culture study, designed' primarily to accompany the chart for instruction. Further work has been done on some of these methods during the past year.…”

mentioning

confidence: 99%