Methods of Plant Transformation- A Review

Keshavareddy, G.; Kumar, A.R.V.; Vemanna, Ramu S.

doi:10.20546/ijcmas.2018.707.312

Cited by 53 publications

(25 citation statements)

References 55 publications

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“…They showed resistance only against the viruses for they become transformed but also against several other viruses. Plants defense genes become more adaptive against several viruses when plants are transformed with viral DNA genes Keshavareddy et al (2018). Our studies based on viral DNA genes inoculation.…”

Section: Discussionmentioning

confidence: 98%

Gene Expression Evaluation of Multigenic Cotton (Gossypium hirsutum) against Cotton Leaf Curl Virus

Afzal¹

2021

IJAB

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One of the most crucial threats limiting the sustainable production of cotton is cotton leaf curl disease (CLCuD). There is dire need to produce a resistant variety that can combat CLCuD. For this purpose, virus resistant transgenic cotton plants (MNH-786) with C4 gene construct at T3 generation were selected and sown. Young fresh leaves of multigenic variety of MNH-786 were collected to confirm the transformed construct. Infected whiteflies were used for spreading on transgenic cotton MNH-786 variety with C4 construct to check percentage of infection. Whiteflies were collected from infected cotton plants showing CLCuD and reared in lab to increase the population of whiteflies. After 15 days of feeding, infected leaves of transgenic plants were collected and total DNA of infected leaves of transgenic cotton plant with virus load was extracted. At maturity, data of morphological characteristic was taken from the transgenic cotton plants of MNH-786 and control plants. Resistant transgenic cotton plants showed < 0.5% disease index and recorded more plant height in field condition. Total number of bolls per plant was 20% more in tolerant plants and 40% more in resistant plants as compared to susceptible plants. Molecular analysis of transgenic plants showed clear evidence that expression of construct 4 virus resistant gene against begomoviruses in resistant and tolerant group of transgenic plants was more as compared to susceptible group and control. © 2021 Friends Science Publishers

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Section: Discussionmentioning

confidence: 98%

Gene Expression Evaluation of Multigenic Cotton (Gossypium hirsutum) against Cotton Leaf Curl Virus

Afzal¹

2021

IJAB

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“…Agrobacterium-mediated transformation approach and particle bombardment (biolistic) approach [25][26][27]. Although biolistic was commonly used to express heterologous gene(s) in recalcitrant plant species [27], Agrobacterium-mediated transformation system was preferred due to its simplicity, efficiency and cost effectiveness [28].…”

Section: The Most Common and Extensively Developed Genetic Transformamentioning

confidence: 99%

Interactomics: Development of an efficient and improved Agrobacterium tumefaciens-mediated transformation method for transient expression of heterologous protein in recalcitrant plant tissues in planta

Affandi¹,

Mostaffa²,

Al-Idrus³

2020

Preprint

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Background: Since more researches leaned towards a holistic approach of "omics" to understand PPI, interactomics studies pivoting on AP-MS approach are getting popular amongst plant scientists.However, to date, AP-MS approach has yet to be adopted to study plant-pathogen interaction even in model plants, hence, the needs to design and develop an optimised transformation procedure for successful isolation of pathogen-targeted plant proteins.Methods: Banana plants were grown in soil or hydroponic media for 2-3 months before subjected to transformation procedure. As a bait, m16D10 , a mature parasitism gene isolated from Meloidogyne incognita was transiently expressed in banana root tissues cv. Grand naine (ITC1256) to prey for protein interactions to uncover mechanisms of host cell rewiring. Current opinion in microbiology 2017, 39:7-15. 21.Olson MG, Ouellette SP, Rucks EA: A meta-analysis of affinity purification-mass spectrometry experimental systems used to identify eukaryotic and chlamydial proteins at the Chlamydia trachomatis inclusion membrane. Efficient transient protein expression in tomato cultivarsand wild species using agroinfiltration-mediated high expression system. Plant cell reports 2019, 38(1):75-84. 30. Khanna HK, Raina SK: Agrobacterium-mediated transformation of indica rice cultivars using binary and superbinary vectors. Functional Plant Biology 1999, 26(4):311-324. 31. Rodríguez M, Ramírez NI, Ayala M, Freyre F, Pérez L, Triguero A, Mateo C, Selman-Housein G, Gavilondo JV, Pujol M: Transient expression in tobacco leaves of an aglycosylated recombinant antibody against the epidermal growth factor receptor. Biotechnology and bioengineering 2005, 89(2):188-194. 32. Tsuda K, Qi Y, Nguyen LV, Bethke G, Tsuda Y, Glazebrook J, Katagiri F: An efficient Agrobacterium-mediated transient transformation of Arabidopsis. The Plant Journal 2012, 69(4):713-719. 33. Zhang X, Henriques R, Lin S-S, Niu Q-W, Chua N-H: Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nature protocols 2006, 1(2):641. 34. Lee MW, Yang Y: Transient expression assay by agroinfiltration of leaves. In: Arabidopsis Protocols. Springer; 2006: 225-229. 35. Zhang Y, Natale R, Domingues AP, Toleco MR, Siemiatkowska B, Fàbregas N, Fernie AR: Rapid Identification of Protein-Protein Interactions in Plants. Current protocols in plant biology 2019, 4(4):e20099. 36. Wang W, Vignani R, Scali M, Cresti M: A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. 24 Electrophoresis 2006, 27(13):2782-2786. 37. Ke J, Khan R, Johnson T, Somers D, Das A: High-efficiency gene transfer to recalcitrant plants by Agrobacterium tumefaciens. Plant Cell Reports 2001, 20(2):150-156. 38. Clement WKF, Lai KS, Wong MY, Maziah M: Heat and hydrolytic enzymes treatment improved the Agrobacterium-mediated transformation of recalcitrant indica rice (Oryza sativa L.). Plant Cell, Tissue and Organ Culture (PCTOC) 2016, 125(1):183-190. 39. Huang G, Dong R, Allen R, Davis EL, Baum T...

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“…Plant transformation is now a core research tool in plant biotechnology, it is a practical tool for transgenic plant development that is done by several techniques for transfer of isolated genetic materials into a viable host cell. (17). Agrobacterium tumefaciens is a soil phytopathogen that naturally infects plant wound sites and causes crown gall disease via delivery of transferred T-DNA from bacterial cells into host plant cells through its Ti plasmid.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-Mediated Transformation of Two Tomato Cultivars (Lycopersicon Esculentum Mill.) Cv. Sandra and Rocky

Danial¹,

Ibrahim²,

Song³

2021

Iraqi J. Agric. Sci.

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An efficient protocol for Agrobacterium-mediated transformation of tomato cultivars Sandra and Rocky was conducted to examine the possibility of producing transgenic tomato plants cultivars harbouring the nptII gene, conferring kanamycin resistance. To achieve this aim, tomato cotyledon explants were transformed using EHA105 Agrobacterium tumefaciens strain harboring the binary vectors pBI121 which contains Gus gene, and neomycin phosphotransferase II (nptII) as selectable marker gene under the control of a CaMV35S promoter and nopaline synthase (nos) Terminator. Transformant detection was carried out in three distinct ways. First antibiotic selection, Kanamycin at a concentration of100 mgl-1 found to be efficient for this purpose. Second histochemical GUS assay revealed the presence of blue colored zones in a number of shoots and leaves for both in vitro and the greenhouse-grown transgenic plants. Third PCR analysis indicated positive result by showing the fragment for nptII gene in tested transformants, while was absent in non-transgenic control (wild type). On the other hand, the results showed that Sandra cultivar was more efficient for regeneration and subsequently transformation frequency than Rocky cultivar, which record 26.66% of transformation frequency compared with 11.57% in Rocky cultivar.

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Methods of Plant Transformation- A Review

Cited by 53 publications

References 55 publications

Gene Expression Evaluation of Multigenic Cotton (Gossypium hirsutum) against Cotton Leaf Curl Virus

Gene Expression Evaluation of Multigenic Cotton (Gossypium hirsutum) against Cotton Leaf Curl Virus

Interactomics: Development of an efficient and improved Agrobacterium tumefaciens-mediated transformation method for transient expression of heterologous protein in recalcitrant plant tissues in planta

Agrobacterium-Mediated Transformation of Two Tomato Cultivars (Lycopersicon Esculentum Mill.) Cv. Sandra and Rocky

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