Methods of Isolation and Estimation of Sterols

Cook, Robert P.; Rattray, J. B. M.

doi:10.1016/b978-1-4832-2772-6.50008-5

Cited by 6 publications

(4 citation statements)

References 119 publications

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“…Development in the first dimension was with chloroform and in the second dimension with hexane-ethyl acetate (80:20). Compounds of interest were revealed after spraying with either Lieberman-Burchard reagent (7) or 50% aqueous H2SO4 and heating at 100 C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Fatty Acid and Sterol Composition of Mucor genevensis in Relation to Dimorphism and Anaerobic Growth

1971

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Fatty acid and sterol content and composition were determined for the dimorphic mold, Mucor genevensis, grown under a variety of experimental conditions. Fatty acids account for 6 to 9% of the dry weight of aerobically grown mycelium, and 70 to 80% of these are unsaturated. The organism contains 'y-linolenic acid which is characteristic for Phycomycetes, and in sporangiospores this compound represents 40% of the total fatty acids. Of the sterols found in mycelium, 80% is ergosterol, and stigmasterol was positively identified as one of the minor components. In anaerobically grown yeastlike cells, sterol content is less than 10% of the level found in aerobically grown cells, and fatty acids amount to less than 2% of the dry weight. These fatty acids are predominantly short chain and less than 10% are unsaturated. Yeastlike cells obtained under aerobic conditions by growth in the presence of phenethyl alcohol have fatty acid and sterol compositions characteristic of aerobically grown mycelium. It is concluded that the dimorphology of the organism is not directly related to lipid composition.

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Section: Methodsmentioning

confidence: 99%

Fatty Acid and Sterol Composition of Mucor genevensis in Relation to Dimorphism and Anaerobic Growth

1971

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“…The possibility of specific interrelationships in such changes in drug-resistance of Chilomonas is being investigated. So far, it appears that adaptation to isoniazid is accompanied by increased susceptibility to sulfanilamide ( 6 ) , much as in the case of adaptation to PABA. Unpublished data indicate further that a sulfonamide-resistant strain (adapted to 700 mg/100 ml) is more susceptible than a normal strain to isoniazid.…”

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confidence: 99%

“…been reported by Seaman(l9). The L-B reaction is positive with non-sterols such as carotene, linoleic acid, etc (6)…”

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confidence: 99%

Lipid and Sterol Content of Some Protozoa*

Aaronson

Baker

1961

The Journal of Protozoology

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Total lipids of several protozoa were determined and found higher than in most other microorganisms. The lipid content in a phytoflagellate, Ochromonas malhamensis, could be increased by excess nicotinic acid or deficiency in nitrogen supply. Cell multiplication and lipid synthesis were unaffected by inhibitors of vitamin synthesis such as penicillamine, pan‐toyltaurine, or pyridine‐3‐sulfonic acid, up to 100 mg%. The phytoflagellates, O. danica, O. malhamensis, Euglena gracilis, and the photosynthetic bacterium Chlorobium limkola contained large quantities of non‐saponifiable lipids: ergesterol was identified as a major component of this lipid fraction. Sterols were not found in the bacteria, Streptococcus faecalis, Rhodopseudomonas palustris; the algae. Cyanidium calidarnm. Anacystis nidulans, and Porphyridium cruentum; and the protozoan Tetrahymena pyriformis.

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“…The technique of micropuncture has yielded information on the contents of proximal regions of the epididymis of rats (Levine & Marsh, 1971;Hinton, Setchell & White, 1976;Turner, Hartmann & Howards, 1977;Levine & Kelly, 1978) and hamsters (Warren, 1959) after hydrolysis in 0-1 m-H2S04 for 1 h at 80°C. Cholesterol was measured by the Liebermann-Burchard reaction (Cook & Rattray, 1958 …”

Section: Introductionmentioning

confidence: 99%

Investigation by luminal perfusion of the transfer of compounds into the epididymis of the anaesthetized rat

Cooper¹,

Waites²

1979

Reproduction

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Summary. Controlled perfusion through the lumen of the distal cauda epididymidis in the anaesthetized rat has been explored as a means of examining physiological exchanges from blood across the epididymal epithelium. The mean length of the perfused, sperm-free, tubule was 14\m=.\5cm (\m=+-\1\m=.\5s.e.m., n = 9). No cholesterol, protein or sialic acid was detected in the perfusate at flow rates exceeding 10 \g=m\l /mi n, but at rates of 0\ m=. \ 4\ p=n-\ 1\ m=. \ 2 \g=m\l/min,protein appeared at concentrations of 0\m=.\21\p=n-\0\m=.\55 mg/ml (i.e. secretion rates of 0\m=.\21\p=n-\0\m=.\83 \g=m\g/min; 3 rats). Glucose was detected at all perfusion rates (3\p=n-\27\g=m\l/min)at concentrations of 0\m=.\06\p=n-\0\m=.\58 mM (0\ m=. \ 8\ p=n-\ 6\ m=. \ 8% blood levels). This evidence suggests that the preparation is physiological and could be used to explore the dynamics of exchanges between blood and epididymis.

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Methods of Isolation and Estimation of Sterols

Cited by 6 publications

References 119 publications

Fatty Acid and Sterol Composition of Mucor genevensis in Relation to Dimorphism and Anaerobic Growth

Fatty Acid and Sterol Composition of Mucor genevensis in Relation to Dimorphism and Anaerobic Growth

Lipid and Sterol Content of Some Protozoa*

Investigation by luminal perfusion of the transfer of compounds into the epididymis of the anaesthetized rat

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