Methods of Determination of Antibiotic Residues in Honey

Jakšić, Sandra; Ratajac, Radomir; Prica, Nadežda; Apić, Jelena; Ljubojević, Dragana; Stošić, Marina Žekić; Baloš, Milica Živkov

doi:10.1134/s1061934818040044

Cited by 16 publications

(6 citation statements)

References 43 publications

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“…Screening methods are the first step in controlling antibiotic residues in food (Gaudin, 2017;ANSES, 2019;AFNOR, 2014). They can detect the presence of an antibiotic or group of antibiotics at the level of interest, and usually provide qualitative results (Jakšić et al, 2018). Then, in a second step the residues of the positively tested samples are quantified mostly by quantitative confirmation methods such as using an analytical method based on high performance liquid chromatography associated with a mass detector (HPLC-MS/MS) (Gaudin, 2017;Jakšić et al, 2018;Laurentie et al, 2002;Kaufmann et al, 2002).…”

Section: Relationship Among Antibiotic Residues and Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O Berg) Frommentioning

confidence: 99%

Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

et al. 2020

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Antibiotic-resistant bacteria continue to be of major health concern worldwide. In recent years, several reports and scientific articles claim the contamination of honey by antibiotics, detectable concentrations of antibiotic residues in honey are illegal. They, may cause hypersensitivity or resistance to drug therapy in humans, and are perceived by consumers as undesirable. In this sense, the purpose of this work was to examine the antibacterial activity of the Euphorbia resinifera (E. resinifera) honey against Escherichia coli and Staphylococcus aureus in vitro using the well-agar diffusion assay followed by dilution range to obtain more precise minimum inhibitory concentration values. The second aim is to evaluate the presence of antibiotics in honey using a screening test: Evidence InvestigatorTM, an immuno-enzymatic method for detection of 27 antibiotic residues followed by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) for confirmation of suspect samples; in order to assess the relationship between the presence of antibiotic residues and the antibacterial activity of honey. In this study, a total of 37 E. resinifera honey samples were analyzed. The results show that all samples of honey inhibited the growth of bacteria at the dilutions at 50% (v/v); the highest inhibition zone (25.98 ± 0.11 mm) was recorded from sample 5 for Staphylococcus aureus and (13.84 ± 1.10 mm) in sample 17 for Escherichia coli and that 50% (v/v) dilutions showed significant antibacterial effect compared to other dilutions (6.25, 12.5, 25% (v/v)). In all samples, there were no antibiotic residues detected except for one showing the detection of Trimethoprim at 6.48 µg kg-1. Our research is one of the first studies that relate the he relationship between the presence of antibiotic residues and the antibacterial activity of Euphorbia resinifera honey and showed that the antibacterial activity of honey might be due to the high osmotic nature, a low pH, its content of phenolic compounds and hydrogen peroxide and also to its content of methylglyoxal.

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Section: Relationship Among Antibiotic Residues and Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O Berg) Frommentioning

confidence: 99%

Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

et al. 2020

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“…There are two general types of honey: nectar and honeydew (Bogdanov 2016). Since it is widely used for both nutritional and medicinal purposes (Abeshu and Geleta 2016), honey should be free from any contamination (Jakšić et al 2018). Honey contamination is promoted by collection of nectar from a polluted environment and management of honeybee colonies during honey production and after honey collection (Bogdanov et al 2003;Bogdanov 2006).…”

Section: Introductionmentioning

confidence: 99%

Capacity of honeybees to remove heavy metals from nectar and excrete the contaminants from their bodies

et al. 2021

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We examined the residues of 13 elements in soil, plant parts, nectar, bee heads, thorax, and abdomens, feces from bee guts, and bee products sampled from two Polish cities (Lublin and Poznań). Our findings indicated that bees have an extraordinary ability to remove metals from nectar when converting nectar into honey. Compared to nectar, honey contained 40-fold lower Fe, 26-fold lower Zn, and eightfold lower Cu and Cd levels, indicating removal of these elements via nectar processing, during which water is evaporated and complex sugars are decomposed into simple ones. The amount of Pb remained unchanged; however, it can also be regarded as a fourfold decrease due to water evaporation from honey, compared to nectar. Some portion of the ingested Fe, Cu, and Zn was used by bees, and the excess amounts were excreted in feces. All analyzed elements were present as biocomplexes transported from the alimentary tract through the abdomen to the thorax and head. Elements transferred in the alimentary tract were partially immobilized/metabolized in the bee fat body, and their residues were excreted with feces from the gut. We postulate that honey is not a good indicator of environmental pollution, as a high amount of elements is removed by bees from their bodies.

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“…Human lifespan dramatically increased due to the wide use of these compounds 2,3 . However, its unwise use has led to the dissemination of these compounds and corresponding metabolites in theecosystem 4 and food 5 (in particular of animal origin such as milk, 6‐9 meat, 10 fish, 11 and honey 12,13 ). This enhances the already worrying levels of bacterial resistance 14 .…”

Section: Introductionmentioning

confidence: 99%

“…Antibiotic determination can be achieved through a multitude of methods like immunoassays, 11,13 chromatographic methods, 13,18 or capillary electromigration 19,20 ; all have inherent advantages and disadvantages. Electroanalytical assays are a suitable alternative when seeking simplicity, portability, speed, low‐cost and sensitivity, 6,21,22 however, the lack of selectivity is a recurrent issue.…”

Section: Introductionmentioning

confidence: 99%

Employing molecularly imprinted polymers in the development of electroanalytical methodologies for antibiotic determination

Benachio

Lobato

Gonçalves

2020

J of Molecular Recognition

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Antibiotics, although being amazing compounds, need to be monitored in the environment and foodstuff. This is primarily to prevent the development of antibiotic resistance that may make them ineffective. Unsurprisingly, advances in analyticalsciences that can improve their determination are appreciated. Electrochemical techniques are known for their simplicity, sensitivity, portability and low‐cost; however, they are often not selective enough without recurring to a discriminating element like an antibody. Molecular imprinting technology aims to create artificial tissues mimicking antibodies named molecularly imprinted polymers (MIPs), these retain the advantages of selectivity but without the typical disadvantages of biological material, like limited shelf‐life and high cost. This manuscript aims to review all analytical methodologies for antibiotics, using MIPs, where the detection technique is electrochemical, like differential pulse voltammetry (DPV), square‐wave voltammetry (SWV) or electrochemical impedance spectroscopy (EIS). MIPs developed by electropolymerization (e‐MIPs) were applied in about 60 publications and patents found in the bibliographic search, while MIPs developed by other polymerization techniques, like temperature assisted (“bulk”) or photopolymerization, were limited to around 40. Published works covered the electroanalysis of a wide range of different antibiotics (β‐lactams, tetracyclines, quinolones, macrolides, aminoglycosides, among other), in a wide range of matrices (food, environmental and biological).

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Methods of Determination of Antibiotic Residues in Honey

Cited by 16 publications

References 43 publications

Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

Capacity of honeybees to remove heavy metals from nectar and excrete the contaminants from their bodies

Employing molecularly imprinted polymers in the development of electroanalytical methodologies for antibiotic determination

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