Methods in Comprehensive Mass Spectrometry-Based Measurement of Sphingolipids

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

“…Sulfatide was extracted using hexane/isopropanol and saponified to lysosulfatide with sodium hydroxide. After purification, the samples were desalted by Mono-tip C18 cartridges (GL Sciences) followed by the addition of N-acetyl lysosulfatide containing d18:0-sphinganine (97) (Farwanah et al, 2011) Lipidomics of glycosphingolipids, structure and analysis 166 (Farwanah & Kolter, 2012) Chip-based nanoelectrospray mass spectrometry of brain gangliosides 93 Methods in comprehensive mass spectrometry-based measurement of sphingolipids 28 (Gassler, 2012) Thin-layer chromatography and mass spectrometry of glycosphingolipids 228 (Meisen et al, 2011) Sphingolipid and glycosphingolipid metabolic pathways 639 (Merrill, 2011) Analysis of mammalian sphingolipids by LC/MS and tissue imaging 77 (Sullards et al, 2011) High-performance TLC/MS for analysis of neutral glycosphingolipids 41 ( A new method for releasing free reducing glycans from GSLs involves a brief treatment with ozone followed by heating in aqueous buffer at 658C for 16 hr. The procedure appeared to be highly efficient with no evidence of peeling reactions and the released glycans could be permethylated or tagged with a fluorescent label for application of traditional analytical methods (Song et al, 2012a).…”