Methods for Two-Dimensional Cell Confinement

Berre, Maël Le; Zlotek-Zlotkiewicz, Ewa; Bonazzi, Daria; Lautenschlaeger, Franziska; Piel, Matthieu

doi:10.1016/b978-0-12-800281-0.00014-2

Cited by 81 publications

(89 citation statements)

References 8 publications

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“…Vertical confinement can simulate migration spaces observed between opposing surfaces in the body, such as in the space between peritoneal layers covering organs (1), and can induce blebbing migration observed in vivo through the peritumoral ECM (15, 83). Vertical confinement assays made possible by microfabrication techniques enable cell confinement to heights down to 3 μm, with an accuracy of 100 nm over large areas (square centimeters) (Figure 1) (84). In this technique, a pillared PDMS array is lowered over a bed of cells, and cells dispersed between the pillars are vertically confined to a height defined by the height of the pillars (84).…”

Section: Experimental Models For Studying Cell Behavior In Confinementioning

confidence: 99%

“…Vertical confinement assays made possible by microfabrication techniques enable cell confinement to heights down to 3 μm, with an accuracy of 100 nm over large areas (square centimeters) (Figure 1) (84). In this technique, a pillared PDMS array is lowered over a bed of cells, and cells dispersed between the pillars are vertically confined to a height defined by the height of the pillars (84). This is a very high throughput technique if true 3D confinement is deemed unnecessary and vertical or so-called sandwich confinement is sufficient, and it is readily adapted to multiwall plates (84).…”

Section: Experimental Models For Studying Cell Behavior In Confinementioning

confidence: 99%

“…In this technique, a pillared PDMS array is lowered over a bed of cells, and cells dispersed between the pillars are vertically confined to a height defined by the height of the pillars (84). This is a very high throughput technique if true 3D confinement is deemed unnecessary and vertical or so-called sandwich confinement is sufficient, and it is readily adapted to multiwall plates (84). Application of this technique has demonstrated how confinement can lead to rupture of the nuclear lamina and modulate gene expression pathways (85) and how mesenchymal cells can spontaneously switch to a rapid amoeboid migration mode when vertically confined (15).…”

Section: Experimental Models For Studying Cell Behavior In Confinementioning

confidence: 99%

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Engineered Models of Confined Cell Migration

Paul¹,

Hung²,

Wirtz

et al. 2016

Annu. Rev. Biomed. Eng.

111

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Cells in the body are physically confined by neighboring cells, tissues, and the extracellular matrix. Although physical confinement modulates intracellular signaling and the underlying mechanisms of cell migration, it is difficult to study in vivo. Furthermore, traditional two-dimensional cell migration assays do not recapitulate the complex topographies found in the body. Therefore, a number of experimental in vitro models that confine and impose forces on cells in well-defined microenvironments have been engineered. We describe the design and use of microfluidic microchannel devices, grooved substrates, micropatterned lines, vertical confinement devices, patterned hydrogels, and micropipette aspiration assays for studying cell responses to confinement. Use of these devices has enabled the delineation of changes in cytoskeletal reorganization, cell–substrate adhesions, intracellular signaling, nuclear shape, and gene expression that result from physical confinement. These assays and the physiologically relevant signaling pathways that have been elucidated are beginning to have a translational and clinical impact.

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Section: Experimental Models For Studying Cell Behavior In Confinementioning

confidence: 99%

Section: Experimental Models For Studying Cell Behavior In Confinementioning

confidence: 99%

Section: Experimental Models For Studying Cell Behavior In Confinementioning

confidence: 99%

See 1 more Smart Citation

Engineered Models of Confined Cell Migration

Paul¹,

Hung²,

Wirtz

et al. 2016

Annu. Rev. Biomed. Eng.

111

View full text Add to dashboard Cite

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“…The assayed endpoints of these studies can be broadly grouped into four categories:(i) the study of individual sub-processes which are a part of cell motility [130] (e.g., actin organization and dynamics, MT guidance to FAs, etc. described in Section 6.1 of this Review); (ii) assays for the mechanical properties of stationary and motile cells [131] (described in Section 6.3) ; (iii) systems probing the overall cell motility within various geometric confines [132,133] (described in Section 6.2 and 6.3.3); and (iv) systems where cell motility is studied under conditions that in part mimic in vivo microenvironment [30,31] (described in Section 6.4 and 6.5).…”

Section: Connection To Current Microtechnologymentioning

confidence: 99%

“…More recently, biomechanical/biophysical characteristics of cells, such as traction forces (TFs) exerted upon substrate,(reviewed in [131] and [202] ), 3D cell shape, [168] and cell responses to challenging/constrained 3D environments [132, 133, 203] and varying substrate stiffness [131, 204] have been studied as potential biomarkers for metastatic potential. Various microfabricated substrates and tools reviewed below have been developed to enable quantitative characterization of mechanical properties of cancerous and non-cancerous the cells.…”

Section: Probing Cytoskeletal Structures Cell Mechanics and Cell mentioning

confidence: 99%

Microfabricated Systems and Assays for Studying the Cytoskeletal Organization, Micromechanics, and Motility Patterns of Cancerous Cells

Huda

Pilans

Makurath

et al. 2014

Adv Materials Inter

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Cell motions are driven by coordinated actions of the intracellular cytoskeleton – actin, microtubules (MTs) and substrate/focal adhesions (FAs). This coordination is altered in metastatic cancer cells resulting in deregulated and increased cellular motility. Microfabrication tools, including photolithography, micromolding, microcontact printing, wet stamping and microfluidic devices have emerged as a powerful set of experimental tools with which to probe and define the differences in cytoskeleton organization/dynamics and cell motility patterns in non-metastatic and metastatic cancer cells. In this review, we discuss four categories of microfabricated systems: (i) micropatterned substrates for studying of cell motility sub-processes (for example, MT targeting of FAs or cell polarization); (ii) systems for studying cell mechanical properties, (iii) systems for probing overall cell motility patterns within challenging geometric confines relevant to metastasis (for example, linear and ratchet geometries), and (iv) microfluidic devices that incorporate co-cultures of multiple cells types and chemical gradients to mimic in vivo intravasation/extravasation steps of metastasis. Together, these systems allow for creating controlled microenvironments that not only mimic complex soft tissues, but are also compatible with live cell high-resolution imaging and quantitative analysis of single cell behavior.

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Molecular cancer cell responses to solid compressive stress and interstitial fluid pressure

2021

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Alterations to the mechanical properties of the microenvironment are a hallmark of cancer. Elevated mechanical stresses exist in many solid tumors and elicit responses from cancer cells. Uncontrolled growth in confined environments gives rise to elevated solid compressive stress on cancer cells. Recruitment of leaky blood vessels and an absence of functioning lymphatic vessels causes a rise in the interstitial fluid pressure. Here we review the role of the cancer cell cytoskeleton and the nucleus in mediating both the initial and adaptive cancer cell response to these two types of mechanical stresses. We review how these mechanical stresses alter cancer cell functions such as proliferation, apoptosis, and migration.

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Methods for Two-Dimensional Cell Confinement

Cited by 81 publications

References 8 publications

Engineered Models of Confined Cell Migration

Engineered Models of Confined Cell Migration

Microfabricated Systems and Assays for Studying the Cytoskeletal Organization, Micromechanics, and Motility Patterns of Cancerous Cells

Molecular cancer cell responses to solid compressive stress and interstitial fluid pressure

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