Methods for the Preparation of Purified Granulopoiesis-Inhibiting Factor (Chalone)

Paukovits, Walter R.; Lærum, Ole Didrik; Paukovits, Johanna B.; Hinterberger, W; Rogan, Alfred M.

doi:10.1515/bchm2.1983.364.1.383

Cited by 11 publications

(1 citation statement)

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“…In addition, highly purified peptide from natural sources was used. This was obtained by fractionating conditioned medium from 10 human buffycoats on a Sephadex G25-fine column, followed by further fractionation on Sephadex G10 and cation exchange chromatography on Dowex 1 x 2 (22,23). Biological activity was determined by testing a dilution series on myelopoietic colony formation in agar (Batches 28/76 E and 2/82 B).…”

Section: Methodsmentioning

confidence: 99%

Effects of a hemoregulatory peptide (HP5b) on erythroid and myelopoietic colony formation in vitro

Kreja

Hågå

Müller‐Bérat

et al. 1986

Scandinavian Journal of Haematology

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A hemoregulatory peptide (HPSb) associated with mature human granulocytes has been investigated for inhibitory effects on murine hemopoietic stem cells in vitro. Both highly purified peptide from natural sources and a synthetic analog peptide have been investigated in parallel. Strong inhibitory effects were found on myelopoietic colony formation in the dose range 10-13-10-s mol/l. On exceeding this dose, the inhibitory effect disappeared. Moderate to slight inhibitory effects on erythroid colony formation (BFU-E and CFU-E) from adult animals were only seen in 1000 x the optimal doses for myelopoiesis. No effect was found on CFU-E from fetal liver. The peptide thus has a selective effect on myelopoiesis in a certain dose range. A regulatory mechanism for the peptide on hemopoiesis is proposed.

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Section: Methodsmentioning

confidence: 99%