Methods for the Experimental Evaluation of Neuromuscular Blocking Agents

Харкевич, Д. А.

doi:10.1007/978-3-642-70682-0_11

Cited by 2 publications

(2 citation statements)

References 52 publications

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“…Kharkevich, 1986). The preparations were set up in a 25 ml organ bath through which the solutions flowed continuously with the aid of a roller pump.…”

Section: Methodsmentioning

confidence: 99%

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1,3,8‐ and 1,3,7‐substituted xanthines: relative potency as adenosine receptor antagonists at the frog neuromuscular junction

Sebastião

Ribeiro

1989

British J Pharmacology

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1 The ability of 1,3,8-substituted xanthines (1,3-dipropyl-8-(4(2-aminoethyl)amino) carbonylmethyloxyphenyl)xanthine (XAC), 1,3-dipropyl-8(4-carboxymethyloxyphenyl)xanthine (XCC), 1,3-dipropyl-8-2-amino-4-chlorophenyl)xanthine (PACPX), 1,3-dipropyl-8-cyclopentylxanthine (DPCPX), 1,3-diethyl-8-phenylxanthine (DPX) and 8-phenyltheophylline (8-PT)), of 1,3,7-substituted xanthines (1-propargyl-3,7-dimethylxanthine (PGDMX) and caffeine), and of a 3-substituted xanthine (enprofylline) to antagonize the inhibitory effect of 2-chloroadenosine (CADO) on the amplitude of nerve-evoked twitches was investigated in innervated sartorius muscles of the frog. 2 All the 1,3,8-substituted xanthines, in concentrations virtually devoid of effect on neuromuscular transmission, shifted to the right, in a near parallel manner the log concentration-response curve for CADO. Linear Schild plots with slopes near to unity at concentration-ratios less than 14 were obtained for XAC, XCC, DPCPX, DPX and 8-PT. 4 The 1,3,7-substituted xanthines, PGDMX and caffeine, in concentrations virtually devoid of effect on neuromuscular transmission, also caused parallel shifts to the right of the log concentration-response curves for CADO, but were less potent than the 1,3,8-substituted xanthines. PGDMX was more than 20 times more potent than caffeine. 5 Enprofylline in concentrations up to 100pM did not antagonize the inhibitory effect of CADO on neuromuscular transmission.6 It is concluded that the antagonist profile of the adenosine receptor mediating inhibition of transmission at the frog neuromuscular junction is different from the antagonist profile of the A1-and A2-adenosine receptors.

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“…Kharkevich, 1986). The preparations were set up in a 25 ml organ bath through which the solutions flowed continuously with the aid of a roller pump.…”

Section: Methodsmentioning

confidence: 99%

“…The experiments were carried out at room temperature (22-250C) on innervated sartorius muscles of the frog (Rana ridibunda) (see e.g. Kharkevich, 1986). The preparations were set up in a 25 ml organ bath through which the solutions flowed continuously with the aid of a roller pump.…”

Section: Methodsmentioning

confidence: 99%

1,3,8‐ and 1,3,7‐substituted xanthines: relative potency as adenosine receptor antagonists at the frog neuromuscular junction

Sebastião

Ribeiro

1989

British J Pharmacology

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Monitoring of the neuromuscular transmission by electromyography (I). Stability and temperature dependence of evoked EMG response compared to mechanical twitch recordings in the cat

Engbaek

Skovgaard

Friis

et al. 1992

Acta Anaesthesiol Scand

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The stability over time and the effect of muscle temperature change were evaluated for the evoked compound EMG and for the mechanomyogram of the tibialis anterior muscle of 7 anaesthetized cats. Both EMG areas and amplitudes were recorded. During stimulation for 3 h with 0.1 Hz (one leg) and train-of-four (TOF) (the other leg), the EMG was stable while the mechanomyogram initially increased 35-50% in the first 7-8 min and then decreased 19-22% and 5-8% over the first and second 1.5-h period, respectively. During subsequent mean muscle temperature reduction to 28.8 degrees C (0.1 Hz) and 29.7 degrees C (TOF) and rewarming, an inverse linear relationship was found between temperature and both the EMG and the mechanomyogram. During temperature reduction EMG increased about 6% (areas) and 2% (amplitudes) per degrees C. During rewarming, parameters decreased about 4.5% and 2% per degrees C, respectively (P less than 0.05 comparing EMG areas during cooling and rewarming). TOF ratio of the EMG was not affected by temperature. A very large interindividual variation was observed for the effect of temperature on the mechanomyogram with changes ranging up to 15% per degrees C for some cats. TOF ratio of the mechanomyogram was reduced from 1.02 to 0.94 at lowest muscle temperature. It is concluded that the evoked EMG may be preferable to the mechanomyogram in cat experiments investigating the neuromuscular transmission.

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Methods for the Experimental Evaluation of Neuromuscular Blocking Agents

Cited by 2 publications

References 52 publications

1,3,8‐ and 1,3,7‐substituted xanthines: relative potency as adenosine receptor antagonists at the frog neuromuscular junction

1,3,8‐ and 1,3,7‐substituted xanthines: relative potency as adenosine receptor antagonists at the frog neuromuscular junction

Monitoring of the neuromuscular transmission by electromyography (I). Stability and temperature dependence of evoked EMG response compared to mechanical twitch recordings in the cat

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