Methods for the determination of limit of detection and limit of quantitation of the analytical methods

Abstract

The quality of an analytical method developed is always appraised in terms of suitability for its intended purpose, recovery, requirement for standardization, sensitivity, analyte stability, ease of analysis, skill subset required, time and cost in that order. It is highly imperative to establish through a systematic process that the analytical method under question is acceptable for its intended purpose. Limit of detection (LOD) and limit of quantification (LOQ) are two important performance characteristics in method validation. LOD and LOQ are terms used to describe the smallest concentration of an analyte that can be reliably measured by an analytical procedure. There has often been a lack of agreement within the clinical laboratory field as to the terminology best suited to describe this parameter. Likewise, there have been various methods for estimating it. The presented review provides information relating to the calculation of the limit of detection and limit of quantitation. Brief information about differences in various regulatory agencies about these parameters is also presented here. Key words:Detection limit, limit of detection, limit of quantitation, quantitation limit, methods for determination of LOD and LOQ Limit of detection (LOD) and limit of quantitation (LOQ) parameters are related but have distinct definitions and should not be confused. The intent is to define the smallest concentration of analyte that can be detected with no guarantee about the bias or imprecision of the result by an assay, the concentration at which quantitation as defined by bias and precision goals is feasible, and finally the

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“…34,35 The limit of quantification, LoQ, was calculated as 10 standard deviation of the negative control. 34,35 …”

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“…Results were expressed as micrograms per liter (μg/L) for seawater and milligram per kilogram (mg/kg) for fish tissues. The LOD was estimated based on work by Shavarista and her group [10]. Table 1 shows the values of in situ of basic water quality parameters measured using multi probes meter from eight sampling locations.…”