Methods for Studying the Distribution of Pigment in Malpighian Tubules of Drosophila Melanogaster

Hartmann-Goldstein, I. J.; Koliantz, Gregor; Hoyland, M

doi:10.3109/10520297609116682

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…3A); indeed, no line marked all the principal cells of the main segment. Interestingly, mutations at the white eye color locus also affect tubule color, and a similar mosaicism in principal cell color has been described in those white mutants that show position effect variegation of eye pigmentation (30,31). Although position effect variegation is not the cause of our own observations, both lines of evidence demonstrate differences in otherwise indistinguishable cells with respect to transcriptional properties and thus presumably with respect to function.…”

Section: Resultsmentioning

confidence: 38%

Functional domains are specified to single-cell resolution in a Drosophila epithelium

Sözen

Armstrong²,

Yang³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

170

165

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Specification of pattern is fundamental to the development of a multicellular organism. The Malpighian (renal) tubule of Drosophila melanogaster is a simple epithelium that proliferates under the direction of a single tip cell into three morphologically distinct domains. However, systematic analysis of a panel of over 700 P{GAL4} enhancer trap lines reveals unexpected richness for such an apparently simple tissue. Using numerical analysis, it was possible formally to reconcile apparently similar or complementary expression domains and thus to define at least five genetically defined domains and multiple cell types. Remarkably, the positions of domain boundaries and the numbers of both principal and secondary (''stellate'') cell types within each domain are reproducible to near single-cell precision between individual animals. Domains of physiological function were also mapped using transport or expression assays. Invariably, they respect the boundaries defined by enhancer activity. These genetic domains can also be visualized in vivo, both in transgenic and wild-type f lies, providing an ''identified cell'' system for epithelial physiology. Building upon recent advances in Drosophila Malpighian tubule physiology, the present study confirms this tissue as a singular model for integrative physiology.

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Section: Resultsmentioning

confidence: 38%

Functional domains are specified to single-cell resolution in a Drosophila epithelium

Sözen

Armstrong²,

Yang³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

170

165

View full text Add to dashboard Cite

show abstract

Modification of gene suppression in Drosophila melanogaster by sex chromosomes: I. Effect of the Y chromosome on variegation in malpighian tubules of wm4 larvae

Hartmann-Goldstein

Koliantz

1981

Heredity

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SUMMARYThe effect of the Y chromosome on white variegation associated with In(1)w"4 has been investigated by comparing the occurrence, proportion and distribution of colourless cells in the Malpighian tubules of XO, XY and XYY larvae cultured at 25°C or 15°C. The results confirmed previous observations that the Y chromosome suppresses variegation, and showed that its loss from the XY complement has a much greater effect on the proportion of colourless cells than its addition. The variegation is markedly more affected by Y-chromosome constitution than by culture temperature. Colourless cells are non-randomly distributed within and between tubules; neither chromosome constitution nor temperature appears to modify these distribution patterns.

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Methods for Studying the Distribution of Pigment in Malpighian Tubules of Drosophila Melanogaster

Cited by 2 publications

References 7 publications

Functional domains are specified to single-cell resolution in a Drosophila epithelium

Functional domains are specified to single-cell resolution in a Drosophila epithelium

Modification of gene suppression in Drosophila melanogaster by sex chromosomes: I. Effect of the Y chromosome on variegation in malpighian tubules of wm4 larvae

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