Methods for Studying Oxidative Regulation of Protein Kinase C

Gopalakrishna, Rayudu; McNeill, Thomas H.; Elhiani, Albert A.; Gundimeda, Usha

doi:10.1016/b978-0-12-405881-1.00005-7

Cited by 6 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We discuss first the potential role of PKC/PLD on the plasma membrane remodeling by the H 2 O 2 stimulus. PKC belong to a family of enzymes with crucial role on many of the physiological and pathophysiological processes due to the H 2 O 2 -induced signaling [47]. The PKC amino acid sequence contains a group of redox cysteines that can be modulated by H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

LAURDAN fluorescence and phasor plots reveal the effects of a H2O2 bolus in NIH-3T3 fibroblast membranes dynamics and hydration

Malacrida

Gratton

2018

Free Radical Biology and Medicine

View full text Add to dashboard Cite

Fluorescence spectroscopy, coupled with microscopy, opens new frontiers for the study of dynamic processes with high spatio-temporal resolution. The application of phasor plots to FLIM and hyperspectral imaging demonstrate unprecedented capabilities to study complex photophysics at the subcellular level. Using these approaches we studied the effects of an HO bolus on NIH-3T3 membranes dynamics monitored by LAURDAN fluorescence. Exposure of NIH-3T3 cells to a bolus of HO modifies the cell membranes and, in particular, the plasma membrane in a complex manner. The LAURDAN results reveal that the peroxide treatment decreases membrane fluidity but surprisingly increases dipolar relaxation around the excited probe. Using the Multidimensional-phasor approach we elucidated the complex photophysics of LAURDAN incorporated into cell membrane after HO exposure. The results indicate the occurrence of LAURDAN fast-diffusion from gel↔l phases in membranes exposed to a HO bolus. An ad hoc hypothesis is presented to interpret the results in the context of HO oxidative distress/eustress.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Depending on its redox status, the enzyme could be either activated or inactivated. The in vitro threshold for activation is 1mM and below, but for concentration higher than 1mM PKC loses its activity [47]. The regulatory region of PKC is responsible for the enzyme activation due to oxidation of cysteines related to zinc-binding domain.…”

Section: Discussionmentioning

confidence: 99%

LAURDAN fluorescence and phasor plots reveal the effects of a H2O2 bolus in NIH-3T3 fibroblast membranes dynamics and hydration

Malacrida

Gratton

2018

Free Radical Biology and Medicine

View full text Add to dashboard Cite

show abstract

“…Must PKC isoforms, but mainly PKC-α and PKC-δ, have a singular structural characteristic, which makes them susceptible to oxidative modifications (Jin et al 2011;Lee et al 2012;Gopalakrishna et al 2013). PKC N-terminal presents regulatory domains that are bind to zinc and cysteine-rich motives, which are quickly oxidized by hydrogen peroxide.…”

Section: Parallel Mechanisms During Hormetic Oxidative Responsementioning

confidence: 99%

New considerations on hormetic response against oxidative stress

Luna–López

González-Puertos

López‐Díazguerrero

et al. 2014

J. Cell Commun. Signal.

View full text Add to dashboard Cite

In order to survive living organisms have developed multiple mechanisms to deal with tough environmental conditions. Hormesis is defined as a process in which exposure to a low dose of a chemical agent or environmental factor that is damaging at higher doses induces an adaptive beneficial effect on the cell or organism. In this paper, we examine several ideas that might be taken into consideration before using hormesis as a therapeutic tool to improve health and life span, and hopefully will open the discussion for new and interesting debates regard hormesis. The first one is to understand that the same stressor or inductor can activate different pathways in a parallel or dual response, which might lead to diverse outcomes. Another idea is related to the mechanisms involved in activating Nrf2, which might be different and have diverse hormetic effects.Last, we discuss mild oxidative stress in association to lowgrade chronic inflammation as a stimulating avenue to be explored and the unexpected effects proposed by the obesity paradox theory. All the previous might help to clarify the reasons why centenarians are able to reach the extreme limits of human life span, which could probably be related to the way they deal with homeostasis maintenance, providing an opportunity for hormesis to intervene significantly.

show abstract

“…Reactive oxygen species may stimulate a variety of targets, including AC, PKC, and phospholipase A2 (PLA2), which have been shown to play a role in the AR [90][91][92].…”

Section: Acrosome Reactionmentioning

confidence: 99%

Contemporary evidence on the physiological role of reactive oxygen species in human sperm function

Plessis

Agarwal

Halabi

et al. 2015

J Assist Reprod Genet

209

155

View full text Add to dashboard Cite

Reactive oxygen species (ROS) play an important role in male fertility. Overproduction of reactive oxygen species (ROS) has been associated with a variety of male fertility complications, including leukocytospermia, varicocele and idiopathic infertility. The subsequent oxidative insult to spermatozoa can manifest as insufficient energy metabolism, lipid peroxidation and DNA damage, leading to loss of motility and viability. However, various studies have demonstrated that physiological amounts of ROS play important roles in the processes of spermatozoa maturation, capacitation, hyperactivation and acrosome reaction. It is therefore crucial to define and understand the delicate oxidative balance in male reproductive cells and tissues for a better understanding of both positive as well as negative impact of ROS production on the fertilizing ability. This review will discuss the specific physiological roles, mechanisms of action and effects that ROS have on the acquisition of structural integrity and physiological activity of spermatozoa.

show abstract

Methods for Studying Oxidative Regulation of Protein Kinase C

Cited by 6 publications

References 36 publications

LAURDAN fluorescence and phasor plots reveal the effects of a H2O2 bolus in NIH-3T3 fibroblast membranes dynamics and hydration

LAURDAN fluorescence and phasor plots reveal the effects of a H2O2 bolus in NIH-3T3 fibroblast membranes dynamics and hydration

New considerations on hormetic response against oxidative stress

Contemporary evidence on the physiological role of reactive oxygen species in human sperm function

Contact Info

Product

Resources

About