Methods for Studying Bacterial Gene Transfer in Soil by Conjugation and Transduction

Stotzky, G.; Devanas, Monica A.; Zeph, Lawrence R.

doi:10.1016/s0065-2164(08)70243-0

Cited by 39 publications

(23 citation statements)

References 120 publications

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“…In the future more environmental compartments where gene transfer occurs will be identified. It is conceivable that Bräutigam et al, 1997;Davison, 1999;Day, 1998;Kay et al, 2002;Li et al, 2001;Lorenz and Wackernagel, 1994;Mercer et al, 2001;Schlimme et al, 1997;Stotzky et al, 1990. with improved experimental techniques gene transfer will be confirmed in very specific habitats where detection efforts have so far failed. For instance, it may be possible to identify natural transformation in the gastrointestinal tract, a habitat known to be rich in DNA degrading activity and probably not optimal for the development of DNA uptake competence of bacteria, but where high concentrations of bacteria are exposed to a variety of DNA molecules ingested with the food (Jonas et al, 2001).…”

Section: Microbial Horizontal Gene Transfer Processesmentioning

confidence: 99%

Microbial horizontal gene transfer and the DNA release from transgenic crop plants

Vries

Wackernagel

2005

Plant Soil

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Intraspecific and interspecific horizontal gene transfers among prokaryotes by mechanisms like conjugation, transduction and transformation are part of their life style. Experimental data and nucleotide sequence analyses show that these processes appear to occur in any prokaryotic habitat and have shaped microbial genomes throughout evolution over hundreds of million years. Here we summarize studies with a focus on the possibility of the transfer of free recombinant DNA released from transgenic plants to microorganisms by transformation. A list of 87 species capable of natural transformation is presented. We discuss monitoring techniques which allowed detection of the spread of intact DNA from plants during their growth, in the process of decay and by pollen dispersal including novel biomonitoring assays for measuring the transforming potential of DNA in the environment. Also, studies on the persistence of free DNA in soil habitats and the potential of bacteria to take up DNA in soil are summarized. On the other hand, the various barriers evolved in prokaryotes which suppress interspecific gene transfer and recombination will be addressed along with studies aiming to estimate the chance of a gene transfer from plant to microbe. The results suggest that, although such transfers could be possible in principle, each of the many steps involved from the release of intact DNA from a plant cell to integration into a prokaryotic genome has such a low probability that a successful transfer event be extremely rare. Further, interspecies transfer of chromosomal DNA is mostly negative for the recipient, and, if not, in the absence of a selective advantage the transformant will be lost. It is stressed that the nucleotide sequences introduced into transgenic plants are much less likely to be captured from the transgenic plants than directly from those organisms (often bacteria or viruses) from which they were originally derived.

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Section: Microbial Horizontal Gene Transfer Processesmentioning

confidence: 99%

Microbial horizontal gene transfer and the DNA release from transgenic crop plants

Vries

Wackernagel

2005

Plant Soil

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“…As a result of the ability to construct genetically modified organisms (GMOs), more studies are now examining the survival of and gene transfer by GMOs in soil and other natural habitats (Stotzky and Babich, 1986;Stotzky, 1989;Stotzky et al, 1990;. The results of such studies generally confirm those obtained with nonengineered microbes: the inoculated GMOs do not survive long or establish in soil, even if they are species that are indigenous to soil and even if the specific substrates are present on which the products of the novel genes function.…”

Section: Physical Chemical and Biological Soilmentioning

confidence: 99%

The International Workshop on Establishment of Microbial Inocula in Soils: Cooperative Research Project on Biological Resource Management of the Organization for Economic Cooperation and Development (OECD)

Elliott

Lynch

1995

Am J Alt Ag

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Low-input agriculture is likely to be the focal point for future cropping systems. Worldwide there is increasing concern that we must decrease the use of chemicals in agriculture. A leading reason for this concern has been the effects of pesticides on food quality, consumer health, and the environment. There also is concern regarding fertilizers and energy inputs because of environmental pollution from excessive application rates and poor timing of fertilization and because of the depletion of nonrenewable energy resources. Overuse of these materials not only is an economic waste but also may require environmental cleanup. Legislation may mandate the development of alternative methods of pest control. For example, the Dutch Government has demanded a 35% decrease in the use of farm pesticides in 1995 and a 50% reduction by the year 2000. Other governments may impose similar limitations.

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“…Several physicochemical and biological factors have been shown to influence the survival of GMMs introduced into the environment [2][3][4]. Results of studies are often contradictory, especially when experiments are conducted in a heterogeneous environment such as soil.…”

Section: Introductionmentioning

confidence: 99%

“…Although microbes are introduced in great numbers (log 8 to 9 cfu ml" 1 ) into soil, GMMs reaction to contact with natural soil is variable. Why do they undergo drastic elimination, though they are sometimes found even after long time periods [2,[5][6][7][8] ? It is interesting, whether additional genetic information introduced as plasmid can affect the efficiency of host survival in soil [2,9,10].…”

Section: Introductionmentioning

confidence: 99%

Survival, plasmid transfer and impact ofpseudomonas fluorescensintroduced into soil

Kozdrój

1997

Journal of Environmental Science and Health . Part A: Environme

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The survival of donor, recipient and transconjugant strains of Pseudomonas fluorescens, transfer of plasmid RP4 from donor to recipient, and impact of the introduced strains on indigenous microflora was determined in a sandy-loam soil. The introduced donor and recipient strains survived significantly better in sterile than in non-sterile soil where a progressive decline in their numbers with time was observed. In both soils, conjugation and plasmid RP4 transfer from donor to recipient cells was observed during the first 3 days. The transconjugant survived significantly better when it was the only strain inoculated into the soil. When introduced into soil pre-colonized by the recipient strain, the transconjugant was undetectable. The introduced strains caused stimulation of total number of indigenous heterotrophic bacteria and selected physiological groups such as proteolytic, amylolytic and am-1139

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Methods for Studying Bacterial Gene Transfer in Soil by Conjugation and Transduction

Cited by 39 publications

References 120 publications

Microbial horizontal gene transfer and the DNA release from transgenic crop plants

Microbial horizontal gene transfer and the DNA release from transgenic crop plants

The International Workshop on Establishment of Microbial Inocula in Soils: Cooperative Research Project on Biological Resource Management of the Organization for Economic Cooperation and Development (OECD)

Survival, plasmid transfer and impact ofpseudomonas fluorescensintroduced into soil

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