Methods for Studying and Structure–Function Relationships of Non-Fibrillar Protein Assemblies in Alzheimer's Disease and Related Disorders

Rahimi, Farid

doi:10.2174/9781608058525114020014

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2018

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“…Typical methods for analysis of the aggregation reaction, con-formational change, and morphology of the protein assemblies include thioflavin T fluorescence, dynamic light scattering, circular dichroism spectroscopy, and electron microscopy [40, 41] ( see Note 6 in Subheading 4.1). …”

Section: Methodsmentioning

confidence: 99%

Using Molecular Tweezers to Remodel Abnormal Protein Self-Assembly and Inhibit the Toxicity of Amyloidogenic Proteins

Malik

Nair

et al. 2018

Methods in Molecular Biology

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Molecular tweezers (MTs) are broad-spectrum inhibitors of abnormal protein self-assembly, which act by binding selectively to lysine and arginine residues. Through this unique mechanism of action, MTs inhibit formation of toxic oligomers and aggregates. Their efficacy and safety have been demonstrated in vitro, in cell culture, and in animal models. Here, we discuss the application of MTs in diverse in vitro and in vivo systems, the experimental details, the scope of their use, and the limitations of the approach. We also consider methods for administration of MTs in animal models to measure efficacy, pharmacokinetic, and pharmacodynamic parameters in proteinopathies.

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Section: Methodsmentioning

confidence: 99%