Methods for sampling and analysis of the aqueous phase of human dental plaque

Tatevossian, A.; Gould, Catherine

doi:10.1016/0003-9969(76)90054-6

Cited by 25 publications

(13 citation statements)

References 15 publications

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“…21 One hour after sucrose rinses the plaque fluid in the caries-positive individuals fell to 5.88 AE 0.37. 21 Other authors have studied plaque fluid pH and obtained values of 5.73 AE 0.22, 22 6.54 AE 0.30, 23 5.73 AE 0.79 24 and 6.76 AE 0.04. 25 The surface pH of sound enamel and WSEL reported in this study are similar to the plaque fluid measurements reported by Margolis and Moreno 21 and within the range reported by the other authors.…”

Section: Discussionmentioning

confidence: 97%

“…25 The surface pH of sound enamel and WSEL reported in this study are similar to the plaque fluid measurements reported by Margolis and Moreno 21 and within the range reported by the other authors. [22][23][24][25] Therefore, the surface pH measurements may reflect the pH of that environment prior to plaque removal and may relate to the pH of the enamel fluid.…”

Section: Discussionmentioning

confidence: 99%

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The clinical application of surface pH measurements to longitudinally assess white spot enamel lesions

Kitasako

Cochrane

Matin

et al. 2010

Journal of Dentistry

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

The clinical application of surface pH measurements to longitudinally assess white spot enamel lesions

Kitasako

Cochrane

Matin

et al. 2010

Journal of Dentistry

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“…Assuming a calcium ion activity product in plaque fluid of 1-2 mM [Tatevossian and Gould, 1976;Vogel et al, 2000], it can easily be calculated that a fluoride concentration of only a few ppm is required to fulfill the solubility product of calcium fluoride [Øgaard, 1985]. Surface adsorption of fluoride to crystal surfaces may cause local higher concentrations of fluoride than in plaque fluid.…”

Section: Calcium Fluoride Formationmentioning

confidence: 99%

CaF<sub>2</sub> Formation: Cariostatic Properties and Factors of Enhancing the Effect

Øgaard¹

2001

Caries Res

109

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CaF₂ or a CaF₂-like material/phosphate-contaminated CaF₂ is a major reaction product during topical treatment of dental hard tissues. Recently, evidence has suggested that CaF₂ is formed not only on surfaces but also to some extent in the enamel. The minimum concentration of fluoride required for CaF₂ formation is not well known and may depend on whether calcium is available from plaque fluid or only through dissolution of the dental hard tissue. Furthermore, surface adsorption of fluoride to crystals may cause local concentrations necessary for CaF₂ formation. It has been suggested that CaF₂ acts as a pH-controlled reservoir of fluoride. The rate-controlling factor appears to be phosphate, which controls the dissolution rate of CaF₂ at high pH. Increasing fluoride concentration, prolonging the exposure time or using a fluoride solution with low pH can increase CaF₂ formation. CaF₂ formed at low pH contains less internal phosphate which has been shown to be less soluble. This may be of clinical significance for fluoride applied topically a few times per year.

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“…To illustrate this point, devices were removed from the volunteers and immediately transported to the laboratory in a hu midified collecting vessel (time § 5 min). The device, with the plaque undisturbed within, was then exposed to solutions, approximating to the inorganic composition of saliva [Tatevossian and Gould, 1976], containing either 5 pCi/ml l4C-sucrose or 5 pCi/ntl ,2P-phosphate in 60 mM Tris, 130 mM KCI, 1.5 mM Ca(NO,):4 H :0. 3m M KH: P 0 4, pH 71 for 30 s. The device was then briefly dipped into a wash solution containing the buffer only, carefully blotted with paper tissue and snap frozen as described above.…”

Section: Determination O F Penetration O F 14 C-sucrose and 32 P-phosmentioning

confidence: 99%

A Method for the Quantitative Site-Specific Study of the Biochemistry within Dental Plaque Biofilms Formed in vivo

Robinson¹,

Kirkham²,

Percival³

et al. 1997

Caries Res

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The study of plaque biofilms in the oral cavity is difficult as plaque removal inevitably disrupts biofilm integrity precluding kinetic studies involving the penetration of components and metabolism of substrates in situ. A method is described here in which plaque is formed in vivo under normal (or experimental) conditions using a collection device which can be removed from the mouth after a specified time without physical disturbance to the plaque biofilm, permitting site-specific analysis or exposure of the undisturbed plaque to experimental conitions in vitro. Microbiological analysis revealed plaque flora which was similar to that reported from many natural sources. Analytical data can be related to plaque volume rather than weight. Using this device, plaque fluoride concentrations have been shown to vary with plaque depth and in vitro short-term exposure to radiolabelled components may be carried out, permitting important conclusions to be drawn regarding the site-specific composition and dynamics of dental plaque.

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Methods for sampling and analysis of the aqueous phase of human dental plaque

Cited by 25 publications

References 15 publications

The clinical application of surface pH measurements to longitudinally assess white spot enamel lesions

The clinical application of surface pH measurements to longitudinally assess white spot enamel lesions

CaF<sub>2</sub> Formation: Cariostatic Properties and Factors of Enhancing the Effect

A Method for the Quantitative Site-Specific Study of the Biochemistry within Dental Plaque Biofilms Formed in vivo

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