Methods for Replicating Leaf Vibrations Induced by Insect Herbivores

Michael, Sabrina C. J.; Appel, Heidi M.; Cocroft, Reginald B.

doi:10.1007/978-1-4939-9458-8_15

Cited by 5 publications

(2 citation statements)

References 20 publications

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“…Feeding vibrations of P. rapae caterpillar (Lepidoptera, Pieridae) recorded by Appel and Cocroft (2014) (Figure 1A–C) were played back to plants for 24 h ( N = 12 sets of three plants for phytohormones and 24 for VOCs). Playbacks were conducted using 2-inch audio speakers (8 ohms, 0.5 W), modified as in Michael et al (2019) to minimize production of airborne sound, and with a dowel attached to the coil to allow coupling of vibrations to a leaf (Figure 1F). The speakers were driven using stereo amplifiers (Dayton Audio, DTA1).…”

Section: Methodsmentioning

confidence: 99%

Caterpillar Chewing Vibrations Cause Changes in Plant Hormones and Volatile Emissions in Arabidopsis thaliana

et al. 2019

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Plant perception of insect feeding involves integration of the multiple signals involved: wounding, oral secretions, and substrate borne feeding vibrations. Although plant responses to wounding and oral secretions have been studied, little is known about how signals from the rapidly transmitted vibrations caused by chewing insect feeding are integrated to produce effects on plant defenses. In this study, we examined whether 24 h of insect feeding vibrations caused changes in levels of phytohormones and volatile organic compounds (VOCs) produced by leaves of Arabidopsis thaliana when they were subjected to just feeding vibrations or feeding vibrations and wounding + methyl jasmonate (MeJA), compared to their respective controls of silent sham or wounding + MeJA. We showed that feeding vibrations alone caused a decrease in the concentrations of most phytohormones, compared to those found in control plants receiving no vibrations. When feeding vibrations were combined with wounding and application of MeJA, the results were more complex. For hormones whose levels were induced by wounding and MeJA (jasmonic acid, indole-3-butyric acid), the addition of feeding vibrations caused an even larger response. If the level of hormone was unchanged by wounding and MeJA compared with controls, then the addition of feeding vibrations had little effect. The levels of some VOCs were influenced by the treatments. Feeding vibrations alone caused an increase in β-ionone and decrease in methyl salicylate, and wounding + MeJA alone caused a decrease in benzaldehyde and methyl salicylate. When feeding vibrations were combined with wounding + MeJA, the effects on β-ionone and methyl salicylate were similar to those seen with feeding vibrations alone, and levels of benzaldehyde remained low as seen with wounding + MeJA alone. The widespread downregulation of plant hormones observed in this study is also seen in plant responses to cold, suggesting that membrane fluidity changes and/or downstream signaling may be common to both phenomena.

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Section: Methodsmentioning

confidence: 99%

Caterpillar Chewing Vibrations Cause Changes in Plant Hormones and Volatile Emissions in Arabidopsis thaliana

et al. 2019

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“…Vibrations produced by fourth instar P. rapae caterpillars feeding on leaves of potted A. thaliana were recorded using a laser vibrometer (Appel and Cocroft, 2014). Feeding vibrations were played back to A. thaliana plants for 2 h using piezoelectric stack actuators as in Appel and Cocroft (2014), with the frequency and amplitude characteristics of the playback exemplars closely matching those of the original recordings (see Michael et al, 2019 for detailed methods).…”

Section: Methodsmentioning

confidence: 99%

Use of Yellow Fluorescent Protein Fluorescence to Track OPR3 Expression in Arabidopsis Thaliana Responses to Insect Herbivory

et al. 2019

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Feeding by chewing insects induces chemical defenses in plants that are regulated by the jasmonic acid (JA) pathway. Jasmonates are usually quantified by liquid chromatography–mass spectrometry (LC-MS) analysis of precursors and products in the biosynthetic pathway or inferred from the extraction and expression of genes known to respond to elevated levels of JA. Both approaches are costly and time consuming. To address these limitations, we developed a rapid reporter for the synthesis of JA based on the OPR3promoter:YFP-PTS1. Yellow fluorescent protein (YFP) fluorescence was increased by mechanical wounding and methyl jasmonate (MeJA) treatment and by caterpillar feeding. To develop an optimal sampling time for a quantitative bioassay, OPR3promoter:YFP-PTS1 plants were sampled at 1, 2, 3, and 24 h after treatment with 115 µM MeJA. The first increase in YFP fluorescence was detected at 2 h and remained elevated 3 and 24 h later; as a result, 3 h was chosen as the sampling time for a quantitative bioassay of jasmonate response to insect attack. Feeding by Pieris rapae caterpillars induced a 1.8-fold increase in YFP fluorescence, consistent with the known induction of JA production by this insect. We also assessed the utility of this reporter in studies of plant responses to caterpillar feeding vibrations, which are known to potentiate the JA-dependent production of chemical defenses. Pretreatment with feeding vibrations increased expression of the OPR3promoter:YFP-PTS1 in response to 14 µM MeJA. Feeding vibrations did not potentiate responses at higher MeJA concentrations, suggesting that potentiating effects of prior treatments can only be detected when plants are below a response threshold to the elicitor. The expression of OPR3 does not indicate levels of specific downstream jasmonates and quantification of specific jasmonates still requires detailed analysis by LC-MS. However, OPR3 expression does provide a rapid and inexpensive way to screen large numbers of plants for the involvement of jasmonate signaling in their response to a wide variety of treatments, and to study the induction and expression of AtOPR3.

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Blooms and Buzzing Bees: Bridging Buzz Pollination and Biotremology

Luca

Vallejo‐Marín

2022

Biotremology: Physiology, Ecology, and Evolution

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Methods for Replicating Leaf Vibrations Induced by Insect Herbivores

Cited by 5 publications

References 20 publications

Caterpillar Chewing Vibrations Cause Changes in Plant Hormones and Volatile Emissions in Arabidopsis thaliana

Caterpillar Chewing Vibrations Cause Changes in Plant Hormones and Volatile Emissions in Arabidopsis thaliana

Use of Yellow Fluorescent Protein Fluorescence to Track OPR3 Expression in Arabidopsis Thaliana Responses to Insect Herbivory

Blooms and Buzzing Bees: Bridging Buzz Pollination and Biotremology

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