Methods for quantitative analysis of PGF2α, PGE2, 9α,11α-dihydroxy-15-keto-prost-5-enoic acid and 9α,11α,15-trihydroxy-prost-5-enoic acid from body fluids using deuterated carriers and gas chromatography-mass spectrometry

Gréen, K.; Granström, Elisabeth; Samuelsson, Bengt; Axen, Udo

doi:10.1016/0003-2697(73)90373-4

Cited by 110 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One min after the addition of thrombin, the clumped platelets were removed by filtration under reduced pressure and samples of 1 ml of the filtrate were removed and added (2.5-3.5 min after addition of thrombin) to 15 ml of ethanol and to 15 ml of ethanol containing 75 mg of SnCl2. PGF2a was determined in these solutions by multiple-ion analysis with [3,3,4,4-2H4]PGF2, as carrier (9). The amount in the nonreduced platelet medium was 11.7 + 1.9 ng/ml (three experiments) and in the reduced medium 30.2 ± 6.3 ng/ml (three experiments), indicating the presence of PGG2 and/or PGH2.…”

Section: E25mentioning

confidence: 99%

Isolation and Structure of Two Prostaglandin Endoperoxides That Cause Platelet Aggregation

Hámberg

Svensson

Wakabayashi

et al. 1974

Proc. Natl. Acad. Sci. U.S.A.

848

290

View full text Add to dashboard Cite

Incubation for a short time of arachidonic acid with the microsomal fraction of a homogenate of the vesicular gland of sheep in the presence of 1 mM p-mercuribenzoate followed by extraction and silicic acid chromatography yielded two prostaglandin endoperoxides. The structures of these compounds, i.e., 15-hydroperoxy-9a,11a-peroxidoprosta-5,13-dienoic acid (prostaglandin G2) and 15-hydroxy-9a,lla-peroxidoprosta-5,13-dienoic acid (prostaglandin H2), were assigned mainly by a number of chemical transformations into previously known prostaglandins. The new prostaglandins were 50-200 times (prostaglandin G2) and 100-450 times (prostaglandin H2) more active than prostaglandin E2 on the superfused aorta strip. The half-life of the prostaglandin endoperoxides in aqueous medium (about 5 min) was significantly longer than that of "rabbit aorta-contracting substance" released from guinea pig lung, indicating that none of the prostaglandin endoperoxides is identical with this factor. Addition of 10-300 ng/ml of the endoperoxides to suspensions of washed human platelets resulted in rapid aggregation. Furthermore, platelet aggregation induced by thrombin was accompanied by release of material reducible by stannous chloride into prostaglandin F2a, thus indicating the involvement of endogenous prostaglandin endoperoxides in platelet aggregation.

show abstract

Section: E25mentioning

confidence: 99%

Isolation and Structure of Two Prostaglandin Endoperoxides That Cause Platelet Aggregation

Hámberg

Svensson

Wakabayashi

et al. 1974

Proc. Natl. Acad. Sci. U.S.A.

848

290

View full text Add to dashboard Cite

show abstract

“…Clearly, measurement of PG in urine can be accomplished more readily than in plasma where platelets generate PG rapidly (27,32) and may be an important factor in causing the large discrepancies in reported plasma levels (28,33). Also, renal venous blood sampling is limited to the animal model or to the acute study in man.…”

Section: Human Studiesmentioning

confidence: 99%

Urinary prostaglandins. Identification and origin.

Frölich¹,

Wilson²,

Sweetman³

et al. 1975

J. Clin. Invest.

497

136

View full text Add to dashboard Cite

A BSTRA CT Human urine was analyzed by mass spectrometry for the presence of prostaglandins. Pros-taglandin E2 and F2. were detected in urine from females by selected ion monitoring of the prostaglandin E2-methylester-methoxime bis-acetate and the prostaglandin F2a-methyl ester-Tris-trimethylsilylether de-rivative. Additional evidence for the presence of prostaglandin F2. was obtained by isolating from female urine an amount of this prostaglandin sufficient to yield a complete mass spectrum. The methods utilized permitted quantitative analysis.The origin of urinary prostaglandin was determined by stimulating renal prostaglandin synthesis by arachidonic acid or angiotensin infusion. Arachidonic acid, the precursor of prostaglandin E2, when infused into one renal artery of a dog led to a significant increase in the excretion rate of this prostaglandin. Similarly, infusion of angiotensin II amide led to a significantly increased ipsilateral excretion rate of prostaglandin E2 and F2. in spite of a simultaneous decrease in the creatinine clearance. In man, i.v. infusion of angiotensin also led to an increased urinary elimination of prostaglandin E.These results show that urinary prostaglandins may originate from the kidney, indicating that renally synthesized prostaglandins diffuse or are excreted into the tubule. Thus, urinary prostaglandins are a reflection of renal prostaglandin synthesis and have potential as a tool to delineate renal prostaglandin physiology and pathology.

show abstract

“…Quantitative Analyses of Prostaglandin E2, Prostaglandin F2, and Arachidonic Acid These analyses were performed by quantitative mass spectrometry as described previously for prostaglandins E2 and F l a [18] and for arachidonic acid [19]. Results of prostaglandin analyses were expressed as ng/ml culture medium (cf.…”

Section: Cell Culturesmentioning

confidence: 99%

Prostaglandin Production by Normal and Transformed 3T3 Fibroblasts in Cell Culture

Hammarström

1977

European Journal of Biochemistry

View full text Add to dashboard Cite

Prostaglandin EZ and prostaglandin Fza were quantitatively determined in culture media from Balb/c 3T3 fibroblasts and two virus-transformed derivatives of these cells. Regular and (simian virus 40)-transformed 3T3 cells produced low and almost identical prostaglandin EZ concentrations. The levels were maximal (10 ng/ml) 24 h after planting and decreased during the next 96 h to 2.5 ng/ml. In contrast, polyoma-virus-transformed 3T3 cells produced prostaglandin E2 continuously for 120 h, giving a final concentration of 270 ng/ml.Fresh medium supplemented with calf serum reinitiated prostaglandin production in confluent 3T3 cells. Maximal prostaglandin E2 concentrations were obtained between 6 and 18 h after medium change and were proportional to the serum concentration of the medium. Indomethacin M) completely inhibited prostaglandin E2 production but not prostaglandin F2a production by these cells.E-type prostaglandins are potent stimulators of adenylate cyclase in a number of tissues [I]. On the other hand, prostaglandin Flcr raised the level of cyclic GMP in vein strips [2]. Since cyclic AMP and cyclic GMP appear to have important roles in the regulation of cell proliferation and differentiation [3 -111 it seemed essential to measure endogenous prostaglandin production by cultured cells and to determine what effects this might have on cyclic nucleotide metabolism. We have previously reported that baby hamster kidney (BHK) fibroblasts release prostaglandins E2 and Fza into the culture medium and that polyomavirus transformation of these cells raises their prostaglandin production [12]. In this report a similar observation was made regarding the effect of polyomavirus transformation on prostaglandin production by 3T3 fibroblasts. In contrast, transformation of 3T3 cells by simian virus 40 had no appreciable effect on the production of prostaglandins E2 and Fz,. A Trivial Names and Abbreviations. Prostaglandin El, 1 lci,lS(S)-dihydroxy-9-ketoprosta-l3(truns)-enoic acid; prostaglandin € 2 , 11~,15(S)-dihydroxy-9-ketoprosta-5(cis),l3(trans)-dienoic acid, prostaglandin Fzr, 9a,l Ia,l5(S)-trihydroxyprosta-5(cis),l3(tramjdienoic acid ; py 3T3 cells, a polyoma-virus-transformed 3T3 mouse fibroblast line; SV 3T3 cells, a (simian virus 40)-transformed 3T3 mouse fibroblast line; py BHK cells, a polyoma-virus-transformed baby hamster kidney fibroblast line; indomethacin, 1-@-chlorobenzoyl)-5-methoxy-2-methylindole-3-acetic acid; cyclic AMP, adenosine 3': 5'-monophosphate; cyclic GMP, guanosine 3' : 5'-monophosphate. subsequent paper will describe effects of the elevated prostaglandin E2 production by polyoma-virus-transformed 3T3 cells on cellular levels of cyclic AMP [13]. EZ and [3,3,4, MATERIALS AND METHODS [3,3,4,4-2H4]Prostaglandin Cell CulturesBalb/c 3T3 mouse embryo fibroblasts, polyomavirus-transformed (py 3T3) and (simian virus 40)-transformed derivatives of these cells

show abstract

Methods for quantitative analysis of PGF2α, PGE2, 9α,11α-dihydroxy-15-keto-prost-5-enoic acid and 9α,11α,15-trihydroxy-prost-5-enoic acid from body fluids using deuterated carriers and gas chromatography-mass spectrometry

Cited by 110 publications

References 21 publications

Isolation and Structure of Two Prostaglandin Endoperoxides That Cause Platelet Aggregation

Isolation and Structure of Two Prostaglandin Endoperoxides That Cause Platelet Aggregation

Urinary prostaglandins. Identification and origin.

Prostaglandin Production by Normal and Transformed 3T3 Fibroblasts in Cell Culture

Contact Info

Product

Resources

About