Methods for quantifying lysophosphatidic acid in body fluids: A review

Jesionowska, Anna; Cecerska, Elżbieta; Dołęgowska, Barbara

doi:10.1016/j.ab.2014.02.021

Cited by 29 publications

(23 citation statements)

References 63 publications

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“…There are many methods for LPA quantification (for review see [28]). Some of them allow quantification of LPA molecular species and other only total LPA concentration in the samples.…”

Section: Discussionmentioning

confidence: 99%

Lysophosphatidic acid plasma concentrations in healthy subjects: circadian rhythm and associations with demographic, anthropometric and biochemical parameters

et al. 2017

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BackgroundLysophosphatidic acid (LPA) is a bioactive lipid with a wide biological activity. Previous studies have shown its potential usefulness as a diagnostic marker for ovarian cancer. The aim of the study was to investigate which factors may influence plasma LPA concentrations in healthy subjects and to propose reference values.MethodsThe study group consisted of 100 healthy subjects. From all of them the blood samples were taken at 7 a.m. (fasting state). From 40 volunteers additional blood samples were taken at 2 p.m., at 8 p.m. and at 2 a.m. next morning. Concentrations of LPA were measured in plasma samples using enzyme-linked immunosorbent assay.ResultsAnalysis of samples from 100 healthy volunteers showed significant influence of sex and age on plasma LPA. The reference range for the plasma LPA concentration corrected for age and sex, determined at 2.5–97.5 percentile interval is 0.14–1.64 μM. LPA correlates positively with BMI, serum total cholesterol, triacylglycerols, uric acid and negatively with estimated glomerular filtration rate and serum albumin. Concentration of LPA at 2 a.m. was lower than at 2 p.m. There were not any significant differences between plasma LPA at 7 a.m. and any other time of the day.ConclusionsPlasma LPA is associated with demographic, anthropometric and biochemical parameters. It seems that LPA concentrations have no specific circadian rhythm and the time of donation and fasting state have marginal effect on plasma LPA. These findings may be helpful in future incorporation of LPA as a diagnostic marker.

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“…There are many methods for LPA quantification (for review see [28]). Some of them allow quantification of LPA molecular species and other only total LPA concentration in the samples.…”

Section: Discussionmentioning

confidence: 99%

Lysophosphatidic acid plasma concentrations in healthy subjects: circadian rhythm and associations with demographic, anthropometric and biochemical parameters

et al. 2017

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“…PtdOH constitutes between 2 and 6% in the cellular membranes and that accounts for about 10% of the PC content (typically 30-50% of the total glycerophospholipids, depending on the biologic source) of the membranes (Andreyev et al, 2010;Shulga et al, 2013). By comparison, LPA is typically found to be less than 1% (nanomolar quantities) (Sugiura et al, 1999;Jesionowska et al, 2014), whereas total PIP n represents less than 1% of the membrane phospholipids (Czech, 2000). The amount of PtdOH in basal conditions depends on the cell, tissue, or organ where it is measured, and it may double under certain types of stimulation.…”

Section: Functions Of Phosphatidic Acidmentioning

confidence: 99%

Phospholipase D Signaling Pathways and Phosphatidic Acid as Therapeutic Targets in Cancer

2014

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“…LPA is also found in many biological fluids, including plasma, serum, saliva, tears, aqueous humour, follicular fluid and cerebrospinal fluid at biologically meaningful concentrations (Aoki, 2004;Yung et al, 2014), and is usually bound to carrier proteins such as albumin. The total or specific forms of LPA present in a sample can be identified and quantified via colorimetric assays, mass spectrometry and other methods (Jesionowska et al, 2014).…”

Section: Lpa Production and Metabolismmentioning

confidence: 99%

Lysophosphatidic acid signalling in development

et al. 2015

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Lysophosphatidic acid (LPA) is a bioactive phospholipid that is present in all tissues examined to date. LPA signals extracellularly via cognate G protein-coupled receptors to mediate cellular processes such as survival, proliferation, differentiation, migration, adhesion and morphology. These LPA-influenced processes impact many aspects of organismal development. In particular, LPA signalling has been shown to affect fertility and reproduction, formation of the nervous system, and development of the vasculature. Here and in the accompanying poster, we review the developmentally related features of LPA signalling.

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Methods for quantifying lysophosphatidic acid in body fluids: A review

Cited by 29 publications

References 63 publications

Lysophosphatidic acid plasma concentrations in healthy subjects: circadian rhythm and associations with demographic, anthropometric and biochemical parameters

Lysophosphatidic acid plasma concentrations in healthy subjects: circadian rhythm and associations with demographic, anthropometric and biochemical parameters

Phospholipase D Signaling Pathways and Phosphatidic Acid as Therapeutic Targets in Cancer

Lysophosphatidic acid signalling in development

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