Methods for Purification and Characterization of Microbial Phenazines

Abstract

Phenazines are nitro aromatic redox-active antibiotics produced under the control of quorum sensing mechanism by a diverse range of bacterial genera with various color intensities varies from blue, green, purple, yellow, red to even brown. Production of phenazine showed (1) taxonomic value when related to few genera, (2) functional role in environment and (3) bioactive potential with respect to structure; however due to poor structural understanding its correlation is still incomplete with diversity and bioactivity. Thus isolation and identification of phenazine antibiotic is highly desirable. Current chapter resolves the methodologies for various phenazines isolation as well as its identification through different spectroscopic and electrophoretic methods in different bacterial systems. Abbreviations MS Mass spectrometry EI-MSElectron impact mass spectroscopy HRMS High-resolution mass spectrometry FI MS Field ionization mass spectrometry HRESIMS High resolution electron spray ionization mass spectroscopy LRCIMS Low resolution chemical ionization mass spectrometry HRCIMS High resolution chemical ionization mass spectrometry HR-FAB-MS High resolution fast atom bombardment mass spectrum DCI-MS Desorption-chemical ionization mass spectrometry LC/MS

Streptomyces sp. CLV45 from Fabaceae rhizosphere benefits growth of soybean plants

“…Phenazines were identified and quantified using Streptomyces isolates grown in 10 mL ISP2 liquid medium at 100 rpm and 26 ± 2°C for 7 days (decline phase). Extraction and characterization procedures followed that of Kadam et al [25] and Cezairliyan et al [26], with modifications. For extraction of phenazines, 20 mL of each isolate suspension was centrifuged, and the supernatant was divided equally into two flasks.…”