Methods for Preparing Tissue Culture in Disposable Microplates and Their Use in Virology1

Sullivan, Elizabeth J.; Rosenbaum, Max J.

doi:10.1093/oxfordjournals.aje.a120704

Cited by 33 publications

(15 citation statements)

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“…Titrations of established antibody levels were deter mined for four viruses. Poliovirus type I and herpes simplex antibodies were measured by the standard microneutralization assay using H.Ep-2 culture [22], Cytomegalovirus anti body was measured by the microtiter complement fixation test. EB virus antibody was measured by the indirect fluorescent antibody technique using acetone fixed EB-3 cells as antigen [11].…”

Section: Methodsmentioning

confidence: 99%

Immunocompetence in Advanced Cancer Patients prior to Chemotherapy

Lehane¹,

Lane

1974

Oncology

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A panel of immunologic tests were used to characterize the levels of immunocompetence in 30 patients with advanced lung and head and neck region cancer prior to their receiving chemotherapy. All cell-mediated immunity parameters tested were significantly depressed in these patients when compared to controls. Antibody-mediated immunity tests showed lower levels of isoantibody titers to blood group antigens, no difference from controls in titrations for established virus antibodies and significantly elevated serum IgA and complement levels. Primary vaccination with KLH revealed marked deficiencies in both the antibody and cell-mediated immune response. The degree of immunoincom-petence did not correlate with either response to chemotherapy or the diagnosis to study interval which emphasizes the severity of immunoincompetence in patients with advanced stages of disease.

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Section: Methodsmentioning

confidence: 99%

Immunocompetence in Advanced Cancer Patients prior to Chemotherapy

Lehane¹,

Lane

1974

Oncology

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“…The CEK cells were prepared as described by Sullivan and Rosenbaum (1967) with some modifications. Briefly, the 17-20-day-old embryonating eggs were candled and the edge of the air sac was marked.…”

Section: Preparation Of Cek Cellsmentioning

confidence: 99%

“…Cell culture systems were developed for the titration of human or animal viruses that will replicate in vitro (Sullivan and Rosenbaum, 1967). The presence of infective virus is most conveniently indicated by the production of cytopathic effect (CPE).…”

Section: Introductionmentioning

confidence: 99%

Development of a Cell Culture Method for Quantal Assay of Strain I-2 of Newcastle Disease Virus

2006

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Repeated titrations of strains of Newcastle disease virus (NDV) are more conveniently undertaken in cell cultures rather than in embryonated eggs. This is relatively easy with mesogenic and velogenic strains that are cytopathic to various cell lines, but is difficult with avirulent Australian isolates that are poorly cytopathic. Strain V4 for example has been shown to be pathogenic iin vitro only to of chicken embryo liver cells. Strain I-2 was reported to produce cytopathic effect (CPE) on chicken embryo kidney (CEK) cells. The present studies confirmed this observation and developed a quantal assay. CEK cells infected with strain I-2 developed CPE characterized by degeneration, rounding, granularity and vacuolation, and the formation of synctia. End points were readily established by microscopic examination of fixed and stained cells. In virus infectivity studies on strain I-2, where multiple titrations are required and where large numbers of samples are used, titration using CEK cell grown in microtitre plates is recommended. Such studies may not be feasible in embryonated eggs.

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“…As provas de neutralização foram realizadas segundo à microtécnica 18,21 . Os soros foram examinados na diluição de 1 : 8.…”

Section: Métodos De Laboratóriounclassified

Nível de imunidade contra a poliomielite em um grupo de crianças vacinadas de acordo com o calendário oficial de imunização (São Paulo, Brasil)

et al. 1978

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Methods for Preparing Tissue Culture in Disposable Microplates and Their Use in Virology1

Cited by 33 publications

References 0 publications

Immunocompetence in Advanced Cancer Patients prior to Chemotherapy

Immunocompetence in Advanced Cancer Patients prior to Chemotherapy

Development of a Cell Culture Method for Quantal Assay of Strain I-2 of Newcastle Disease Virus

Nível de imunidade contra a poliomielite em um grupo de crianças vacinadas de acordo com o calendário oficial de imunização (São Paulo, Brasil)

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