Methods for nano-particle based vaccine formulation and evaluation of their immunogenicity

Kalkanidis, Martha; Pietersz, Geoffrey A.; Xiang, Sue D.; Mottram, Patricia L.; Crimeen‐Irwin, Blessing; Ardipradja, Katie; Plebanski, Magdalena

doi:10.1016/j.ymeth.2006.05.018

Cited by 89 publications

(70 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, effective vaccines to eliminate pathogens entrenched in the human population, such as malaria, leishmaniasis, measles, acute respiratory infections, and HIV, are still to be developed (Kalkanidis et al 2006;Nagill and Kaur 2010). Traditional vaccines consist of either whole-inactivated or liveattenuated microorganisms.…”

Section: New Perspectives For Applications Of Proteoliposomesmentioning

confidence: 99%

Proteoliposomes in nanobiotechnology

et al. 2012

View full text Add to dashboard Cite

Proteoliposomes are systems that mimic lipid membranes (liposomes) to which a protein has been incorporated or inserted. During the last decade, these systems have gained prominence as tools for biophysical studies on lipid-protein interactions as well as for their biotechnological applications. Proteoliposomes have a major advantage when compared with natural membrane systems, since they can be obtained with a smaller number of lipidic (and protein) components, facilitating the design and interpretation of certain experiments. However, they have the disadvantage of requiring methodological standardization for incorporation of each specific protein, and the need to verify that the reconstitution procedure has yielded the correct orientation of the protein in the proteoliposome system with recovery of its functional activity. In this review, we chose two proteins under study in our laboratory to exemplify the steps necessary for the standardization of the reconstitution of membrane proteins in liposome systems: (1) alkaline phosphatase, a protein with a glycosylphosphatidylinositol anchor, and (2) Na,K-ATPase, an integral membrane protein. In these examples, we focus on the production of the specific proteoliposomes, as well as on their biochemical and biophysical characterization, with emphasis on studies of lipid-protein interactions. We conclude the chapter by highlighting current prospects of this technology for biotechnological applications, including the construction of nanosensors and of a multiprotein nanovesicular biomimetic to study the processes of initiation of skeletal mineralization.

show abstract

Section: New Perspectives For Applications Of Proteoliposomesmentioning

confidence: 99%

Proteoliposomes in nanobiotechnology

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Additionally, the siRNA nanoplex should have minimal effects on nontarget genes and should avoid inadvertent stimulation of the immune system. 77,78 Delivery is a key determinant as to whether or not RNA interferencebased therapeutics will have clinical relevance and delivery encompasses extracellular transport of the nanoplex to target cells, its intracellular RNA trafficking, and processing. [79][80][81][82][83][84][85][86][87][88][89] Gold nanoparticles are particularly attractive for therapeutic applications due to their biocompatibility and ease of complex formation with biomolecules.…”

Section: 7576mentioning

confidence: 99%

Anti-HIV-1 nanotherapeutics: promises and challenges for the future

Mahajan¹,

Aalinkeel²,

Law³

et al. 2012

IJN

121

View full text Add to dashboard Cite

Abstract:The advent of highly active antiretroviral therapy (HAART) has significantly improved the prognosis for human immunodeficiency virus (HIV)-infected patients, however the adverse side effects associated with prolonged HAART therapy use continue. Although systemic viral load can be undetectable, the virus remains sequestered in anatomically privileged sites within the body. Nanotechnology-based delivery systems are being developed to target the virus within different tissue compartments and are being evaluated for their safety and efficacy. The current review outlines the various nanomaterials that are becoming increasingly used in biomedical applications by virtue of their robustness, safety, multimodality, and multifunctionality. Nanotechnology can revolutionize the field of HIV medicine by not only improving diagnosis, but also by improving delivery of antiretrovirals to targeted regions in the body and by significantly enhancing the efficacy of the currently available antiretroviral medications.

show abstract

“…Furthermore, nanoparticles can also be designed to provoke an immune response, by either direct immunostimulation of antigen presenting cells or delivering antigens to specific cellular compartments [64].…”

Section: Biodegradable Polymeric Nanoparticlesmentioning

confidence: 99%

“…It is considered that by using these particles, the antigen can be presented to the immune system for extended periods of time, and thus improve the immunogenicity, probably due to the persistence of the nanoparticles in the tissues [70,77]. On the other hand, these polymers have several technological advantages [64]. For example, polystyrene nanoparticles can be made with several functional groups on their surface to achieve effective conjugation with a variety of antigens.…”

Section: Nondegradable Polymeric Nanoparticlesmentioning

confidence: 99%

Genetic toxicology and preliminaryin vivostudies of nitric oxide donor tocopherol analogs as potential new class of antiatherogenic agents

Cabrera

López

Gómez

et al. 2011

Drug and Chemical Toxicology

View full text Add to dashboard Cite

In the last decades, significant progress in research and clinics has been made to offer possible innovative therapeutics for the management of allergic diseases. However, current allergen immunotherapy shows limitations concerning the long-term efficacy and safety due to local side effects and risk of anaphylaxis. Thus, effective and safe vaccines with reduced dose of allergen have been developed using adjuvants. Nevertheless, the use of adjuvants still has several disadvantages, which limits its use in human vaccines. In this context, several novel adjuvants for allergen immunotherapy are currently being investigated and developed. Currently, nanoparticles-based allergen-delivery systems have received much interest as potential adjuvants for allergen immunotherapy. It has been demonstrated that the incorporation of allergens into a delivery system plays an important role in the efficacy of allergy vaccines. Several nanoparticles-based delivery systems have been described, including biodegradable and nondegradable polymeric carriers. Therefore, this paper provides an overview of the current adjuvants used for allergen immunotherapy. Furthermore, nanoparticles-based allergen-delivery systems are focused as a novel and promising strategy for allergy vaccines.

show abstract

Methods for nano-particle based vaccine formulation and evaluation of their immunogenicity

Cited by 89 publications

References 20 publications

Proteoliposomes in nanobiotechnology

Proteoliposomes in nanobiotechnology

Anti-HIV-1 nanotherapeutics: promises and challenges for the future

Genetic toxicology and preliminaryin vivostudies of nitric oxide donor tocopherol analogs as potential new class of antiatherogenic agents

Contact Info

Product

Resources

About