PLoS ONE volume 9, issue 5, Pe98341 2014 DOI: 10.1371/journal.pone.0098341 View full text
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Oleh I. Petriv, Kevin A. Heyries, Michael VanInsberghe, David Walker, Carl L. Hansen

Abstract: The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Three alternative assay approaches are presented for implementing MTS analysis of chromosome 21, providing a 10-fold conce…

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