Methods for Measuring Serum Activity Levels of the 192 Q and R Isoenzymes of Paraoxonase 1 in QR Heterozygous Individuals

Teiber, John F.; Kramer, Gerald L.; Haley, Robert W.

doi:10.1373/clinchem.2012.199331

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…Three known activities of this enzyme are paraoxonase (PON), arylesterase (ARE) and diazoxonase. It provides the antiatherogenic properties of HDL, by preventing low-density lipoprotein (LDL) oxidation [ 4 , 5 ]. Paraoxonase 1 deficiency is very sensitive to development of atherosclerosis and cardiovascular disease [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Serum paraoxonase 1 activity in patients with iron deficiency anemia

Okuturlar¹,

Gedikbaşı²,

Akalın³

et al. 2016

aoms

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IntroductionIn this study we aimed to detect paraoxonase 1 (PON-1) activity in iron deficiency anemia (IDA) and to compare it with healthy controls by observing the change after iron therapy.Material and methodsIn this study, 50 adult patients with IDA and 40 healthy subjects were enrolled. All patients were analyzed at the beginning and after treatment according to laboratory assessments.ResultsMean paraoxonase and arylesterase activities in the iron deficiency anemia group were significantly lower than mean activities of the control group (102.4 ±19.2 U/l and 163.3 ±13.68 U/l, respectively and 157.3 ±26.4 U/l and 256.1 ±24.6 U/l, respectively; p = 0.0001 for both). Paraoxonase and arylesterase activities significantly increased after treatment for IDA (143.2 ±13.9 and 197.6 ±27.9 U/l, respectively, p = 0.0001). Mean activities after treatment with iron were significantly lower than mean activities in the control group (p = 0.002; p = 0.0001 respectively).ConclusionsParaoxonase and arylesterase activities in patients with IDA significantly increased after treatment with iron therapy. In adults IDA may also be one of the factors associated with increased risk of atherosclerosis.

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Section: Introductionmentioning

confidence: 99%

Serum paraoxonase 1 activity in patients with iron deficiency anemia

Okuturlar¹,

Gedikbaşı²,

Akalın³

et al. 2016

aoms

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show abstract

“…All biomarkers reported here have been previously measured and the analytical methods described, 24 including MPO, plasma lipids, serum PON1 activity, 25,26 cholesterol efflux, 27 Interleukin 18, 28 high-sensitivity C-reactive protein (hs-CRP), 24,29 asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). 30 MPO measurements were provided by Biosite, Inc. (now Alere, Inc., Waltham, MA).…”

Section: Methodsmentioning

confidence: 99%

“…32 PON1 arylesterase activity was measured by cleavage of phenyl acetate resulting in phenol formation, measured in kU/L, based on the extinction coefficient of phenol. 26 Cholesterol efflux capacity was assessed by measuring the efflux of fluorescence-labeled cholesterol from J774 macrophages to apolipoprotein B–depleted plasma in study participants, expressed as a percentage of efflux in the sample, normalized to a reference sample. 27 All the analyzed data came from samples collected at baseline.…”

Section: Methodsmentioning

confidence: 99%

Association of the serum myeloperoxidase/high-density lipoprotein particle ratio and incident cardiovascular events in a multi-ethnic population: Observations from the Dallas Heart Study

et al. 2017

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Background and aims Myeloperoxidase (MPO), a product of systemic inflammation, promotes oxidation of lipoproteins; whereas, high-density lipoprotein (HDL) exerts anti-oxidative effects in part via paraoxonase-1 (PON1). MPO induces dysfunctional HDL particles; however, the interaction of circulating levels of these measures in cardiovascular disease (CVD) has not been studied in humans. We tested whether serum levels of MPO indexed to HDL particle concentration (MPO/HDLp) are associated with increased CVD risk in a large multiethnic population sample, free of CVD at baseline. Methods Levels of MPO, HDL-C, and HDL particle concentration (HDLp) by NMR were measured at baseline in 2924 adults free of CVD. The associations of MPO/HDLp with incident ASCVD (first non-fatal myocardial infarction, non-fatal stroke, coronary revascularization, or CVD death) and total CVD were assessed in Cox proportional-hazards models adjusted for traditional risk factors. The median follow-up period was 9.4 years. Results Adjusted for sex and race/ethnicity, MPO/HDLp was associated directly with body mass index, smoking status, high-sensitivity C-reactive protein, and interleukin 18, and inversely with age, HDL-C levels, HDL size, and PON1 arylesterase activity, but not with cholesterol efflux. In fully adjusted models, the highest versus lowest quartile of MPO/HDLp was associated with a 74% increase in incident ASCVD (aHR, 1.74, 95% CI 1.12–2.70) and a 91% increase in total incident CVD (aHR, 1.91, 95% CI 1.27–2.85). Conclusions Increased MPO indexed to HDL particle concentration (MPO/HDLp) at baseline is associated with increased risk of incident CVD events in a population initially free of CVD over the 9.4 year period.

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“…It ensures the anti-atherogenic features of HDL, by avoiding low-density lipoprotein (LDL) oxidation. (15) Iron deficiency is an important health problem of women over the age of 40, which is frequently encountered in our country as well as all over the world and creates a certain burden on the health economy of a country.…”

Section: Introductionmentioning

confidence: 99%

Increased oxidative stress in adult women with iron deficiency anemia

Karabulut¹,

AVCI²,

Avcı³

2022

Universa Medicina

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BackgroundIron deficiency anemia (IDA), a type of anemia with an increasing global frequency, is more common in women than men in the population. In IDA, the sensitivity of erythrocytes to oxidants is increased and their lifespan is shortened. Oxidative stress is an imbalance between free radicals and antioxidant molecules which is one of the potential biochemical mechanisms involved in the pathogenesis of IDA. In our study, we aimed to determine the levels of oxidant and antioxidant markers by assessing the levels of total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), paraoxonase-1 (PON-1), and myeloperoxidase (MPO) in women with IDA. MethodsThis was a cross-sectional study involving 47 women with IDA aged ³40 years and 47 women volunteers. The levels of TAS, TOS, OSI, PON-1, and MPO were determined spectrophotometrically using appropriate kits. Non-parametric Mann Whitney-U tests were used to analyze the data. ResultsThe levels of antioxidants TAS (1.42 mmol Trolox equiv./L) and MPO (54.00 U/L) in the IDA group were significantly lower than in the control group [TAS (1.67 mmol Trolox equiv./L) and MPO (89.00 U/L)] (p=0.000 and p= 0.019, respectively). However, TOS (6.25 ìmol H2O2 equiv./L) level in the IDA group was significantly higher than in the control group (4.13 ìmol H2O2 equiv./L) (p=0.000), but PON-1 was not significantly different between the two groups (p=0.375). ConclusionIn women with IDA, the oxidant-antioxidant balance is impaired, resulting in oxidative stress. Therefore, IDA in adult women must receive adequate attention in clinical practice.

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Methods for Measuring Serum Activity Levels of the 192 Q and R Isoenzymes of Paraoxonase 1 in QR Heterozygous Individuals

Cited by 7 publications

References 25 publications

Serum paraoxonase 1 activity in patients with iron deficiency anemia

Serum paraoxonase 1 activity in patients with iron deficiency anemia

Association of the serum myeloperoxidase/high-density lipoprotein particle ratio and incident cardiovascular events in a multi-ethnic population: Observations from the Dallas Heart Study

Increased oxidative stress in adult women with iron deficiency anemia

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