Methods for Measuring Plasma Hemoglobin in Micromolar Concentration Compared

Fairbanks, Virgil F.; Ziesmer, Steven C.; O’Brien, Peter C.

doi:10.1093/clinchem/38.1.132

Cited by 127 publications

(76 citation statements)

References 10 publications

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“…Harboe's method together with updated validations for measuring haemoglobin (Hb) in micromolar concentrations were used (Harboe 1959;Fairbanks et al 1992). We measured absorbance with an Ultrospec Ò 3300 pro spectrophotometer (Amersham Biosciences, Cambridge, UK; acquired by General Electric Company, Fairfield, CT, USA) at wavelengths of 380 nm, 415 nm and 450 nm.…”

Section: Vitreous Haemoglobinmentioning

confidence: 99%

Increased vitreous levels of hydroimidazolone in type 2 diabetes patients are associated with retinopathy: a case‐control study

Fosmark

Bragadóttir

Stene-Johansen

et al. 2007

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: As advanced glycation endproducts (AGEs) and the vitreous body are both considered to be involved in the development of diabetic retinopathy and hydroimidazolone is one of the most prominent AGEs, we compared vitreous and serum hydroimidazolone in diabetes patients with proliferative diabetic retinopathy (PDR) to levels in non-diabetes controls, co-measuring vitreous albumin and vascular endothelial growth factor (VEGF). Methods:In a cross-sectional case-control study design, we used immunoassays to compare vitreous and serum hydroimidazolone and VEGF levels in 23 consecutive type 2 diabetes mellitus patients with a median known diabetes duration of 12 years (range 1-38 years) with those in 32 non-diabetes age-matched controls undergoing vitrectomy.Results: Vitreous hydroimidazolone was increased in the PDR group (median 1.3 U ⁄ ml, range 0.5-3.3 U ⁄ ml) compared with controls (median 0.8 U ⁄ ml, 0.5-2.5 U ⁄ ml) (p ¼ 0.026). Hydroimidazolone levels in serum and vitreous correlated (r ¼ 0.49, p ¼ 0.019). In PDR, vitreous VEGF levels were increased (median 1600 pg ⁄ ml, range 20-14 700 pg ⁄ ml) compared with controls (median 7 pg ⁄ ml, range 2-500 pg ⁄ ml) (p < 0.001). Similarly, vitreous albumin concentration was increased in PDR (median 1.6 g ⁄ l, range 0.7-3.0 g ⁄ l) compared with controls (median 0.3 g ⁄ l, range 0.08-1.9 g ⁄ l) (p < 0.001). Albumin could not explain the differences in vitreous VEGF levels in a logistic regression analysis. No correlation was found between vitreous levels of VEGF and hydroimidazolone (r ¼ 0.12, p ¼ 0.59).Conclusions: Increased vitreous hydroimidazolone is associated with diabetic retinopathy, possibly due to a blood-retinal barrier breakdown. Irrespective of origin, it may add to the ocular damage and needs further causal investigation. Increased VEGF in diabetic vitreous is probably of intraocular origin. It is not associated with vitreous hydroimidazolone.

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Section: Vitreous Haemoglobinmentioning

confidence: 99%

Increased vitreous levels of hydroimidazolone in type 2 diabetes patients are associated with retinopathy: a case‐control study

Fosmark

Bragadóttir

Stene-Johansen

et al. 2007

Acta Ophthalmologica Scandinavica

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“…Briefly, serum was diluted 11-fold in PBS and hemoglobin was measured based on the following equation: C HB = 1.65(A 415 ) -0.93(A 380 ) -0.73(A 450 ). Samples exhibited 0.03 ± 0.02 mg/mL hemoglobin (n=25), which is within the normal range (0.02 ± 0.02 mg/mL) 18 . Levels of NSE (Alpco 43-NSEHU-E01) and S100β (Millipore EZHS100B-33K) were determined by enzyme-linked immunosorbent assay following manufacturer's directions.…”

Section: Serum Analysismentioning

confidence: 59%

Systemic evidence of acute seizure-associated neuronal injury in patients with temporal lobe epilepsy

Kunda

LaFrance‐Corey

Khadjevand

et al. 2017

Preprint

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Patients with drug refractory temporal lobe epilepsy frequently accumulate cognitive impairment over time, suggesting neuronal loss induced by seizures. We measured serum levels of neuronspecific enolase (NSE), a neuronal injury marker, relative to levels of S100β, a marker of glial injury, at 6 AM, 9 AM, noon, 3 PM, and 6 PM over the course of several days in 7 epilepsy patients and 4 healthy controls. All epilepsy patients exhibited significant deviations in NSE levels through time, and 4 of the epilepsy patients exhibited large sample entropy values and large signal variation metrics for NSE relative to S100β. Controls did not exhibit such changes. Correlation analysis revealed that NSE levels were significantly elevated after clinical seizure events. There was also a highly significant relationship between increased EEG spike frequency and an increase in serum NSE levels measured 24 hours later. The detection of large but transient post-ictal increases in NSE suggests that even self-limited seizures may cause an injury to neurons that underlies cognitive decline in some patients. Post-ictal assessment of serum NSE may serve as a biomarker for measuring the efficacy of future acute neuroprotective strategies in epilepsy patients.

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“…Heparinized whole blood was incubated with SaS (10%) at 37°C in 5% CO 2 for 6 h and centrifuged, and the plasma was collected. The free haemoglobin as a classical factor for haemolysis was measured as described previously [17].…”

Section: Lysis Of Pmns and Erythrocytes By St398 Sasmentioning

confidence: 99%

Phenotyping of Staphylococcus aureus reveals a new virulent ST398 lineage

Schmidt

Zündorf

Grüger

et al. 2013

Clinical Microbiology and Infection

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Staphylococcus aureus sequence type (ST)398, which is commonly found as a colonization strain in pig farming, is emerging more frequently as the cause of human infections. In this study, we analysed ST398 of porcine and human origin at the genetic, protein and immunogenic levels. Although genetic analysis of the genes encoding the major virulence factors revealed the presence of the same genes in all strains studied, the results demonstrated spa type crossing alterations in adhesion abilities in addition to a strongly enhanced lysis activity directly linked to impaired clearance attributable to polymorphonuclear leukocytes (PMNs). This change in virulence pattern indicates high heterogenicity in the ST398 pool that is not based on a different genetic make-up, but probably on variations in the genetic regulation systems. These modifications, which are tightly connected to pathogenicity, cannot be detected by conventional diagnostic methods.

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Methods for Measuring Plasma Hemoglobin in Micromolar Concentration Compared

Cited by 127 publications

References 10 publications

Increased vitreous levels of hydroimidazolone in type 2 diabetes patients are associated with retinopathy: a case‐control study

Increased vitreous levels of hydroimidazolone in type 2 diabetes patients are associated with retinopathy: a case‐control study

Systemic evidence of acute seizure-associated neuronal injury in patients with temporal lobe epilepsy

Phenotyping of Staphylococcus aureus reveals a new virulent ST398 lineage

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